A5024249437- Autophagy in Disease and Therapy
- Mosquito-borne diseases and control
- Viral Infections and Vectors
- HIV Research and Treatment
- RNA Interference and Gene Delivery
- Toxoplasma gondii Research Studies
- Cancer Research and Treatments
- RNA regulation and disease
- HIV/AIDS Research and Interventions
- Viral Infections and Outbreaks Research
- interferon and immune responses
- Asphalt Pavement Performance Evaluation
- Cellular transport and secretion
- CRISPR and Genetic Engineering
- Hemoglobinopathies and Related Disorders
- Infrastructure Maintenance and Monitoring
- Vibrio bacteria research studies
- Animal Disease Management and Epidemiology
- Virus-based gene therapy research
- Geotechnical Engineering and Underground Structures
- Genetics and Neurodevelopmental Disorders
- Virology and Viral Diseases
- Energy and Environment Impacts
- Vector-Borne Animal Diseases
- Metabolism and Genetic Disorders
Inserm
2013-2023
Centre National de la Recherche Scientifique
2013-2023
Institut Cochin
2023
Université Paris Cité
2023
University Medical Center Groningen
2017-2022
University of Groningen
2017-2022
École Normale Supérieure de Lyon
2011-2022
Centre International de Recherche en Infectiologie
2013-2022
Université Claude Bernard Lyon 1
2013-2022
Weatherford College
2015
The interplay between autophagy and intracellular pathogens is intricate as an essential cellular response to fight against infections, whereas numerous microbes have developed strategies escape this process or even exploit it their own benefit. fine tuned timing and/or selective molecular pathways involved in the induction of upon infections could be cornerstone allowing cells either control pathogens, invaded by them. We report here that measles virus infection induces successive...
During macroautophagy/autophagy, precursor cisterna known as phagophores expand and sequester portions of the cytoplasm and/or organelles, subsequently close resulting in double-membrane transport vesicles called autophagosomes. Autophagosomes fuse with lysosomes/vacuoles to allow degradation recycling their cargoes. We previously showed that sequential binding yeast Atg2 Atg18 Atg9, only conserved transmembrane protein autophagy, at extremities phagophore mediates establishment membrane...
Autophagy is a potent cell autonomous defense mechanism that engages the lysosomal pathway to fight intracellular pathogens. Several autophagy receptors can recognize invading pathogens in order target them towards for their degradation after fusion of pathogen-containing autophagosomes with lysosomes. However, numerous avoid or exploit autophagy, among which measles virus (MeV). This induces complete flux, required improve viral replication. We therefore asked how interferes during course...
Viruses have evolved unique strategies to evade or subvert autophagy machinery. Enterovirus A71 (EV-A71) induces during infection in vitro and vivo. In this study, we report that EV-A71 triggers autolysosome formation human rhabdomyosarcoma (RD) cells facilitate its replication. Blocking autophagosome-lysosome fusion with chloroquine inhibited virus RNA replication, resulting lower viral titres, copies proteins. Overexpression of the non-structural protein 2BC induced formation. Yeast...
Limitation of excessive inflammation due to selective degradation pro-inflammatory proteins is one the cytoprotective functions attributed autophagy. In current study, we highlight that autophagy also plays a vital role in promoting establishment robust inflammatory response. Under conditions, here TLR3-activation by poly(I:C) treatment, repressor TNIP1 (TNFAIP3 interacting protein 1) phosphorylated Tank-binding kinase 1 (TBK1) activating an LIR motif leads autophagy-dependent TNIP1,...
Bone marrow stromal antigen 2 (BST2)/tetherin is a restriction factor that reduces HIV-1 dissemination by tethering virus at the cell surface. BST2 also acts as sensor of budding, establishing cellular antiviral state. The Vpu protein antagonizes functions via multiple mechanisms, including subversion an LC3C-associated pathway, key intrinsic antimicrobial mechanism. Here, we describe first step this viral-induced process. This process initiated plasma membrane through recognition and...
Selective types of autophagy mediate the clearance specific cellular components and are essential to maintain homeostasis. However, tools directly induce monitor such pathways limited. Here we introduce PIM (particles induced by multimerization) assay as a tool for study aggrephagy, autophagic aggregates. The uses an inducible multimerization module assemble protein clusters, which upon induction recruit ubiquitin, p62, LC3 before being delivered lysosomes. Moreover, use dual fluorescent tag...
Abstract The NF-κB pathway is constitutively activated in adult T cell leukemia, an aggressive malignancy caused by Human Leukemia Virus type 1 (HTLV-1). viral oncoprotein Tax triggers this constitutive activation interacting with the ubiquitin-rich IKK complex. We previously demonstrated that Optineurin and TAX1BP1, two members of ubiquitin-binding, Sequestosome-1 (SQSTM-1/p62)-like selective autophagy receptor family, are involved Tax-mediated signaling. Here, using a proximity-dependent...
Although it is admitted that secondary infection can complicate viral diseases, the consequences of on cell susceptibility to other infections remain underexplored at cellular level. We though examine whether sustained macroautophagy/autophagy associated with measles virus (MeV) could help cells oppose invasion by Salmonella Typhimurium, a bacterium sensitive autophagic restriction. report here unexpected finding markedly replicated in MeV-infected cultures due selective growth within...
Crimean-Congo hemorrhagic fever virus (CCHFV) is a that causes severe liver dysfunctions and fever, with high mortality rate. Here, we show CCHFV infection caused massive lipidation of LC3 in hepatocytes. This was not dependent on ATG5, ATG7 or BECN1, no signs for recruitment the alternative ATG12-ATG3 pathway found. Both replication protein synthesis were required LC3. Despite an augmented transcription SQSTM1, amount proteins did sustained increase infected cells, indicating degradation...
Dugbe orthonairovirus (DUGV) is a tick-borne arbovirus within the order Bunyavirales. Although displaying mild pathogenic potential, DUGV genetically related to Crimean–Congo hemorrhagic fever virus (CCHFV), another that causes severe liver dysfunction and with high mortality rate in humans. As we previously observed CCHFV infection could massively recruit lipidate MAP1LC3 (LC3), core factor involved autophagic degradation of cytosolic components, asked whether also substantially impacts...
ATG13 and FIP200 are two subunits of the ULK kinase complex, a key regulatory component autophagy machinery. We have previously found that FIP200-ATG13 subcomplex controls picornavirus replication outside its role in complex autophagy. Here, we characterized HSBP1, very small cytoplasmic coiled-coil protein, as novel interactor binds these proteins via FIP200. HSBP1 is pro-picornaviral host factor since knockdown or knockout, inhibits various picornaviruses. The anti-picornaviral function...
Abstract BST2/Tetherin is a restriction factor that reduces HIV-1 dissemination by tethering virus at the cell surface. BST2 also acts as sensor of budding, establishing cellular anti-viral state. The Vpu protein antagonizes antiviral functions, notably subverting an LC3C-associated pathway, key intrinsic anti-microbial mechanism. Here, we show ATG5 associates with and signaling scaffold to trigger pathway induced infection. This process initiated plasma membrane through recognition...
On estime aujourd’hui que pres d’un cancer sur cinq a une origine infectieuse. Avec la decouverte de nouveaux agents infectieux, ce chiffre est probablement appele augmenter dans les annees venir. En 2006, nouveau γ-retrovirus des biopsies tumeur prostatique amorca intense recherche : virus, apparente aux retrovirus murins xenotropes et baptise xenotropic MLV-related virus (XMRV), serait-il cause du prostate ? Cinq ans plus tard, l’engouement retrovirologistes retombe. Un un, arguments en...
Viruses represent an important cause of cancer in humans: infections are estimated to account for close one case out five.With the ongoing discovery new infectious agents, this number should be raising near future. In 2006, a _-retrovirus prostate biopsies launched intense research activity: could xenotropic MLV-related virus (XMRV) cancer? Five years later, initial enthusiasm retrovirologists has dramatically diminished. One by one, arguments favouring hypothesis human infection with XMRV...