A5050962142- Microtubule and mitosis dynamics
- Cellular Mechanics and Interactions
- Cellular transport and secretion
- Ubiquitin and proteasome pathways
- Advanced Electron Microscopy Techniques and Applications
- Cell Image Analysis Techniques
- Advanced Neuroimaging Techniques and Applications
- Advanced NMR Techniques and Applications
- Advanced Fluorescence Microscopy Techniques
- Photosynthetic Processes and Mechanisms
- Micro and Nano Robotics
- Autophagy in Disease and Therapy
- 14-3-3 protein interactions
- Protist diversity and phylogeny
- Genetics, Aging, and Longevity in Model Organisms
- Neurogenesis and neuroplasticity mechanisms
- Mosquito-borne diseases and control
- Biopolymer Synthesis and Applications
- Pancreatic function and diabetes
- Zeolite Catalysis and Synthesis
- Earth Systems and Cosmic Evolution
- Cancer Treatment and Pharmacology
- Mesoporous Materials and Catalysis
- Hippo pathway signaling and YAP/TAZ
- Endoplasmic Reticulum Stress and Disease
Utrecht University
2016-2025
University Medical Center Utrecht
2025
University of California, Davis
2021
University of Pittsburgh
2013
Microtubules are hollow biopolymers of 25-nm diameter and key constituents the cytoskeleton. In neurons, microtubules organized differently between axons dendrites, but their precise organization in different compartments is not completely understood. Super-resolution microscopy techniques can detect specific structures at an increased resolution, narrow spacing neuronal poses challenges because most existing labelling strategies increase effective microtubule by 20-40 nm will thereby blend...
The first evidence of micro- and nanoplastic (MNP) exposure in the human placenta is emerging. However, toxicokinetics toxicity MNPs placenta, specifically environmentally relevant particles, remain unclear.We examined transport, uptake, pristine experimentally weathered nonsyncytialized syncytialized BeWo b30 choriocarcinoma cells.We performed untargeted chemical characterization using liquid chromatography high-resolution mass spectrometry to evaluate compositional differences following...
Kinesin-1 is responsible for microtubule-based transport of numerous cellular cargoes. Here, we explored the regulation kinesin-1 by MAP7 proteins. We found that all four mammalian family members bind to kinesin-1. In HeLa cells, MAP7, MAP7D1, and MAP7D3 act redundantly enable kinesin-1–dependent microtubule recruitment truncated KIF5B-560, which contains stalk but not cargo-binding autoregulatory regions. vitro, purified increase landing rate processivity through transient association with...
Microtubule-targeting agents (MTAs) are widely used for treatment of cancer and other diseases, a detailed understanding the mechanism their action is important development improved microtubule-directed therapies. Although there large body data on interactions different MTAs with purified tubulin microtubules, much less known about how effects modulated by microtubule-associated proteins. Among regulatory factors potential to have strong impact MTA activity microtubule plus end-tracking...
The dynamic instability of microtubules plays a key role in controlling their organization and function, but the cellular mechanisms regulating this process are poorly understood. Here, we show that cytoplasmic linker-associated proteins (CLASPs) suppress transitions from microtubule growth to shortening, termed catastrophes, including those induced by microtubule-destabilizing agents physical barriers. Mammalian CLASPs encompass three TOG-like domains, TOG1, TOG2, TOG3, none which bind free...
Centrioles are fundamental and evolutionarily conserved microtubule-based organelles whose assembly is characterized by microtubule growth rates that orders of magnitude slower than those cytoplasmic microtubules. Several centriolar proteins can interact with tubulin or microtubules, but how they ensure the exceptionally slow microtubules has remained mysterious. Here, we bring together crystallographic, biophysical, reconstitution assays to demonstrate human protein CPAP (SAS-4 in worms...
Intracellular transport relies on multiple kinesins, but it is poorly understood which kinesins are present particular cargos, what their contributions and whether they act simultaneously the same cargo. Here, we show that Rab6-positive secretory vesicles transported from Golgi apparatus to cell periphery by kinesin-1 KIF5B kinesin-3 KIF13B, determine location of secretion events. plays a dominant role, whereas KIF13B helps Rab6 reach freshly polymerized microtubule ends, binds poorly,...
Abstract γ-Tubulin ring complex (γ-TuRC) is the major microtubule-nucleating factor. After nucleation, microtubules can be released from γ-TuRC and stabilized by other proteins, such as CAMSAPs, but biochemical cross-talk between minus-end regulation pathways poorly understood. Here we reconstituted this process in vitro using purified components. We found that all CAMSAPs could bind to minus ends of γ-TuRC-attached microtubules. CAMSAP2 CAMSAP3, which decorate stabilize growing not tracking...
Microtubules control different aspects of cell polarization. In cells with a radial microtubule system, pivotal role in setting up asymmetry is attributed to the relative positioning centrosome and nucleus. Here, we show that loss had no effect on ability endothelial polarize move 2D 3D environments. contrast, non-centrosomal microtubules stabilized by minus-end-binding protein CAMSAP2 were required for directional migration substrates establishment polarized morphology soft matrices. was...
The cytoplasm is a highly complex and heterogeneous medium that structured by the cytoskeleton. How local transport depends on organization dynamics of F-actin microtubules poorly understood. Here we use novel delivery functionalization strategy to utilize quantum dots (QDs) as probes for active passive intracellular transport. Rapid imaging non-functionalized QDs reveals two populations with 100-fold difference in diffusion constant, faster fraction increasing upon actin depolymerization....
Axon regeneration after injury requires the extensive reconstruction, reorganization, and stabilization of microtubule cytoskeleton in growth cones. Here, we identify KIF3C as a key regulator axonal by controlling dynamics organization cone. is developmentally regulated. Rat embryonic sensory axons cones contain undetectable levels protein that locally translated immediately injury. In adult neurons, axonally transported from cell body enriched at cone where it preferentially binds to...
Highlights•Ankyrin-G in complex with EBs recruits microtubule bundles to the plasma membrane•TRIM46 is a rescue factor that forms stable parallel bundles•TRIM46-bound microtubules direct Neurofascin-186 trafficking proximal axon•Ankyrin-G controls retention axon initial segmentSummaryThe segment (AIS) unique neuronal compartment plays crucial role generation of action potential and polarity. The assembly AIS requires membrane, scaffolding, cytoskeletal proteins, including Ankyrin-G TRIM46....
Article23 August 2019Open Access Source DataTransparent process VAP-SCRN1 interaction regulates dynamic endoplasmic reticulum remodeling and presynaptic function Feline W Lindhout orcid.org/0000-0001-5075-5434 Department of Biology, Cell Utrecht University, Utrecht, The Netherlands Search for more papers by this author Yujie Cao Josta T Kevenaar Anna Bodzęta Riccardo Stucchi Biomolecular Mass Spectrometry Proteomics, Bijvoet Center Research Institute Pharmaceutical Sciences, Maria M...
Microtubules are dynamic polymers that in cells can grow, shrink or pause, but the factors promote pausing poorly understood. Here, we show mammalian kinesin-4 KIF21B is a processive motor accumulate at microtubule plus ends and induce pausing. A few molecules sufficient to strong growth inhibition of end vitro. This property depends on non-motor microtubule-binding domains located stalk region C-terminal WD40 domain. The WD40-containing tail displays preference for GTP-type over GDP-type...