A5058577736- Microtubule and mitosis dynamics
- Neurobiology and Insect Physiology Research
- Cellular Mechanics and Interactions
- Ubiquitin and proteasome pathways
- Biopolymer Synthesis and Applications
- Cellular transport and secretion
- Food composition and properties
- Probiotics and Fermented Foods
- Food Science and Nutritional Studies
- Retinal Development and Disorders
- Phytochemicals and Antioxidant Activities
- Food Quality and Safety Studies
- RNA regulation and disease
- Neuroscience and Neural Engineering
- Microencapsulation and Drying Processes
- Spaceflight effects on biology
- Humic Substances and Bio-Organic Studies
- Polysaccharides Composition and Applications
- Bacterial Genetics and Biotechnology
- Protein Hydrolysis and Bioactive Peptides
- Complementary and Alternative Medicine Studies
- Microbial Metabolites in Food Biotechnology
- Agriculture, Water, and Health
- Nuclear Structure and Function
- Livestock Management and Performance Improvement
Utrecht University
2019-2024
Chhatrapati Shahu Ji Maharaj University
2018-2024
Banaras Hindu University
2017-2018
Tata Institute of Fundamental Research
2018
Indian Institute of Technology Bombay
2008
The assembly and stability of FtsZ protofilaments have been shown to play critical roles in bacterial cytokinesis. Recent evidence suggests that may be considered as an important antibacterial drug target. Curcumin, a dietary polyphenolic compound, has potent activity against number pathogenic bacteria including Staphylococcus aureus, epidermidis Enterococcus. We found curcumin induced filamentation the Bacillus subtilis 168, suggesting it inhibits Further, strongly inhibited formation...
Abstract γ-Tubulin ring complex (γ-TuRC) is the major microtubule-nucleating factor. After nucleation, microtubules can be released from γ-TuRC and stabilized by other proteins, such as CAMSAPs, but biochemical cross-talk between minus-end regulation pathways poorly understood. Here we reconstituted this process in vitro using purified components. We found that all CAMSAPs could bind to minus ends of γ-TuRC-attached microtubules. CAMSAP2 CAMSAP3, which decorate stabilize growing not tracking...
Highlights•Ankyrin-G in complex with EBs recruits microtubule bundles to the plasma membrane•TRIM46 is a rescue factor that forms stable parallel bundles•TRIM46-bound microtubules direct Neurofascin-186 trafficking proximal axon•Ankyrin-G controls retention axon initial segmentSummaryThe segment (AIS) unique neuronal compartment plays crucial role generation of action potential and polarity. The assembly AIS requires membrane, scaffolding, cytoskeletal proteins, including Ankyrin-G TRIM46....
Microtubules play a key role in cell division, motility, and intracellular trafficking. Microtubule lattices are generally regarded as stable structures that undergo turnover through dynamic instability of their ends [1Desai A. Mitchison T.J. polymerization dynamics.Annu. Rev. Cell Dev. Biol. 1997; 13: 83-117Crossref PubMed Scopus (1955) Google Scholar]. However, recent evidence suggests microtubules also exchange tubulin dimers at the sites lattice defects, which can be induced by...
Rab4 GTPase, essential for endosomal sorting and trafficking, is implicated in synaptic atrophy dementia. To uncover the underlying mechanism, we studied correlation between vesicle transport axons episodic remodeling of synapses central nervous system (CNS) Drosophila larvae. We found that synapse-bound traffic presynaptic enrichment vesicles increase during programmed, transient contraction ventral neuropil region at a specific larval stage. This coincides with activation insulin...
Micronutrient deficiencies, particularly in essential vitamins and minerals, pose a significant public health challenge, affecting over two billion people worldwide. These deficiencies contribute to various issues, impaired cognitive development, reduced productivity, ultimately hindering social economic progress. Biofortification, process of enhancing the nutritional content staple crops through conventional breeding or genetic engineering, has emerged as promising sustainable approach...
Abstract Cell mechano-sensation and adaptation are supported by the actin network. The microtubule network is not considered to be directly sensitive mechanical forces acting on a cell. However, recent studies isolated microtubules in vitro have shown that bending an impact their structure, composition lifespan, suggesting that, cellular context, may react forces. We tested this hypothesis living cells subjecting them cycles of compressive found became distorted, less dynamic more stable....
Synapse density is an essential indicator of development and functioning the central nervous system. It estimated indirectly through accumulation pre postsynaptic proteins in tissue sections. 3D reconstruction electron microscopic images serial sections one most definitive means estimating formation active synapses brain. tedious highly skill-dependent. Confocal imaging whole mounts or thick brain provides a natural alternative for rapid gross estimation synapse large areas. The optical...
Abstract γ-tubulin ring complex (γ-TuRC) is the major microtubule-nucleating factor. After nucleation, microtubules can be released from γ-TuRC and stabilized by other proteins, such as CAMSAPs, but biochemical cross-talk between minus-end regulation pathways poorly understood. Here, we reconstituted this process in vitro using purified components. We found that all CAMSAP proteins could bind to minus-ends of γ-TuRC-attached microtubules. CAMSAP2 CAMSAP3, which decorate stabilize growing...
Summary Microtubule network plays a key role in cell division, motility and intracellular trafficking. lattices are generally regarded as stable structures that undergo turnover through dynamic instability of their ends [1]. However, recent evidence suggests microtubules also exchange tubulin dimers at the sites lattice defects, which can either be induced by mechanical stress or occur spontaneously during polymerization [2–4]. Tubulin incorporation restore microtubule integrity; moreover,...
The present investigation was undertaken to explore the possibility of use soy milk in litchi pulp based shrikhand produce a novel fermented product. product prepared as per standard method using constant level (15%) and sugar (40%). Different levels (10, 20, 30 40%) were tried substitute standardized milk. It found that incorporation at 20% slightly improved colour appearance scores developed Other sensory parameter (Flavour, Body texture, Sweetness Overall Acceptability) decreased...
Response surface methodology was employed to find out optimum proportions of flaxseed powder, mango pulp, synbiotic microcapsules develop fortified <i>Dahi</i>. The quadratic model fitted antioxidant activity, firmness (g), cohesiveness (gs), whey separation (%), and probiotic viable count (CFU/g) runs as the responses. Analysis variance revealed that models were well adjusted predict experimental data. Determination coefficients (R<sup>2</sup>) higher than 90% which showed developed fitted...