A5084943136- Cancer, Hypoxia, and Metabolism
- Cancer Cells and Metastasis
- Advanced Fluorescence Microscopy Techniques
- Cellular Mechanics and Interactions
- Nuclear Structure and Function
- Microtubule and mitosis dynamics
- Cancer Research and Treatments
- RNA Research and Splicing
- Protist diversity and phylogeny
- Cell Adhesion Molecules Research
- Cell Image Analysis Techniques
- Optical Imaging and Spectroscopy Techniques
- Hemoglobin structure and function
- Immune cells in cancer
- Metabolomics and Mass Spectrometry Studies
- RNA and protein synthesis mechanisms
- Cell death mechanisms and regulation
- Immunotherapy and Immune Responses
- Pancreatic and Hepatic Oncology Research
- Connexins and lens biology
- RNA modifications and cancer
- Chemokine receptors and signaling
- Genetic factors in colorectal cancer
- Viral Infectious Diseases and Gene Expression in Insects
- Advanced Proteomics Techniques and Applications
Institut Curie
2019-2025
Institut Pierre-Gilles de Gennes pour la Microfluidique
2019-2025
Centre National de la Recherche Scientifique
2015-2025
Université Paris Sciences et Lettres
2019-2025
Institut Necker Enfants Malades
2025
École Normale Supérieure - PSL
2022
Chimie ParisTech
2022
Laboratoire de Biologie Moléculaire et Cellulaire des Eucaryotes
2022
Centre de Recherche en Cancérologie de Lyon
2014-2020
Centre Léon Bérard
2016-2020
The RNA helicases DDX5 and DDX17 are members of a large family highly conserved proteins that involved in gene-expression regulation; however, their vivo targets activities biological processes such as cell differentiation, which requires reprogramming programs at multiple levels, not well characterized.Here, we uncovered mechanism by cooperate with heterogeneous nuclear ribonucleoprotein (hnRNP) H/F splicing factors to define epithelial-and myoblast-specific subprograms.We then observed...
Abstract Intracellular Ca 2+ signaling regulates cell migration by acting on cytoskeleton architecture, directionality and focal adhesions dynamics. In migrating cells, cytosolic pool pulses are described as key components of these effects. Whereas the role mitochondrial calcium homeostasis Mitochondria Cacium Uniporter (MCU) in were recently highlighted vivo using zebrafish model, their implication actin cystokeleton dynamics mammals is not totally characterized. Here, we show that mcu...
Abstract Here we present a method to reduce the photobleaching of fluorescent proteins and associated phototoxicity. It exploits photophysical process known as reverse intersystem crossing, which induce by near-infrared co-illumination during fluorophore excitation. This dual illumination reduces effects 1.5–9.2-fold, can be easily implemented on commercial microscopes is effective in eukaryotic prokaryotic cells with wide range proteins.
To reach inflamed tissues from the circulation, neutrophils must overcome physical constraints imposed by tissue architecture, such as endothelial barrier or three-dimensional (3D) interstitial space. In these microenvironments, are forced to migrate through spaces smaller than their own diameter. One of main challenges for cell passage narrow gaps is deformation nucleus, largest and stiffest organelle in cells. Here, we showed that chemokines, extracellular signals guide migration vivo,...
Errors during cell division lead to aneuploidy, which is associated with genomic instability and transformation. In response cells activate the tumour suppressor p53 elicit a surveillance mechanism that halts proliferation promotes senescence. The molecular sensors trigger this checkpoint are unclear. Here, using tunable system of chromosome mis-segregation, we show mitotic errors nuclear deformation, softening, lamin heterochromatin alterations, leading rapid p53/p21 activation upon exit in...
ABSTRACT As the first responders of immune system, neutrophils rapidly reach inflamed tissues within minutes, navigating intricate network blood capillaries. Therefore, their ability to effectively control infections hinges on capacity squeeze and migrate through micrometric tubes that drain affected area. To understand this process, we investigated neutrophil migration in capillary tubes. In vivo , observed crawling at site infection was largely independent size capillaries they traversed....
The Transforming growth factor β (TGFβ) signaling has a paradoxical role in cancer development and outcome. Besides, the prognostic significance of TGFβ1, SMAD4 breast patients is an area many contradictions. transcriptional intermediary 1γ (TIF1γ) thought to interact with TGFβ/SMAD through different mechanisms. Our study aims define TIF1γ expression detect possible interactions among those markers that might affect outcome.Immunohistochemistry was performed on tissue microarray (TMA) blocks...
Aerotaxis or chemotaxis to oxygen was described in bacteria 130 years ago. In eukaryotes, the main adaptation hypoxia currently relies on HIF transcription factors. To investigate whether aerotaxis is conserved higher an approach based self-generation of after cell confinement developed. We show that epithelial cells from various tissues migrate with extreme directionality towards escape hypoxia, independently pathway. provide evidence that, concomitant gradient, a gradient reactive species...
Abstract Cell mechano-sensation and adaptation are supported by the actin network. The microtubule network is not considered to be directly sensitive mechanical forces acting on a cell. However, recent studies isolated microtubules in vitro have shown that bending an impact their structure, composition lifespan, suggesting that, cellular context, may react forces. We tested this hypothesis living cells subjecting them cycles of compressive found became distorted, less dynamic more stable....
We will present a new method to reduce the photobleaching of fluorescent proteins and associated phototoxicity. Our exploits photophysical process known as reverse intersystem crossing, which we induce by near-infrared co-illumination during fluorophore excitation. This dual illumination typically reduces effects 4-fold, can be easily implemented on commercial microscopes is effective in eukaryotic prokaryotic cells with wide range proteins.
Abstract Background Aerotaxis, the chemotactism to oxygen, is well documented in prokaryotes. We previously reported for first time that non-tumorigenic breast epithelial cells also display unequivocal directional migration towards oxygen. This process independent of hypoxia-inducible factor (HIF)/prolyl hydroxylase domain (PHD) pathway but controlled by redox regulation epidermal growth receptor (EGFR), with a reactive oxygen species (ROS) gradient overlapping at low concentration. Since...
Abstract Orchestrated recruitment of neutrophils to inflamed tissue is essential during initiation inflammation. Inflamed areas are usually hypoxic, and adaptation reduced oxygen pressure typically mediated by hypoxia pathway proteins. However, it still unclear how these factors influence the migration at site inflammation either their transmigration through blood-endothelial cell barrier, or motility in interstitial space. Here, we reveal that activation Hypoxia Inducible Factor-2 (HIF2α)...
Abstract Background: Aerotaxis, the chemotactism to oxygen, is well documented in prokaryotes. We previously reported for first time that non-tumorigenic breast epithelial cells also display unequivocal directional migration towards oxygen. This process independent of hypoxia-inducible factor (HIF)/prolyl hydroxylase domain (PHD) pathway but controlled by redox regulation epidermal growth receptor (EGFR), with a reactive oxygen species (ROS) gradient overlapping at low concentration. Since...