A5030307976- PI3K/AKT/mTOR signaling in cancer
- Genomics, phytochemicals, and oxidative stress
- Cell death mechanisms and regulation
- Mechanisms of cancer metastasis
- Endoplasmic Reticulum Stress and Disease
- Cancer Cells and Metastasis
- RNA Research and Splicing
- Cancer, Hypoxia, and Metabolism
- Click Chemistry and Applications
- Mitochondrial Function and Pathology
- Viral-associated cancers and disorders
- Cancer Research and Treatments
- TGF-β signaling in diseases
- HIV Research and Treatment
- RNA and protein synthesis mechanisms
- Autophagy in Disease and Therapy
- Lymphoma Diagnosis and Treatment
- RNA modifications and cancer
- Redox biology and oxidative stress
- HIV/AIDS drug development and treatment
- Genetic factors in colorectal cancer
- Slime Mold and Myxomycetes Research
- Influenza Virus Research Studies
- T-cell and Retrovirus Studies
- Growth Hormone and Insulin-like Growth Factors
Centre de Recherche en Cancérologie de Lyon
2012-2024
Centre Léon Bérard
2010-2024
Université Claude Bernard Lyon 1
2011-2024
Inserm
2011-2024
Centre National de la Recherche Scientifique
2010-2024
Centro de Investigación del Cáncer
2023
Cancer Research Center
2023
Weatherford College
2022
Université de Montpellier
2021
Institut de Recherche en Infectiologie de Montpellier
2021
Research Article5 April 2011Open Access Down-regulation of BRCA1 expression by miR-146a and miR-146b-5p in triple negative sporadic breast cancers Amandine I. Garcia CNRS UMR5286 Inserm U1052, "Equipe Labellisée LIGUE 2008", University Lyon 1, Cancer Center Lyon, Centre Léon Bérard, France Search for more papers this author Monique Buisson Pascale Bertrand CNRS-CEA UMR 217, Institut de Radiobiologie Cellulaire et Moléculaire, Fontenay-aux-Roses, Ruth Rimokh Etienne Rouleau Laboratoire...
Journal Article A general and fast method to generate multiple site directed mutations Get access Ivan Mikaelian, Mikaelian ENS-CNRS UMR49, Ecole Normale Superieure de Lyon46 allée d'Italie, 69364 Lyon Cedex 07, France Search for other works by this author on: Oxford Academic PubMed Google Scholar Alain Sergeant * To whom correspondence should be addressed Nucleic Acids Research, Volume 20, Issue 2, 25 January 1992, Page 376, https://doi.org/10.1093/nar/20.2.376 Published: 1992 history...
Dicer, a ribonuclease, is the key enzyme required for biogenesis of microRNAs and small interfering RNAs essential both mammalian development cell differentiation. Recent evidence indicates that Dicer may also be involved in tumourigenesis. However, no studies have examined clinical significance at RNA protein levels breast cancer. In this study, biological prognostic value expression was assessed cancer lines, progression cellular models, two well-characterised sets carcinoma samples...
Influenza virus infections are major public health threats due to their high rates of morbidity and mortality. Upon influenza entry, host cells experience modifications endomembranes, including those used for trafficking replication. Here we report that infection modifies mitochondrial morphodynamics by promoting mitochondria elongation altering endoplasmic reticulum-mitochondria tethering in cells. Expression the viral RNA recapitulates these inside Virus induced hyper-elongation was...
Abstract Apoptosis plays a role in cell homeostasis both normal development and disease. Bcl-xL, member of the Bcl-2 family proteins, regulates intrinsic mitochondrial pathway apoptosis. It is overexpressed several cancers. Bcl-xL has dual subcellular localisation found at mitochondria as well endoplasmic reticulum (ER). However, biological significance its ER unclear. In order to decipher functional contributions reticular pools we generated genetically modified mice expressing exclusively...
The Epstein-Barr virus early protein EB2 (also called BMLF1, Mta, or SM), a absolutely required for the production of infectious virions, shares properties with mRNA export factors. By using yeast two-hybrid screen, we have identified human OTT3 as an EB2-interacting factor. is new member Spen (split end) family proteins (huSHARP, huOTT1, DmSpen, and muMINT), which are characterized by several N-terminal RNA recognition motifs highly conserved C-terminal SPOC (Spen Paralog Ortholog...
The switch from latency to a productive infection in EBV-infected B cells is linked the expression of two viral sequence-specific DNA-binding transcription factors called EB1 and A. shares sequence homologles with bZIP family proteins In basic region required for specific DNA Interaction. Here, we provide evidence that R can synergistically activate transcription, overexpressed, unbound EB1, represses R-lnduced ('squelching'). order identify domains Involved transcriptional activation,...
Epithelial-to-mesenchymal transition (EMT) is a transdifferentiation process that converts epithelial cells into highly motile mesenchymal cells. This physiologic occurs largely during embryonic development but aberrantly reactivated in different pathologic situations, including fibrosis and cancer. We conducted siRNA screening targeted to the human kinome with aim of discovering new EMT effectors. With this approach, we have identified mTOR complex 1 (mTORC1), nutrient sensor controls...
Abstract Drug resistance and metastatic relapse remain a top challenge in breast cancer treatment. In this study, we present preclinical evidence for strategy to eradicate advanced cancers by targeting the BCL-2 homolog Nrh/BCL2L10, which discovered be overexpressed >45% of large cohort invasive carcinomas. Nrh expression these tumors correlated with reduced metastasis-free survival, determined it an independent marker poor prognosis. protein localized endoplasmic reticulum....
The progesterone receptor (PR) is an inducible transcription factor that plays critical roles in female reproductive processes and several aspects of breast cancer tumorigenesis. Our report describes the type I protein arginine methyltransferase 1 (PRMT1) as a cofactor controlling pathway, through direct methylation PR. Mechanistic assays cells indicate PRMT1 methylates PR at 637 reduces stability receptor, thereby accelerating its recycling finally transcriptional activity. Depletion...
Using a self-generated hypoxic assay, we show that the amoeba Dictyostelium discoideum displays remarkable collective aerotactic behavior. When cell colony is covered, cells quickly consume available oxygen (O2) and form dense ring moving outwards at constant speed density. To decipher this process, combined two technological developments: porphyrin-based O2 -sensing films microfluidic gradient generators. We showed exhibit aerokinetic response in low range of concentration indicative very...
ABSTRACT Human herpesviruses encode posttranscriptional activators that are believed to up-regulate viral replication by facilitating early and late gene expression. We have reported previously the Epstein-Barr virus protein EB2 (also called M or SM) promotes nuclear export of RNAs poor substrates for spliceosome assembly, an effect closely resembles human immunodeficiency type 1 Rev-dependent unspliced RNA. Here we present experimental data showing efficiently RNA expressed from a Rev...
TIF1γ, a new actor of TGFβ signaling, inhibits the Smad4-mediated response by interaction with Smad2/3 or ubiquitination Smad4. We have shown that TIF1γ participates in signaling as negative regulator Smad4 during TGFβ-induced epithelial-to-mesenchymal transition mammary epithelial cells and terminal differentiation alveolar lactation. demonstrate here is sumoylated interacts Ubc9, only known SUMO-conjugating enzyme. Four functional sumoylation sites lie within middle domain Smad domain....
The Epstein-Barr virus BZLF1 gene product EB1 (also called ZEBRA and Zta), is a transcription factor belonging to the bZIP (basic domain leucine zipper) family of nuclear proteins. Translocation nucleus (J. Becker, U. Leser, M. Marschall, A. Langford, W. Jilg, H. Gelderblom, P. Reichart, Wolf, Proc. Natl. Acad. Sci. USA 88:8332-8336, 1991) two other proteins, c-Jun c-Fos (P. Roux, J.-M. Blanchard, Fernandez, N. Lamb, Jeanteur, Piechaczyk, Cell 63:341-351, 1990), has been shown be subject...
Aerotaxis or chemotaxis to oxygen was described in bacteria 130 years ago. In eukaryotes, the main adaptation hypoxia currently relies on HIF transcription factors. To investigate whether aerotaxis is conserved higher an approach based self-generation of after cell confinement developed. We show that epithelial cells from various tissues migrate with extreme directionality towards escape hypoxia, independently pathway. provide evidence that, concomitant gradient, a gradient reactive species...
The HIV-1 Rev is a shuttling protein required for the nuclear export of unspliced and partially spliced viral mRNA. In this study, we have identified new Rev-interacting protein, that specifically interacts with signal both in yeast mammalian cells. This has features found nucleoporins including many phenylalanine-glycine repeats, very high serine content, putative zinc finger, coiled-coil domain; thus called it NLP-1 (nucleoporin-likeprotein 1). addition, gene expression analysis wheat germ...
Lysosomal regulation is a poorly understood mechanism that central to degradation and recycling processes. Here we report LAMTOR1 (late endosomal/lysosomal adaptor, MAPK mTOR activator 1) downregulation affects lysosomal activation, through mechanisms are not solely due mTORC1 inhibition. depletion strongly increases structures display scattered intracellular positioning. Despite their altered positioning, those dispersed remain overall functional: (i) the trafficking maturation of enzyme...
Transforming growth factor β (TGFβ) is widely recognised as an important that regulates many steps of normal mammary gland (MG) development, including branching morphogenesis, functional differentiation and involution. Tif1γ has previously been reported to temporally spatially control TGFβ signalling during early vertebrate development by exerting negative effects over SMAD4 availability. To evaluate the contribution Tif1 γ MG we developed a Cre/LoxP system specifically invalidate Tif1g gene...