A5062388936- Microtubule and mitosis dynamics
- Cellular Mechanics and Interactions
- Micro and Nano Robotics
- Protist diversity and phylogeny
- Photosynthetic Processes and Mechanisms
- Ubiquitin and proteasome pathways
- Advanced Materials and Mechanics
- Plant Molecular Biology Research
- Supramolecular Chemistry and Complexes
- Cellular transport and secretion
- Plant Reproductive Biology
- Amyotrophic Lateral Sclerosis Research
- Cell Image Analysis Techniques
- Magnetic and Electromagnetic Effects
- Molecular Communication and Nanonetworks
- Endoplasmic Reticulum Stress and Disease
- Polysaccharides and Plant Cell Walls
- Plant nutrient uptake and metabolism
- 3D Printing in Biomedical Research
- Cardiomyopathy and Myosin Studies
- Electron and X-Ray Spectroscopy Techniques
- Monoclonal and Polyclonal Antibodies Research
- Characterization and Applications of Magnetic Nanoparticles
- Nuclear Structure and Function
- Advanced Biosensing Techniques and Applications
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2015-2024
Université Grenoble Alpes
2015-2024
CEA Grenoble
2015-2024
Centre National de la Recherche Scientifique
2015-2024
Laboratoire Physiologie Cellulaire & Végétale
2007-2024
Laboratoire de Physiologie Cellulaire
2015-2024
Inserm
2003-2022
Université Paris Cité
2018-2022
Hôpital Saint-Louis
2018-2019
Université Joseph Fourier
2002-2013
Eukaryotic cells rely on long-lived microtubules for intracellular transport and as compression-bearing elements. We considered that are acetylated inside their lumen microtubule acetylation may modify mechanics. Here, we found tubulin is required the mechanical stabilization of in cells. Depletion acetyltransferase TAT1 led to a significant increase frequency breakage. Nocodazole-resistant lost upon removal were largely restored by either pharmacological or physical compressive forces. In...
A number of cellular processes use both microtubules and actin filaments, but the molecular machinery linking these two cytoskeletal elements remains to be elucidated in detail. Formins are actin-binding proteins that have multiple effects on dynamics, one formin, mDia2, has been shown bind stabilize through its formin homology 2 (FH2) domain. Here we show three formins, INF2, mDia1, display important differences their interactions with actin. Constructs containing FH1, FH2, C-terminal...
Abstract Cell polarity is required for the functional specialization of many cell types including lymphocytes. A hallmark reorientation centrosome that allows repositioning organelles and vesicles in an asymmetric fashion. The mechanisms underlying polarization are not fully understood. Here we found resting lymphocytes, centrosome-associated Arp2/3 locally nucleates F-actin, which needed tethering to nucleus via LINC complex. Upon lymphocyte activation, partially depleted from as a result...
The Arabidopsis MAP65s are a protein family with similarity to the microtubule-associated proteins PRC1/Ase1p that accumulate in spindle midzone during late anaphase mammals and yeast, respectively. Here we investigate molecular functional properties of AtMAP65-5 improve our understanding AtMAP65-1 properties. We demonstrate that, vitro, both promote formation planar network antiparallel microtubules. In vivo, show selectively binds preprophase band prophase microtubule prophase, whereas...
Microtubules play a key role in cell division, motility, and intracellular trafficking. Microtubule lattices are generally regarded as stable structures that undergo turnover through dynamic instability of their ends [1Desai A. Mitchison T.J. polymerization dynamics.Annu. Rev. Cell Dev. Biol. 1997; 13: 83-117Crossref PubMed Scopus (1955) Google Scholar]. However, recent evidence suggests microtubules also exchange tubulin dimers at the sites lattice defects, which can be induced by...
Summary Higher plant cells exhibit interphase microtubule arrays specific to plants, which are essential for their developmental program. These cortical microtubules (CMT) consist of a population highly dynamic that usually organized into bundles in the cortex cells. The organization CMT is intimately linked acquisition specialized functions, and subsequentchanges distribution affect properties. mechanisms underlying formation still unclear, little known about proteins involved this...
Microtubules (MTs) are dynamic cytoskeletal elements involved in numerous cellular processes. Although they highly rigid polymers with a persistence length of 1-8 mm, may exhibit curved shape at scale few micrometers within cells, depending on their biological functions. However, how MT flexural rigidity cells is regulated remains poorly understood. Here we ask whether MT-associated proteins (MAPs) could locally control the mechanical properties MTs. We show that two major cross-linkers...
Temporal and spatial assembly of microtubules in plant cells depends mainly on the activity microtubule-interacting proteins, which either stabilize, destabilize or translocate microtubules. Recent data have revealed that thale cress (Arabidopsis thaliana) contains a protein related to p60 catalytic subunit animal katanin, microtubule-severing protein. However, effects microtubule are not known. We report first functional evidence recombinant A. thaliana katanin subunit, Atp60, binds severs...
The acentrosomal plant mitotic spindle is uniquely structured in that it lacks opposing centrosomes at its poles and equipped with a connective preprophase band regulates the spatial framework for orientation mobility. These features are supported by specialized microtubule-associated proteins motors. Here, we show Arabidopsis thaliana MAP65-4, non-motor microtubule associated protein (MAP) belongs to evolutionarily conserved MAP65 family, specifically associates forming during prophase...
Tubulins are critical for the internal organization of eukaryotic cells, and understanding their emergence is an important question in eukaryogenesis. Asgard archaea closest known prokaryotic relatives to eukaryotes. Here, we elucidated apo nucleotide-bound x-ray structures tubulin from hydrothermal living Odinarchaeota (OdinTubulin). The guanosine 5'-triphosphate (GTP)-bound structure resembles a microtubule protofilament, with GTP bound between subunits, coordinating "+" end subunit...
Active cytoskeletal materials in vitro demonstrate self-organizing properties similar to those observed their counterparts cells. However, the search emulate phenomena living matter has fallen short of producing a network that would be structurally stable yet possess adaptive plasticity. Here, we address this challenge by combining polymers composite where self-assembling microtubules and actin filaments collectively self-organize due activity microtubule-percolating molecular motors. We...
The spatial organization of the microtubule (MT) network directs cell polarity and mitosis. It is finely regulated by hundreds different types microtubule-associated proteins molecular motors whose specific functions are difficult to investigate directly in cells. Here, we have investigated their using geometrically controlled MT networks vitro cell-free assay. This was achieved developing a new method spatially define nucleation microseeds adsorbed on micropatterned glass substrate. could...
Microtubules are dynamic polymers that used for intracellular transport and chromosome segregation during cell division. Their instability stems from the low energy of tubulin dimer interactions, which sets growing polymer close to its disassembly conditions. function in coordination with kinesin dynein molecular motors, use ATP hydrolysis produce mechanical work move on microtubules. This raises possibility forces produced by walking motors can break interactions trigger microtubule...
The crosstalk between the actin network and microtubules is essential for cell polarity. It orchestrates microtubule organization within cell, driven by asymmetry of architecture along periphery. physical intertwining these networks regulates spatial force distribution in network. Although their biochemical interactions are becoming clearer, mechanical aspects remain less understood. To explore this interplay, we developed an vitro reconstitution assay to investigate how dynamic interact...
ABSTRACT The dynamic instability of microtubules is powered by the addition and removal tubulin dimers at ends microtubule. Apart from end, microtubule shaft not considered to be dynamic. However recent evidence suggests that free can incorporated into a damaged mechanical stress. Here we explored whether dimer exchange was core property lattice independently any external constraint. We found removed sites along shaft. Furthermore, showed experiment modeling rapid renewal requires structural...