A5053340415- Cellular Mechanics and Interactions
- Microtubule and mitosis dynamics
- 3D Printing in Biomedical Research
- Advanced Fluorescence Microscopy Techniques
- Protist diversity and phylogeny
- Cellular transport and secretion
- Force Microscopy Techniques and Applications
- Diabetes and associated disorders
- Spaceflight effects on biology
- Diabetes Management and Research
- Genomics and Phylogenetic Studies
- Hippo pathway signaling and YAP/TAZ
- Ubiquitin and proteasome pathways
- Micro and Nano Robotics
- Wnt/β-catenin signaling in development and cancer
- Photosynthetic Processes and Mechanisms
- Enzyme Structure and Function
- Neuroscience and Neural Engineering
- Pancreatic function and diabetes
- Bacterial Genetics and Biotechnology
Université Grenoble Alpes
2019-2025
CEA Grenoble
2019-2025
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2019-2025
Centre National de la Recherche Scientifique
2019-2025
Laboratoire Physiologie Cellulaire & Végétale
2022-2025
Laboratoire de Physiologie Cellulaire
2019-2023
Morpho (United States)
2020
Biotechnology Institute
2019
National University Cancer Institute, Singapore
2011
National University of Singapore
2011
Abstract Intracellular organization is largely mediated by actin turnover. Cellular networks continuously assemble and disassemble, while maintaining their overall appearance. This behavior, called “dynamic steady state,” allows cells to sense adapt environment. However, how structural stability can be maintained during the constant turnover of a limited monomer pool poorly understood. To answer this question, we developed an experimental system where polystyrene beads are propelled comet in...
Asgard archaea genomes contain potential eukaryotic-like genes that provide intriguing insight for the evolution of eukaryotes. The eukaryotic actin polymerization/depolymerization cycle is critical providing force and structure in many processes, including membrane remodeling. In general, encode two classes actin-regulating proteins from sequence analysis, profilins gelsolins. were demonstrated to regulate filament nucleation. Here, we identify severing, capping, annealing bundling, monomer...
WW-binding protein 2 (WBP2) has been demonstrated in different studies to be a tyrosine kinase substrate, activate estrogen receptor (ERα)/progesterone (PR) transcription, and play role breast cancer. However, the of WBP2 phosphorylation regulating ERα function cancer biology is unknown. Here, we established as target signaling via EGFR crosstalk. Using dominant-negative, constitutively active mutants, RNAi, pharmacological studies, that at Tyr192 Tyr231 could regulated by c-Src c-Yes...
In all eukaryotic cells, the actin cytoskeleton is maintained in a dynamic steady-state. Actin filaments are continuously displaced from cell periphery, where they assemble, towards cell's center, disassemble. Despite this constant flow and turnover, cellular networks maintain their overall architecture constant. How such of material can support yet steady architectures remains an open question. To investigate role myosin-based forces contractile steady-states networks, we used reconstituted...
In all living systems, the cytoplasm is separated from external environment by membranes. This confinement imposes spatial constraints on self-organization of internal components, filaments and organelles. While reconstituted systems are instrumental for understanding fundamental biological principles, traditional experiments often utilize volumes vastly larger than actual cells. recent studies, water-in-oil droplets or giant unilamellar vesicles have been widely used to impose confinement....
The shape of cells is the outcome balance inner forces produced by actomyosin network and resistive cell adhesion to their environment. specific contributions contractile, anchoring friction deformation rate orientation are difficult disentangle in living where they influence each other. Here, we reconstituted contractile networks vitro study specifically role between its substrate. To modulate magnitude spatial distribution forces, used glass or lipids surface micropatterning control...
Abstract Charting the emergence of eukaryotic traits is important for understanding characteristics organisms that contributed to eukaryogenesis. Asgard archaea and eukaryotes are only known possess regulated actin cytoskeletons. Here, we determined gelsolins (2DGels) from Lokiarchaeota (Loki) Heimdallarchaeota (Heim) capable regulating dynamics in vitro when expressed cells. The filament severing capping, monomer sequestering, functionalities 2DGels strictly calcium controlled. We X-ray...
The crosstalk between the actin network and microtubules is essential for cell polarity. It orchestrates microtubule organization within cell, driven by asymmetry of architecture along periphery. physical intertwining these networks regulates spatial force distribution in network. Although their biochemical interactions are becoming clearer, mechanical aspects remain less understood. To explore this interplay, we developed an vitro reconstitution assay to investigate how dynamic interact...
Asgard archaea genomes contain potential eukaryotic-like genes that provide intriguing insight for the evolution of eukaryotes. The actin polymerization/depolymerization cycle is critical providing force and structure a variety processes in eukaryotes, including membrane remodelling. Here, we identify filament severing, capping, annealing bundling, monomer sequestration activities by gelsolin proteins from Thorarchaeota (Thor), which complete eukaryote-like depolymerization cycle. Thor...
Co-encapsulation of pancreatic islets with mesenchymal stem cells in a 3 dimensional biomaterial's structure is promising technique to improve transplantation efficacy and decrease immunosuppressant therapy. Currently, evaluation graft quality after co-encapsulation only based on insulin secretion. Viability measurement 3D conformation involving two different cell-types complex, mainly performed manually, highly time consuming examiner dependent. Standardization encapsulated viability...
Abstract Intracellular organization is largely mediated by the actin turnover. Cellular networks consume matter and energy to sustain their dynamics, while maintaining appearance. This behavior, called ‘dynamic steady state’, enables cells sense adapt environment. However, how structural stability can be maintained during constant turnover of a limited monomer pool poorly understood. To answer this question, we developed an experimental system using bead motility in compartment with amount...
Abstract The shape of cells is the outcome balance inner forces produced by actomyosin network and resistive cell adhesion to their environment. specific contributions contractile, anchoring friction deformation rate orientation are difficult disentangle in living where they influence each other. Here, we reconstituted contractile acto-myosin networks vitro study specifically role between its substrate. To modulate magnitude spatial distribution forces, micropatterned actin nucleation...
The crosstalk between actin network and microtubules is key to the establishment of cell polarity. It ensures that asymmetry architec ture along periphery directs organization in interior. In particular, way two networks are physically inter-twined regulates spatial distribution forces microtubule network. While their biochemical getting uncovered, mechanical still poorly understood. Here we designed an vitro reconstitution assay study physical interaction dynamic with various structures...
Abstract The position of centrosome, the main microtubule-organizing center (MTOC), is instrumental in definition cell polarity. It defined by balance tension and pressure forces network microtubules (MTs). As MTs polymerize against periphery, increases produces pushing on MTOC. How mechanical interplay between actin involved regulation these remains poorly understood, particular because its investigation technically limited structural biochemical complexity cytoplasm. Here, a cell-free...