A5078788911- Microtubule and mitosis dynamics
- Photosynthetic Processes and Mechanisms
- Cellular Mechanics and Interactions
- Biopolymer Synthesis and Applications
- Protein purification and stability
- Congenital Diaphragmatic Hernia Studies
- Enzyme Structure and Function
- Advanced NMR Techniques and Applications
- Genomics and Chromatin Dynamics
- Metal Forming Simulation Techniques
- Metallurgy and Material Forming
- Ubiquitin and proteasome pathways
- Neonatal Respiratory Health Research
- Numerical methods in engineering
- Physiological and biochemical adaptations
- Neurobiology and Insect Physiology Research
- Nanoparticle-Based Drug Delivery
Utrecht University
2024
Chinese Institute for Brain Research
2023
Tsinghua University
2023
Hunan University
2023
Second Military Medical University
2013
Abstract γ-Tubulin ring complex (γ-TuRC) is the major microtubule-nucleating factor. After nucleation, microtubules can be released from γ-TuRC and stabilized by other proteins, such as CAMSAPs, but biochemical cross-talk between minus-end regulation pathways poorly understood. Here we reconstituted this process in vitro using purified components. We found that all CAMSAPs could bind to minus ends of γ-TuRC-attached microtubules. CAMSAP2 CAMSAP3, which decorate stabilize growing not tracking...
Mechanoreceptor cells develop specialized mechanosensory organelles (MOs), where force-sensitive channels and supporting structures are organized in an orderly manner to detect forces. It is intriguing how MOs formed. Here, we address this issue by studying the of fly ciliated mechanoreceptors. We show that main structure a compound cytoskeleton formed short microtubules electron-dense materials (EDMs). In knock-out mutant DCX-EMAP, nearly absent, suggesting DCX-EMAP required for formation...
Abstract The growing plus-end is a key regulatory site for microtubule dynamics. MCAK (mitotic centromere-associated kinesin), depolymerizing kinesin, an end-binding regulator of catastrophe frequency. It intriguing how specifically binds to dynamic ends. Here, we measure the kinetics using single-molecule imaging and reveal preference. entire GTP cap, including EB cap distalmost cap. Further analysis shows that strongly GTPγS microtubules, suggesting it could recognize nucleotide-dependent...
The growing plus-end is a key regulatory site for microtubule dynamics. MCAK (mitotic centromere-associated kinesin), depolymerizing kinesin, an end-binding regulator of catastrophe frequency. It intriguing how specifically binds to dynamic ends. Here, we measure the kinetics using single-molecule imaging and reveal preference. entire GTP cap, including EB cap distalmost cap. Further analysis shows that strongly GTPγS microtubules, suggesting it could recognize nucleotide-dependent feature...
The growing plus-end is a key regulatory site for microtubule dynamics. MCAK (mitotic centromere-associated kinesin), depolymerizing kinesin, an end-binding regulator of catastrophe frequency. It intriguing how specifically binds to dynamic ends. Here, we measure the kinetics using single-molecule imaging and reveal preference. entire GTP cap, including EB cap distalmost cap. Further analysis shows that strongly GTPγS microtubules, suggesting it could recognize nucleotide-dependent feature...
Aluminum alloy thin-walled structures are widely used in the automotive industry due to their advantages related light weight and crashworthiness.They can be produced at room temperature by electrohydraulic forming process.In present study, influence of parameters on quality a 6063 aluminum sinusoidal corrugation tube has been assessed.In particular, orthogonal experimental design (OED) central composite (CCD) methods have used.Through range analysis variance data, degree wire diameter (WD)...