A5063009940- Cellular Mechanics and Interactions
- Cell Adhesion Molecules Research
- Cellular transport and secretion
- Protein Kinase Regulation and GTPase Signaling
- Advanced Fluorescence Microscopy Techniques
- Microtubule and mitosis dynamics
- Cancer Cells and Metastasis
- Retinal Development and Disorders
- Receptor Mechanisms and Signaling
- Cell Image Analysis Techniques
- Hippo pathway signaling and YAP/TAZ
- Protease and Inhibitor Mechanisms
- S100 Proteins and Annexins
- 3D Printing in Biomedical Research
- Proteoglycans and glycosaminoglycans research
- Wnt/β-catenin signaling in development and cancer
- Pancreatic function and diabetes
- Monoclonal and Polyclonal Antibodies Research
- Marine animal studies overview
- Polymer Surface Interaction Studies
- Caveolin-1 and cellular processes
- Advanced biosensing and bioanalysis techniques
- Signaling Pathways in Disease
- Biotin and Related Studies
- Erythrocyte Function and Pathophysiology
Albert Einstein College of Medicine
2015-2025
Yeshiva University
2009-2021
The Bronx Defenders
2014
University of North Carolina at Chapel Hill
2004-2013
UNC Lineberger Comprehensive Cancer Center
2013
University of California, San Diego
2002-2006
Scripps Research Institute
2002-2006
Moores Cancer Center
2006
Pennsylvania State University
2000-2003
Signaling proteins are tightly regulated spatially and temporally to perform multiple functions. For Cdc42 other guanosine triphosphatases, the subcellular location of activation is a critical determinant cell behavior. However, current approaches limited in their ability examine dynamics activity living cells. We report development biosensor capable visualizing changing endogenous, unlabeled With use dye that reports protein interactions, revealed localized trans-Golgi apparatus,...
Abstract Studies of cancer cell migration have found two modes: one that is protease-independent, requiring micron-sized pores or channels for cells to squeeze through, and protease-dependent, relevant confining nanoporous matrices such as basement membranes (BMs). However, many extracellular exhibit viscoelasticity mechanical plasticity, irreversibly deforming in response force, so pore size may be malleable. Here we report the impact matrix plasticity on migration. We develop BM...
Abstract Type 2 diabetes mellitus is a major risk factor for hepatocellular carcinoma (HCC). Changes in extracellular matrix (ECM) mechanics contribute to cancer development 1,2 , and increased stiffness known promote HCC progression cirrhotic conditions 3,4 . characterized by an accumulation of advanced glycation end-products (AGEs) the ECM; however, how this affects non-cirrhotic unclear. Here we find that, patients animal models, AGEs changes collagen architecture enhance ECM...
Macrophage interactions with other cells, either locally or at distances, are imperative in both normal and pathological conditions. While soluble means of communication can transmit signals between different it does not account for all long distance macrophage interactions. Recently described tunneling nanotubes (TNTs) membranous channels that connect cells together allow transfer signals, vesicles, organelles. However, very little is known about the mechanism by which these structures...
Invadopodia are actin-rich protrusions that degrade the extracellular matrix and required for stromal invasion, intravasation, metastasis. The role of focal adhesion protein talin in regulating these structures is not known. Here, we demonstrate invadopodial degradation three-dimensional invasion metastatic breast cancer cells. sodium/hydrogen exchanger 1 (NHE-1) linked to cytoskeleton by ezrin/radixin/moesin family proteins known regulate invadopodium-mediated degradation. We show C...
Pathogen-mediated activation of macrophages arms innate immune responses that include enhanced surface ruffling and macropinocytosis for environmental sampling receptor internalization signaling. Activation with bacterial lipopolysaccharide (LPS) generates prominent dorsal ruffles, which are precursors macropinosomes. Very rapid, high-resolution imaging live lattice light sheet microscopy (LLSM) reveals new features actions redefine the process macropinosome formation closure. We offer a...
Repetitive nucleotide or amino acid sequences are often engineered into probes and biosensors to achieve functional readouts robust signal amplification. However, these repeated notoriously prone aberrant deletion degradation, impacting the ability correctly detect interpret biological functions. Here, we introduce a facile generalizable approach solve this unappreciated problem by modifying of target mRNA make them nonrepetitive but still (“synonymous”). We first demonstrated procedure...
Abstract The process of intravasation involving transendothelial migration is a key step in metastatic spread. How the triple cell complex composed macrophage, Mena over-expressing tumor and endothelial cell, called microenvironment metastasis (TMEM), facilitates not completely understood. Previous work has shown that physical contact between macrophage results formation invadopodia, actin-rich matrix degrading protrusions, important for invasion dissemination. Herein, we show...
Abstract Expression of the tumor suppressor deleted in liver cancer‐1 (DLC‐1) is lost non‐small cell lung (NSCLC) and other human carcinomas, ectopic DLC‐1 expression dramatically reduces proliferation tumorigenicity. a multi‐domain protein that includes Rho GTPase activating (RhoGAP) domain which has been hypothesized to be basis its suppressive actions. To address importance RhoGAP function suppression, we performed biochemical biological studies evaluating NSCLC. Full‐length exhibited...
Abstract The biosensors developed in the authors' laboratory have been based on different designs, each imparting specific strengths and weaknesses. Here we describe detailed protocols for application of three exemplifying designs—first, a design which an environmentally sensitive dye is used to report activation endogenous Cdc42, followed by two FRET, one using intramolecular other intermolecular FRET. differences lead need approaches imaging image analysis. Curr. Protoc. Cell Biol ....
The small guanosine triphosphatase Rab13 functions in exocytic vesicle trafficking epithelial cells. Alterations activity have been observed human cancers, yet the mechanism of activation and its role cancer progression remain unclear. In this paper, we identify DENN domain protein DENND2B as guanine nucleotide exchange factor for develop a novel Förster resonance energy transfer–based Rab biosensor to reveal by at leading edge migrating interacts with effector MICAL-L2 cell periphery,...
The initial step of metastasis is the local invasion tumor cells into surrounding tissue. Invadopodia are actin-based protrusions that mediate matrix degradation necessary for and cells. We demonstrate Rac3 GTPase critical integrating adhesion invadopodia to extracellular (ECM) with their ability degrade ECM in breast identify two pathways at important integrin activation delivery metalloproteinases: through upstream recruiter CIB1 as well downstream effector GIT1. activity, invadopodia,...