A5070332951- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- Fungal Infections and Studies
- Antifungal resistance and susceptibility
- Mycobacterium research and diagnosis
- Nuclear Structure and Function
- Mitochondrial Function and Pathology
- Advanced biosensing and bioanalysis techniques
- Glioma Diagnosis and Treatment
- Microbial Metabolic Engineering and Bioproduction
- Neuroblastoma Research and Treatments
- Single-cell and spatial transcriptomics
- RNA modifications and cancer
- Plant nutrient uptake and metabolism
- Fermentation and Sensory Analysis
- Biofuel production and bioconversion
- DNA Repair Mechanisms
- Genomics and Chromatin Dynamics
- Bioinformatics and Genomic Networks
- CRISPR and Genetic Engineering
- Fungal and yeast genetics research
- Cell Image Analysis Techniques
- Plant Molecular Biology Research
- Advanced Fluorescence Microscopy Techniques
- Phagocytosis and Immune Regulation
Vrije Universiteit Amsterdam
2020-2025
University of Amsterdam
2022-2023
Albert Einstein College of Medicine
2015-2022
Yeshiva University
2019
NCCR Chemical Biology - Visualisation and Control of Biological Processes Using Chemistry
2011
University of Genoa
2008-2010
Swiss National Science Foundation
2010
Istituti di Ricovero e Cura a Carattere Scientifico
2008
The evolutionarily conserved mRNA export receptor Mex67/NXF1 associates with mRNAs through its adaptor, Yra1/REF, allowing ribonucleoprotein (mRNP) exit nuclear pores. However, alternate adaptors should exist, since Yra1 is dispensable for in Drosophila and Caenorhabditis elegans . Here we report that Mex67 interacts directly Nab2, an essential shuttling mRNA-binding protein required export. We further show enhances the interaction between Nab2 Mex67, becomes cells overexpressing or Mex67....
Repetitive nucleotide or amino acid sequences are often engineered into probes and biosensors to achieve functional readouts robust signal amplification. However, these repeated notoriously prone aberrant deletion degradation, impacting the ability correctly detect interpret biological functions. Here, we introduce a facile generalizable approach solve this unappreciated problem by modifying of target mRNA make them nonrepetitive but still (“synonymous”). We first demonstrated procedure...
For nearly 450 million years, mycorrhizal fungi have constructed networks to collect and trade nutrient resources with plant roots1,2. Owing their dependence on host-derived carbon, these face conflicting trade-offs in building that balance construction costs against geographical coverage long-distance resource transport from roots3. How they navigate design challenges is unclear4. Here, monitor the of living networks, we built a custom-designed robot for high-throughput time-lapse imaging...
Mitochondria are dynamic organelles that must precisely control their protein composition according to cellular energy demand. Although nuclear-encoded mRNAs can be localized the mitochondrial surface, importance of this localization is unclear. As yeast switch respiratory metabolism, there an increase in fraction cytoplasm mitochondrial. Our data point change volume increasing certain surface mitochondria. We show mRNA necessary and sufficient production levels required during growth....
On activation, the GAL genes in yeast are targeted to nuclear periphery through interaction with pore complex. Here we identify two cis-acting “DNA zip codes” from GAL1-10 promoter that necessary and sufficient induce repositioning periphery. One of these codes, GRS4, is also promote clustering alleles. a lesser extent GRS5, contribute stronger expression GAL1 GAL10 by increasing fraction cells respond inducer. The molecular mechanism controlling targeting NPC distinct interchromosomal...
We describe the generation of mouse gliomas following overexpression PDGF-B in embryonic neural progenitors. Our histopathological, immunohistochemical and genome-wide expression analyses revealed a surprising uniformity among induced tumors, despite they were generated by transducing highly heterogeneous population progenitor cells known for their ability to produce all cell types central nervous system. Comparison our microarray data with published gene sets many different murine closest...
Platelet-derived growth factor B (PDGF-B) overexpression induces gliomas of different grades from murine embryonic neural progenitors. For the first time, we formally demonstrated that PDGF-B-induced neoplasms undergo progression nontumorigenic low-grade tumors toward highly malignant forms. This result, showing PDGF-B signaling alone is insufficient to confer malignancy cells, entails requirement for further molecular lesions in this process. Our results indicate one these represented by...
S. cerevisiae (or budding yeast) is an important micro-organism for sucrose-based fermentation in biotechnology. Yet, it largely unknown how yeast adapts to sucrose transitions. Sucrose can only be metabolized when the invertase or maltose machinery are expressed and we propose that Gpr1p receptor signals extracellular availability via cAMP peak adapt cells accordingly. A transition glucose gave a transient which was maximally induced sucrose. When transitioned sucrose, signalling mutants...
Single-molecule fluorescent in situ hybridization (smFISH) has emerged as a powerful technique that allows one to localize and quantify the absolute number of mRNAs single cells. In combination with immunofluorescence (IF), smFISH can be used correlate expression an mRNA protein interest Here, we provide smFISH-IF dataset S. cerevisiae. We measured cell cycle-controlled CLN2 cycle marker alpha-tubulin. The protocol describing generation is published accompanying article "Simultaneous...
In the last years, transmembrane proteoglycan NG2 has gained interest as a therapeutic target for treatment of diverse tumor types, including gliomas, because increases its expression correlate with dismal prognosis. been shown to function co-receptor PDGF ligands whose aberrant is common in gliomas. We have recently generated glioma model based on overexpression PDGF-B neural progenitors and here we investigated possible relevance during PDGF-driven gliomagenesis.The survival curves NG2-KO...
Across all kingdoms of life, gene regulatory mechanisms underlie cellular adaptation to ever-changing environments. Regulation expression adjusts protein synthesis and, in turn, growth. Messenger RNAs are key molecules the process expression. Our ability quantitatively measure mRNA single cells has improved tremendously over past decades. This revealed an unexpected coordination between steps that control life mRNA, from transcription degradation. Here, we provide overview state-of-the-art...
Mitochondria are dynamic in their size and morphology yet must also precisely control protein composition according to cellular energy demand. This is particularly complicated for mitochondria, as they coordinate gene expression from both the nuclear mitochondrial genome. We have found that cells able use this post-transcriptionally with metabolic demands of cell through enhanced mRNA localization mitochondria. As yeast switch respiratory metabolism, increase volume fraction is, ratio...
Yra1 is an mRNA export adaptor involved in biogenesis and S. cerevisiae. overexpression was recently shown to promote accumulation of DNA:RNA hybrids favoring DNA double strand breaks (DSB), cell senescence telomere shortening, via unknown mechanism. also identified at HO-induced DSB depletion causes defects repair. Previous work from our laboratory showed that ubiquitination by Tom1 important for export. Here, we found ubiquitinated the SUMO-targeted ubiquitin ligases Slx5-Slx8 implicated...