A5060679471- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- Genomics and Chromatin Dynamics
- RNA modifications and cancer
- Epigenetics and DNA Methylation
- Single-cell and spatial transcriptomics
- RNA regulation and disease
- CRISPR and Genetic Engineering
- Advanced Fluorescence Microscopy Techniques
- Chromosomal and Genetic Variations
- Molecular Biology Techniques and Applications
- Synthesis and Characterization of Heterocyclic Compounds
- Nuclear Structure and Function
- Genetics, Aging, and Longevity in Model Organisms
- T-cell and Retrovirus Studies
- Hemodynamic Monitoring and Therapy
- Galectins and Cancer Biology
- Heusler alloys: electronic and magnetic properties
- Viral Infectious Diseases and Gene Expression in Insects
- Trace Elements in Health
- Pregnancy-related medical research
- Chemical Reactions and Isotopes
- Advanced Thermoelectric Materials and Devices
- Acute Myeloid Leukemia Research
- Veterinary Practice and Education Studies
Life Science Institute
2024
Kanazawa University
2024
Albert Einstein College of Medicine
2013-2022
The Bronx Defenders
2022
Nihon University
2021
Tohoku University Hospital
2018
University of Rochester
2008-2013
Nagasaki University
1995-2009
University of Rochester Medical Center
2008
Tokyo University of Science
2006
RNA:DNA hybrids represent a non-canonical nucleic acid structure that has been associated with range of human diseases and potential transcriptional regulatory functions. Mapping in cells reveals them to have number characteristics give insights into their functions.We find occupy millions base pairs the genome. A directional sequencing approach shows RNA component hybrid be purine-rich, indicating thermodynamic contribution vivo stability. The are enriched at loci decreased DNA methylation...
Nonsense-mediated mRNA decay (NMD) is a quality control mechanism responsible for “surveying” mRNAs during translation and degrading those that harbor premature termination codon (PTC). Currently the intracellular spatial location of NMD kinetics its step in mammalian cells are under debate. To address these issues, we used single-RNA fluorescent situ hybridization (FISH) measured PTC-containing β-globin intact single after induction gene transcription. This approach preserves temporal...
Repetitive nucleotide or amino acid sequences are often engineered into probes and biosensors to achieve functional readouts robust signal amplification. However, these repeated notoriously prone aberrant deletion degradation, impacting the ability correctly detect interpret biological functions. Here, we introduce a facile generalizable approach solve this unappreciated problem by modifying of target mRNA make them nonrepetitive but still (“synonymous”). We first demonstrated procedure...
Creutzfeldt-Jakob disease (CJD) is a transmissible neurodegenerative of humans caused by an unidentified infectious agent, the prion. To determine whether there was involvement host-encoded prion protein (PrPc) in CJD development and propagation, mice heterozygous (PrP+/-) or homozygous (PrP-/-) for disrupted PrP gene were established inoculated with mouse-adapted agent. In keeping findings previous studies using other lines PrP-less scrapie agents, no PrP-/- showed any sign 460 days after...
Mammalian mRNAs lose and acquire proteins throughout their life span while undergoing processing, transport, translation, decay. How translation affects messenger RNA (mRNA)–protein interactions is largely unknown. The pioneer round of uses newly synthesized mRNA that bound by cap-binding protein 80 (CBP80)–CBP20 (also known as the complex [CBC]) at cap, poly(A)-binding N1 (PABPN1) PABPC1 poly(A) tail, and, provided biogenesis involves pre-mRNA splicing, exon junction complexes (EJCs)...
Abstract Nonsense-mediated mRNA decay (NMD) is an degradation pathway that eliminates transcripts containing premature termination codons (PTCs). Half-lives of the mRNAs PTCs demonstrate a small percent escape surveillance and do not degrade. It known whether this represents variable within cells or, alternatively population are resistant. Here we single-cell approach with bi-directional reporter, which expresses two β-globin genes or without PTC in same cell, to characterize efficiency NMD...
Abstract The pond snail Lymnaea stagnalis is an excellent model system in which to study the neuronal and molecular substrates of associative learning its consolidation into long‐term memory. Until now, presence cyclic AMP (cAMP)‐responsive element binding protein (CREB), believed be a necessary component process learned behavior that consolidated memory, has only been assumed neurons. We therefore cloned analyzed cDNA sequences homologues CREB1 CREB2 determined these mRNAs identifiable...
Summary Bacterial release factors (RFs) 1 and 2 catalyse translation termination at UAG/UAA UGA/UAA stop codons respectively. It has been shown that limiting the amount of ribosomal protein L11 affects UAG UGA differently. To understand functional interplay between RF1/RF2, we isolated 21 distinct mutations in as suppressors either temperature‐sensitive (ts) RF1/RF2 strains or read‐through mutants lacZ nonsense (UAG UGA) strains. 10 restored ts lethal growth RF1 and/or RF2 All selected...
The heritability of chromatin states through cell division is a potential contributor to the epigenetic maintenance cellular memory prior states. macroH2A histone variant has properties regulator memory, including roles controlling gene silencing and differentiation. Its mechanisms regional genomic targeting are unknown. Here, we combined in vivo imaging with biochemical approaches show that human incorporated into G1 phase cycle following DNA replication. newly retargets same large...
Hamster embryonic cells transformed in vitro with 4-nitroquinoline-1-oxide and its derivative exhibited an increased aerobic glycolysis inhibition of respiration by glucose addition the presence pyruvate (the Crab tree effect). These changes, which are known to be characteristic many malignant strains, appear correlated grade malignancy.
The CRISPR-Cas9 system can be modified to perform "epigenetic editing" by utilizing the catalytically inactive (dead) Cas9 (dCas9) recruit regulatory proteins specific genomic locations. In prior studies, epigenetic editing with multimers of transactivator VP16 and guide RNAs (gRNAs) was found cause adverse cellular responses. These side effects may confound studies inducing new properties, especially if responses are maintained through cell divisions—an property. Here, we show how distinct...
ABSTRACT Background RNA:DNA hybrids represent a non-canonical nucleic acid structure that has been associated with range of human diseases and potential transcriptional regulatory functions. Mapping in cells reveals them to have number characteristics give insights into their Results We find occupy millions base pairs the genome. A directional sequencing approach shows RNA component hybrid be purine-rich, indicating thermodynamic contribution vivo stability. The are enriched at loci...
Abstract Nonsense-mediated mRNA decay (NMD) is an degradation pathway that eliminates transcripts containing premature termination codons (PTCs). Half-lives of the mRNAs PTCs demonstrate a small percent escape surveillance and do not degrade. It known whether this represents variable within cells or, alternatively population are resistant. Here we single-cell approach with bi-directional reporter, which expresses two β-globin genes or without PTC in same cell, to characterize efficiency NMD...