A5046059487- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Liver Disease Diagnosis and Treatment
- Silk-based biomaterials and applications
- Hepatitis C virus research
- RNA Interference and Gene Delivery
- Hepatitis B Virus Studies
- RNA regulation and disease
- Insect Utilization and Effects
- Silkworms and Sericulture Research
- Fungal Infections and Studies
- Parkinson's Disease Mechanisms and Treatments
- Cancer-related molecular mechanisms research
- Advanced Photocatalysis Techniques
- Protein Kinase Regulation and GTPase Signaling
- Hydrogen's biological and therapeutic effects
- Nuclear Structure and Function
- Trace Elements in Health
- Connexins and lens biology
- Microbial metabolism and enzyme function
- Pesticide Exposure and Toxicity
- Advanced Cellulose Research Studies
- interferon and immune responses
- Sphingolipid Metabolism and Signaling
Hanyang University
2013-2024
Hanyang University Medical Center
2019-2022
University of Rochester
2010-2021
Yonsei University
1998-2020
Seoul Institute
2020
Government of the Republic of Korea
2020
Anyang University
2015
CHA University
2014
Pennsylvania State University
2005-2010
Peking University
2007
Hepatitis C virus (HCV) nonstructural protein 5A (NS5A) has been shown to antagonize numerous cellular pathways, including the antiviral interferon-α response. However, capacity of this interact with viral polymerase suggests a more direct role for NS5A in genome replication. In study, we employed two bacterially expressed, soluble derivatives probe novel functions protein. We find that bind 3′-ends HCV plus and minus strand RNAs. The high affinity binding site 3′-end RNA maps polypyrimidine...
Molecular patterns in pathogenic RNAs can be recognized by the innate immune system, and a component of this response is interferon-induced enzyme RNA-activated protein kinase (PKR). The major activators PKR have been proposed to long double-stranded RNAs. We report that with very limited secondary structures activate 5′-triphosphate–dependent fashion vitro vivo. Activation 5′-triphosphate RNA independent RIG-I enhanced treatment type 1 interferon (IFN-α). Surveillance molecular features at...
ABSTRACT Hepatitis C virus (HCV) nonstructural protein 5A (NS5A) exhibits a preference for G/U-rich RNA in vitro . Biological analysis of the NS5A RNA-binding activity and its target sites genome will be facilitated by description NS5A-RNA complex. We demonstrate that C-4 carbonyl uracil base and, inference, C-6 guanine interact with NS5A. U-rich 5 to 6 nucleotides (nt) is sufficient high-affinity binding The minimal domain consists residues 2005 2221 (referred as I-plus). This region...
Abstract The biological impact of alternative splicing is poorly understood in fungi, although recent studies have shown that these microorganisms are usually intron-rich. In this study, we re-annotated the genome C. neoformans var. using RNA-Seq data. Comparison with grubii revealed more than 99% ORF-introns same exact position two varieties whereas UTR-introns much less evolutionary conserved. We also confirmed very common , affecting nearly all expressed genes. observed specific...
Singlet oxygen is a toxic chemical but powerful oxidant, exploited in many and biological applications. However, the lifetime of singlet air under atmospheric conditions yet to be known. This has limited safe usage air, despite being strong antimicrobial agent with unique property relaxing breathable after serving its purpose. Here, we solve this long-standing problem by combining experimental theoretical research efforts; generate using photosensitizer at local source monitor time-dependent...
Abstract Demand for a cure of liver fibrosis is rising with its increasing morbidity and mortality. Therefore, it an urgent issue to investigate therapeutic candidates. Liver progresses following ‘multi-hit’ processes involving hepatic stellate cells, macrophages, hepatocytes. The NOD-like receptor protein 3 (NLRP3) inflammasome emerging as target in fibrosis. Previous studies showed that the anti-rheumatic agent auranofin inhibits NLRP3 inflammasome; thus, this study evaluates antifibrotic...
Abstract UPF1, a novel posttranscriptional regulator, regulates the abundance of transcripts, including long noncoding RNAs (lncRNAs), and thus plays an important role in cell homeostasis. In this study, we revealed that UPF1 hepatocellular carcinoma upregulated EZH2-associated lncRNA (lncRNA-HEIH ) by binding CG-rich motif, thereby regulating (HCC) tumorigenesis. UPF1-bound lncRNA-HEIH was susceptible to degradation mediated phosphorylation via SMG1 SMG5. According analysis RNA-seq public...
Abstract The stability and quality of metazoan mRNAs are under microRNA (miRNA)-mediated nonsense-mediated control. Although UPF1, a core mediator mRNA decay (NMD), mediates the target in 3′UTR-length-dependent manner, detailed mechanism remains unclear. Here, we suggest that is not mediated by nonsense but rather miRNAs downregulate via Ago-associated UPF1/SMG7. Global analyses response to UPF1 RNA interference miRNA-deficient cells reveal requires canonical miRNA targeting. destabilization...
Abstract UPF1 and LIN28A are RNA-binding proteins involved in post-transcriptional regulation stem cell differentiation. Most studies on have focused the molecular mechanisms of differentiated cells differentiation, respectively. We reveal that directly interacts with before UPF1-UPF2 complexing, thereby reducing phosphorylation inhibiting nonsense-mediated mRNA decay (NMD). identify interacting domains LIN28A; moreover, we develop a peptide impairs UPF1-LIN28A interaction augments NMD...
The photocatalytic activity and photostability of CdS quantum dot (QD) can be remarkably enhanced by hybridization with Rh-substituted layered titanate nanosheet even at very low Rh substitution rate (<1%). Mesoporous CdS-Ti(5.2-x)/6 Rhx/2O2 nanohybrids are synthesized a self-assembly exfoliated Ti(5.2-x)/6 nanosheets QDs. partial Rh(3+)/Rh(4+) ions for Ti(4+) in is quite effective enhancing an electronic coupling between hybridized components via the formation interband 4d states. A crucial...
Requiem (REQ/DPF2) was originally identified as an apoptosis-inducing protein in mouse myeloid cells and belongs to the novel Krüppel-type zinc finger d4-protein family of proteins, which includes neuro-d4 (DPF1) cer-d4 (DPF3). Interestingly, when a portion REQ messenger ribonucleic acid (mRNA) 3′ untranslated region (3′UTR), referred G8, overexpressed K562 cells, β-globin expression induced, suggesting that 3′UTR mRNA plays physiological role. Here, we present evidence 3′UTR, along with its...
Nonsense-mediated mRNA decay (NMD) is the best-characterized surveillance mechanism that degrades a premature-termination codon (PTC)-containing mRNA. During mammalian NMD, SMG1 and UPF1, key proteins in join at PTC form an SMG1-UPF1-eRF1-eRF3 (SURF) complex by binding UPF1 to eRF3 after PTC-recognition translating ribosome. Subsequently, phosphorylated UPF1-SMG1 moves onto downstream exon junction (EJC). However, cellular events induce formation increase NMD efficiency before recognition...
Human hepatocellular carcinoma (HCC) has a high mortality rate because of the dearth effective treatments. Multiple studies have shown that overexpression UPF1, key nonsense-mediated mRNA decay (NMD) factor, reduces HCC growth through various cell signaling pathways. However, mechanism by which UPF1 expression retards proliferation regulation RNA stability remains unclear. By employing variants and transcriptome analysis, we revealed variants, not UPF1-mediated NMD, tumorigenesis....
We aimed to define an optimal target population and drug-specific biomarkers that may predict dipeptidyl peptidase (DPP)-4 inhibitor responses in non-alcoholic fatty liver disease (NAFLD).An exploration study (study I) was performed using three different NAFLD models (basket design; high-fat diet [HFD], methionine choline-deficient [MCD], high-cholesterol Western [WD] models). RNA transcriptome analysis on pre-studied tissues identify could the response DPP-4 inhibitors. In validation II),...