A5001988555- DNA and Nucleic Acid Chemistry
- Cancer therapeutics and mechanisms
- Synthesis and biological activity
- Synthesis and Biological Evaluation
- Bioactive Compounds and Antitumor Agents
- RNA Interference and Gene Delivery
- Synthesis and Characterization of Heterocyclic Compounds
- Chemical Synthesis and Analysis
- Advanced biosensing and bioanalysis techniques
- RNA and protein synthesis mechanisms
- Click Chemistry and Applications
- Foot and Ankle Surgery
- Synthesis and Biological Activity
- Synthesis of Organic Compounds
- Malaria Research and Control
- Microbial Natural Products and Biosynthesis
- Lower Extremity Biomechanics and Pathologies
- Various Chemistry Research Topics
- Analytical Chemistry and Chromatography
- DNA Repair Mechanisms
- Tendon Structure and Treatment
- Diabetic Foot Ulcer Assessment and Management
- Renal and related cancers
- PI3K/AKT/mTOR signaling in cancer
- Asymmetric Synthesis and Catalysis
Seoul National University
2014-2024
Olive View-UCLA Medical Center
2023-2024
Seoul National University Bundang Hospital
2023-2024
Ronald Reagan UCLA Medical Center
2024
Georgia State University
2004-2022
Hope College
2009-2021
Severance Hospital
2015-2021
University of California, San Francisco
2021
MedStar Union Memorial Hospital
2016-2019
M J Murdock Charitable Trust
2018
Significance Some cancers, termed carcinosarcomas (CSs), have mixed cell types, with either epithelial or mesenchymal features. Sequencing the genomes of uterine and ovarian CSs demonstrated that these different types derive from a common precursor has many mutations typical cancers. In addition, we find tumors significant burden point amplification histone genes, suggesting potential role in sarcomatous transformation. Consistent this finding, expression specific gene carcinoma cells...
Nuclear magnetic resonance line narrowing was investigated by means of a rotating r-f field experiment that is analogous to free-induction decay in sample. A single crystal uranium-doped calcium fluoride used, and measurements were made at the same orientation. An effective fluorine absorption factor 5 obtained. (C.E.S.)
Leigh syndrome (LS), the most common childhood mitochondrial disorder, has characteristic clinical and neuroradiologic features. Mutations in more than 75 genes have been identified both nuclear genome, implicating a high degree of genetic heterogeneity LS. To profile these signatures understand pathophysiology LS, we recruited 64 patients from 62 families who were clinically diagnosed with LS at Seoul National University Children's Hospital. Mitochondrial analysis followed by whole-exome...
WNT10A is a signaling molecule involved in tooth development, and defects are associated with agenesis. We characterized Wnt10a null mice generated by the knockout mouse project (KOMP) six families mutations, including novel p.Arg104Cys defect, absence of EDA, EDAR, or EDARADD variations. exhibited supernumerary mandibular fourth molars, smaller molars abnormal cusp patterning root taurodontism. Wnt10a−/− incisors showed distinctive apical–lingual wedge-shaped defects. These findings spurred...
Vaccine development for the blood stages of malaria has focused on induction antibodies to parasite surface antigens, most which are highly polymorphic. An alternate strategy evolved from observations that low-density infections can induce antibody-independent immunity different strains. To test this strategy, we treated parasitized red cells rodent Plasmodium chabaudi with seco-cyclopropyl pyrrolo indole analogs. These drugs irreversibly alkylate DNA, blocking their ability replicate. After...
Abstract Fast and complete healing of a diabetic foot ulcer (DFU) is challenging due to the hostile wound environment patients. As part multimodal treatment approach, advanced dressing material using hyaluronic acid (HA) has been found be effective. However, previous studies have used HA with additional biologics, which interferes in determining true clinical effect DFU. To examine sole effectiveness DFU treatment, prospective, randomized, placebo‐controlled, single‐center study was...
A total of 24 novel 2,5-diaryl-1,3,4-oxadiazoline analogs combretastatin A-4 (CA-4, 1) were designed, synthesized, and evaluated for biological activities. The compounds represent two structural classes; the Type I class has three methoxy groups on ring II a single group ring. Biological evaluations demonstrate that multiple features control potency. Four compounds, 2-(3′-bromophenyl)-5-(3′′,4′′,5′′-trimethoxyphenyl)-2-acetyl-2,3-dihydro-1,3,4-oxadiazoline (9l),...
The β-complementary hexamer, β-d[GTACGC], to the α-sequence, α-d[CATGCG], was synthesized by phosphotriester method. non-exchangeable proton assignments were obtained using 1D- and 2D-NMR techniques, including NOE, COSY NOESY. β-strand exists as a random coil at 21°C, however, 4°C, it forms an antiparallel self-recognition duplex annealing positions 1–4. annealed α-strand, confirmation of complete detection assignment six base pair imino protons in H2O/D2O solution 21°C. 1D-NOE experiments...
The polyamide f-ImPyIm has a higher affinity for its cognate DNA than either the parent analogue, distamycin A (10-fold), or structural isomer, f-PyImIm (250-fold), respective sequence. These findings have led to formulation of two-letter "language" in which -ImPy- central pairings associate more strongly with Watson−Crick -PyPy-, -PyIm-, and -ImIm-. Herein, we further characterize f-PyImIm, report thermodynamic differences between (f-ImPyIm) -PyIm- (f-PyImIm) pairings. DNase I footprinting...
Pyrrole (Py)-imidazole (Im)-containing polyamides bind in the minor groove of DNA and can recognize specific sequences through a stacked antiparallel dimer. It has been proposed that there are two different low energy ways to form dimer these sensitive presence terminal formamido group: (i) fully overlapped stacking mode which N-terminal heterocycles stack on amide groups between at C-terminal (ii) staggered shifted by approximately one unit direction (Structure 1997, 5, 1033−1046). Two will...
Pyrrole (Py) and imidazole (Im) polyamides can be designed to target specific DNA sequences. The effect that the pyrrole arrangement, plus sequence, have on sequence specificity binding affinity has been investigated using melting (ΔTM), circular dichroism (CD), surface plasmon resonance (SPR) studies. SPR results obtained from a complete set of triheterocyclic show dramatic difference in f-ImPyIm for its cognate (Keq = 1.9 × 108 M-1) f-PyPyIm 5.9 105 M-1), which could not anticipated prior...
Structural and dynamic properties of the self-complementary decadeoxyribonucleotide d(CGCAATTGCG)2 interaction between a prototype lexitropsin, or information-reading oligopeptide, are deduced by using high-resolution 1H NMR techniques. The nonexchangeable imino proton resonances have been completely assigned two-dimensional studies. exists as right-handed B-DNA. In spectrum 1:1 complex, selective chemical shifts removal degeneracy AH2(4), AH2(5), T-CH3(6), T-CH3(7) due to anisotropy effects...
Truncation mutations in FAM83H (family with sequence similarity 83, member H) cause autosomal dominant hypocalcified amelogenesis imperfecta (ADHCAI), but little is known about function and the pathogenesis of ADHCAI. We recruited three ADHCAI families identified two novel (p.Gln457*; p.Lys639*) one previously documented (p.Q452*) disease-causing mutations. generated characterized Fam83h-knockout/lacZ-knockin mice. Surprisingly, enamel thickness, density, Knoop hardness, morphology, prism...
Radiation and genetic attenuation of Plasmodium sporozoites are two approaches for whole-organism vaccines that protect against malaria. We evaluated chemical as an alternative vaccine strategy. Sporozoites were treated with the DNA sequence-specific alkylating agent centanamycin, a compound significantly affects blood stage parasitemia transmission murine malaria also inhibits falciparum growth in vitro. Here we show treatment berghei centanamycin impaired parasite function both vitro vivo....
Many cellular structures directly imply specific biological functions. For example, normal slit diaphragm that extend from podocyte foot processes ensure the filtering function of renal glomeruli. These slits are covered by a number surface proteins, such as nephrin, podocin, podocalyxin and CD2AP. Here we report human patient presenting with congenital nephrotic syndrome, omphalocele microcoria due to two loss-of-function mutations in PODXL, which encodes podocalyxin, inherited each parent....
Supramalleolar osteotomy (SMO) is reported to be an effective treatment for varus ankle osteoarthritis by redistributing the load line within joint. Mesenchymal stem cells (MSCs) have been proposed as a new option on basis of their cartilage regeneration ability. The purpose this study was compare clinical, radiological, and second-look arthroscopic outcomes between MSC injection with marrow stimulation alone in patients who undergone SMO. In retrospective study, 62 (64 ankles) underwent...
Imidazole containing analogues 7, 10, and 17 of distamycin wherein the C-terminus contain a dimethylamino moiety have been shown to selectively bind minor groove GC-rich sequences. Accordingly, these agents were employed as vectors for delivery variety alkylating The are attached N-terminus thus providing benzoyl N-mustards (8, 15, 18 that one, two, three imidazole units, respectively) substituted acetamides 11-14. Results from ethidium displacement assay formamides mustards 15 showed calf...
One achiral seco-hydroxycyclopropylbenz[e]indolone (seco-CBI) (12) and seven seco-amino-CBI (11a−g) analogues of CC-1065 the duocarmycins were designed, synthesized evaluated for their DNA-binding anticancer properties. These compounds contain a core 2-chloroethylnaphthalene structure they do not have stereocenter. From thermal cleavage gel analyses, 11a−g 12 demonstrated similar covalent sequence specificity to adozelesin 3 racemic seco-CBI-TMI 4 binding 5'-AAAAA(865)-3' site. Continuous...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTMolecular recognition between oligopeptides and nucleic acids: influence of van der Waals contacts in determining the 3'-terminus DNA sequences read by monocationic lexitropsinsMoses. Lee, Krzysztof. Krowicki, John A. Hartley, Richard T. Pon, J. William. LownCite this: Am. Chem. Soc. 1988, 110, 11, 3641–3649Publication Date (Print):May 1, 1988Publication History Published online1 May 2002Published inissue 1...