A5041571580- Immune Cell Function and Interaction
- IL-33, ST2, and ILC Pathways
- Asthma and respiratory diseases
- Mast cells and histamine
- Glycosylation and Glycoproteins Research
- Cell Adhesion Molecules Research
- Eosinophilic Esophagitis
- Immune Response and Inflammation
- Platelet Disorders and Treatments
- Galectins and Cancer Biology
- Gut microbiota and health
- Monoclonal and Polyclonal Antibodies Research
- T-cell and B-cell Immunology
- Renal Diseases and Glomerulopathies
- Pediatric health and respiratory diseases
- Renal and related cancers
- Angiogenesis and VEGF in Cancer
- Immunotherapy and Immune Responses
- Immune cells in cancer
- Hematopoietic Stem Cell Transplantation
- Eosinophilic Disorders and Syndromes
- Immunodeficiency and Autoimmune Disorders
- Erythrocyte Function and Pathophysiology
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Lymphatic System and Diseases
University of British Columbia
2016-2025
St. Paul's Hospital
2022-2024
Canadian Society for Immunology
2023-2024
Lung Institute
2022
St. Paul's Hospital
2021-2022
University of British Columbia Hospital
2022
British Heart Foundation
2020
Centre of Biomedical Research
2009-2019
John Wiley & Sons (Germany)
2016
Medical Genetics Center
2014
Asthma is the most prevalent pediatric chronic disease and affects more than 300 million people worldwide. Recent evidence in mice has identified a "critical window" early life where gut microbial changes (dysbiosis) are influential experimental asthma. However, current research yet to establish whether these precede or involved human We compared microbiota of 319 subjects enrolled Canadian Healthy Infant Longitudinal Development (CHILD) Study, show that infants at risk asthma exhibited...
Naive CD4+ T cell differentiation into distinct subsets of helper (Th) cells is a pivotal process in the initiation adaptive immune response. Allergens predominantly stimulate Th2 cells, causing allergic inflammation. However, why allergens induce not well understood. Here we show that group 2 innate lymphoid (ILC2s) are required to mount robust response protease-allergen papain. Intranasal administration papain stimulated ILC2s and lung inflammation elevated immunoglobulin E titers. This...
Abstract Human-pluripotent-stem-cell-derived kidney cells (hPSC-KCs) have important potential for disease modelling and regeneration. Whether the hPSC-KCs can reconstitute tissue-specific phenotypes is currently unknown. Here we show that self-organize into organoids functionally recapitulate epithelial physiology, including after genome editing. In three-dimensional cultures, epiblast-stage hPSCs form spheroids surrounding hollow, amniotic-like cavities. GSK3β inhibition differentiates...
For almost 30 years, the cell-surface protein CD34 has been widely used as a marker to assist in identification and Summary isolation of hematopoietic stem cells (HSCs) progenitors preparation for bone-marrow transplantation. In addition, it increasingly help identify other tissue-specific cells, including muscle satellite epidermal precursors. Despite its utility stem-cell marker, however, function remained remarkably elusive. This is probably because: (1) subject range post-transcriptional...
Podocalyxin is a CD34-related sialomucin that expressed at high levels by podocytes, and also mesothelial cells, vascular endothelia, platelets, hematopoietic stem cells. To elucidate the function of podocalyxin, we generated podocalyxin-deficient (podxl−/−) mice homologous recombination. Null exhibit profound defects in kidney development die within 24 hours birth with anuric renal failure. Although podocytes are present glomeruli podxl−/− mice, they fail to form foot processes slit...
It is generally agreed that most colon cancers develop from adenomatous polyps, and it this fact on which screening strategies are based. Although there overwhelming evidence to link intrinsic genetic lesions with the formation of these preneoplastic lesions, recent data suggest tumor stromal environment also plays an essential role in disease. In particular, has been suggested CD34 + immature myeloid precursor cells required for development invasion. Here we have used mice conditional...
Abstract A critical event during kidney organogenesis is the differentiation of podocytes, specialized epithelial cells that filter blood plasma to form urine. Podocytes derived from human pluripotent stem (hPSC-podocytes) have recently been generated in nephron-like organoids, but developmental stage these and their capacity reveal disease mechanisms remains unclear. Here, we show hPSC-podocytes phenocopy mammalian podocytes at capillary loop (CLS), recapitulating key features...
Intestinal tumorigenesis is a result of mutations in signaling pathways that control cellular proliferation, differentiation, and survival. Mutations the Wnt/β-catenin pathway are associated with majority intestinal cancers, while dysregulation Hippo/Yes-Associated Protein (YAP) an emerging regulator tumorigenesis. In addition, these closely related play central role during regeneration. We have previously shown methylation Hippo transducer YAP by lysine methyltransferase SETD7 controls its...
Abstract Podocalyxin is a CD34-related cell surface molecule with anti-adhesive qualities. We probed tissue microarray (n = 272) linked to long-term outcome data and found that podocalyxin was highly overexpressed in distinct subset of invasive breast carcinomas 15; 6%). Univariate disease-specific (P < 0.01) multivariate regression 0.0005) analyses indicated this overexpression an independent indicator poor outcome. Forced expression perturbed junctions between MCF-7 carcinoma cells,...
MEP21 is an avian antigen specifically expressed on the surface of Myb-Ets–transformed multipotent hematopoietic precursors (MEPs) and normal thrombocytes. Using nanoelectrospray tandem mass spectrometry, we have sequenced subsequently cloned cDNA named gene thrombomucin as it encodes a 571–amino acid protein with extracellular domain typical mucin family proteoglycans. Thrombomucin distantly related to CD34, best characterized most used human stem cell marker. It also highly homologous in...