A5072433116- Asthma and respiratory diseases
- Neuroendocrine regulation and behavior
- Neuroscience of respiration and sleep
- Pediatric health and respiratory diseases
- Breastfeeding Practices and Influences
- Neonatal Respiratory Health Research
- Gut microbiota and health
- Infant Nutrition and Health
- Obesity, Physical Activity, Diet
- Air Quality and Health Impacts
- Obstructive Sleep Apnea Research
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Food Allergy and Anaphylaxis Research
- Allergic Rhinitis and Sensitization
- Sleep and related disorders
- Effects and risks of endocrine disrupting chemicals
- Noise Effects and Management
- Reproductive System and Pregnancy
- Child Nutrition and Feeding Issues
- Infant Health and Development
- Birth, Development, and Health
- Maternal Mental Health During Pregnancy and Postpartum
- Diet and metabolism studies
- Infant Development and Preterm Care
- Indoor Air Quality and Microbial Exposure
McMaster University
2015-2024
University of British Columbia
1999-2020
University of Toronto
1999-2020
Hospital for Sick Children
2020
University of Manitoba
2020
Islamic Azad University, Tehran
2020
Simon Fraser University
2020
University of Alberta
2020
SickKids Foundation
2020
3M (United States)
2020
Asthma is the most prevalent pediatric chronic disease and affects more than 300 million people worldwide. Recent evidence in mice has identified a "critical window" early life where gut microbial changes (dysbiosis) are influential experimental asthma. However, current research yet to establish whether these precede or involved human We compared microbiota of 319 subjects enrolled Canadian Healthy Infant Longitudinal Development (CHILD) Study, show that infants at risk asthma exhibited...
The effect of neonatal and infant feeding practices on childhood obesity is unclear. gut microbiome strongly influenced by has been linked to obesity.To characterize the association between breastfeeding, microbiota, risk overweight during infancy, accounting for type timing supplementary feeding.In this study a subset 1087 infants from prospective CHILD pregnancy cohort, mothers were recruited January 1, 2009, December 31, 2012. Statistical analysis was performed February 1 20, 2017.Feeding...
Background Pre-school children spend an average of two-hours daily using screens. We examined associations between screen-time on pre-school behavior data from the Canadian Healthy Infant Longitudinal Development (CHILD) study. Methods CHILD participant parents completed Child Behavior Checklist (CBCL) at five-years age. Parents reported their child's total including gaming and mobile devices. Screen-time was categorized recommended threshold two-hours/day for or one-hour/day three-years....
OBJECTIVES: Studies addressing breastfeeding and obesity rarely document the method of breast milk feeding, type supplementation, or feeding in hospital. We investigated these practices CHILD birth cohort. METHODS: Feeding was reported by mothers documented from hospital records. Weight BMI z scores (BMIzs) were measured at 12 months. Analyses controlled for maternal other confounders. RESULTS: Among 2553 mother-infant dyads, 97% initiated breastfeeding, median duration 11.0 Most infants...
The Canadian Healthy Infant Longitudinal Development (CHILD) birth cohort study recruited 3624 pregnant women, most partners and 3542 eligible offspring. We hypothesise that early life physical psychosocial environments, immunological, physiological, nutritional, hormonal metabolic influences interact with genetics influencing allergic diseases, including asthma. Environmental biological sampling, innate adaptive immune responses, gene expression, DNA methylation, gut microbiome nutrition...
The neurohypophyseal hormone oxytocin (OT) is the most potent uterotonic agent known and used to induce labor. Yet, endogenous circulating OT appears not participate in induction of As shown here, finding messenger RNA peptide uterus suggests a solution for this paradox. During gestation, rat increased more than 150-fold and, at term, exceeded hypothalamic by 70-fold. Thus, during parturition, may act primarily as local mediator hormone.
Although the steps involved in biosynthesis and secretion of neuropeptide vasopressin (AVP) have been extensively studied, factors which regulate AVP gene expression remain unknown. Therefore, we sought to determine dynamics mRNA accumulation response a strong stimulus for release, i.e. during salt imbibition ensuing period rehydration. levels were determined terms absolute amounts by novel quantitative densitometric hybridization assay, using vitro synthesized sense-strand RNA as standard...
The consumption of artificial sweeteners has increased substantially in recent decades, including among pregnant women. Animal studies suggest that exposure to utero may predispose offspring develop obesity; however, our knowledge, this never been studied humans.To determine whether maternal artificially sweetened beverages during pregnancy is associated with infant body mass index (BMI [calculated as weight kilograms divided by height meters squared]).This cohort study included 3033...
Asthma is a chronic disease of the airways affecting one in ten children Westernized countries. Recently, our group showed that specific bacterial genera early life are associated with atopy and wheezing 1-year-old children. However, little known about link between gut microbiome diagnosis asthma preschool age To determine role microbiota asthma, up to 4 years enrolled Canadian Healthy Infant Longitudinal Development (CHILD) study were classified as asthmatic (n=39) or matched healthy...
Background: Human milk contains many bioactive components that are typically studied in isolation, including bacteria. We performed an integrated analysis of human oligosaccharides and fatty acids to explore their associations with microbiota. Methods: a sub-sample 393 mothers the CHILD birth cohort. Milk was collected at 3-4 months postpartum. Microbiota analysed by 16S rRNA gene V4 sequencing. Oligosaccharides were rapid high-throughput high performance gas liquid chromatography,...
The impact of breastfeeding on respiratory health is uncertain, particularly when the mother has asthma. We examined association and wheezing in first year life.We studied 2773 infants from Canadian Healthy Infant Longitudinal Development (CHILD) birth cohort. Caregivers reported infant feeding episodes at 3, 6 12 months. Breastfeeding was classified as exclusive, partial (supplemented with formula or complementary foods) none.Overall, 21% mothers had asthma, 46% breastfed for least months...
Abstract Background It is hypothesised that complex interactions between genetic and environmental factors give rise to allergy asthma in childhood. The C anadian H ealthy I nfant L ongitudinal D evelopment ( CHILD ) study was designed explore these factors. Methods a longitudinal, general population birth cohort following infants from mid‐pregnancy age 5 years. Over this time period, biological samples, questionnaires, clinical measures data are collected. Results A total of 3624 families...
To define the minimal sequences required for expression of connexin 43 gene (cx43) in myometrial cells, we generated 5′ deletion constructs a fragment extending 1686 base pairs upstream and 162 downstream transcription start site determined their ability to drive chloramphenicol acetyltransferase reporter transfected cell lines. Our investigation revealed two cis-acting regulatory elements within this fragment. Deletion region from −102 −92 led an increase promoter activity by greater than...
Dehydration represents a strong stimulus for the secretory activity of hypothalamic vasopressinergic neurons and induces sustained rise in [Arg8]vasopressin (AVP) mRNA. We now report that, addition to change mRNA accumulation, this leads an increase size mature AVP gene transcripts. Using Northern blot analysis conjunction with internal RNA markers, we demonstrate here that 6-day period salt imbibition induced gradual from 810 maximum 930 bases. This was fully reversed by day 30 following...