A5006782649- Mast cells and histamine
- Asthma and respiratory diseases
- Parasites and Host Interactions
- Food Allergy and Anaphylaxis Research
- Polyamine Metabolism and Applications
- Immune Cell Function and Interaction
- Parasite Biology and Host Interactions
- Helminth infection and control
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Nitric Oxide and Endothelin Effects
- Neuropeptides and Animal Physiology
- Urticaria and Related Conditions
- Monoclonal and Polyclonal Antibodies Research
- Receptor Mechanisms and Signaling
- Eosinophilic Esophagitis
- Salivary Gland Disorders and Functions
- Allergic Rhinitis and Sensitization
- Pediatric health and respiratory diseases
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Neuroscience of respiration and sleep
- Immune Response and Inflammation
- Parasitic Diseases Research and Treatment
- Parasitic Infections and Diagnostics
- Stress Responses and Cortisol
- Antimicrobial Peptides and Activities
University of Alberta
2013-2024
Pulmonary Associates
2010-2023
Respiratory Clinical Trials
2023
Alberta Health Services
2021
University of Calgary
1990-2018
Heritage Medical Research Clinic
2014
AstraZeneca (Canada)
2003-2010
Anesthesiology and Surgical Oncology Research Group
2009
University of Pennsylvania
2006
Northwestern University
2005
Abstract We examined the effect of vasoactive intestinal peptide, substance P, and somatostatin on concanavalin A (1 microgram/ml)-induced lymphocyte proliferation immunoglobulin (IgA, IgM, IgG) synthesis by cells from spleens, Peyer's patches, mesenteric lymph nodes. These neuropeptides (10(-7) to 10(-12) M) modulated immune responses in a dose-dependent manner. For comparative study, were used at 10(-8) M concentration. Both peptide significantly decreased DNA (30 50%), whereas P increased...
Recent reports suggesting that the actions of certain neuroenteric peptides may be mediated in part by secretion histamine and other mast cell contents could have important implications for gastrointestinal motility secretion. However, evidence a cell-hormonal interaction is based on studies using peritoneal or cutaneous cells. Because intestinal mucosal cells (MMC) differ functionally from (PMC), we compared effects several neurotransmitters hormones two types rat. MMC hyperplasia was...
The Canadian Healthy Infant Longitudinal Development (CHILD) birth cohort study recruited 3624 pregnant women, most partners and 3542 eligible offspring. We hypothesise that early life physical psychosocial environments, immunological, physiological, nutritional, hormonal metabolic influences interact with genetics influencing allergic diseases, including asthma. Environmental biological sampling, innate adaptive immune responses, gene expression, DNA methylation, gut microbiome nutrition...
We have developed a procedure for the dispersion of mast cells from intestinal lamina propria (LP) and epithelium rats infected with nematode, Nippostrongylus brasiliensis. The dispersed are morphologically histochemically similar to mucosal (MMC) in situ distinguishable peritoneal (PMC). MMC derived LP or parasitized animals secrete histamine response specific parasite antigens as well anti-IgE. Unlike PMC, these unresponsive basic secretagogues 48/80 bee venom peptide 401. Similarly, 401...
Functional mast cells have been isolated from the lamina propria of small intestine rats infected with nematode Nippostrongylus brasiliensis. The released histamine on challenge specific antigen, anti-rat IgE, concanavalin A, and calcium ionophores but were less responsive than peritoneal (MMC) same animals. Intestinal mucosa (PMC) refractory to action basic secretagogues peptide 401 bee venom compound 48/80. anti-allergic compounds disodium cromoglycate (less or equal 10(-3) M), AH 9679...
Highly purified populations of lymphocytes were obtained from the murine intestinal mucosa using EDTA-collagenase isolation procedures in combination with discontinuous density centrifugation. Intraepithelial (IEL) separated lamina propria (LPL) and, within these two populations, fractions enriched or depleted gut granular (gGL) obtained. Using cells cytotoxic assays, it was shown that both IEL and LPL possess natural killer (NK) activity, this associated gGL. The major effector NK activity...
Abstract: Under physiological conditions, skin mast cells preferentially localize around nerves, blood vessels and hair follicles. This observation, which dates back to Paul Ehrlich, intuitively suggests that these enigmatic, multifacetted protagonists of natural immunity are functionally relevant many more aspects tissue physiology than just the generation inflammatory vasodilatory responses IgE‐dependent environmental antigens. And yet, for decades, mainstream‐mast cell research has been...
In this study, we assessed the involvement of mast cells and cell-derived mediators in enhanced epithelial permeability associated with nitric oxide synthesis inhibition. Permeability small bowel was by measuring clearance a marker (51Cr-labeled EDTA) from blood to lumen presence inhibitor, NG-nitro-L-arginine methyl ester (L-NAME). L-NAME caused very rapid (10 min) increase permeability, reaching peak values (sixfold increase) within 20 min. Two cell stabilizers, doxantrazole lodoxamide,...
Lymphoid aggregates with many of the characteristics mammalian Peyer's patches (PP) were identified in chicken intestine. These PP could not be seen naked eye at time hatching, but by 10 days age, 1 or 2 50% birds. Up to 16 wk numbers increased a maximum 5 per animal and they widely scattered intestine, apart from one that was regularly found about cm anterior ileocecal junction. As birds aged, number declined so older (58 wk) only single evident near Chicken possess: distinct...
Defensins are endogenous antimicrobial peptides stored in neutrophil granules. Here we report that a panel of defensins from human, rat, guinea pig, and rabbit neutrophils all have histamine-releasing activity, degranulating rat peritoneal mast cells with EC50 ranging 70 to 2500 nM, between 45 60% the total histamine released. The for defensin-induced secretion correlates their net basic charge at neutral pH. There is no correlation release potency. Degranulation induced by has...
Journal Article Zoonotic Potential of Giardiasis in Domestic Ruminants Get access A. Buret, Buret From the Departments Biological Sciences and Microbiology Infectious Diseases Kananaskis Center for Environmental Research, University Calgary, Alberta, Canada Search other works by this author on: Oxford Academic PubMed Google Scholar N. denHollander, denHollander P. M. Wallis, Wallis D. Befus, Befus E. Olson Reprints correspondence: Dr. Olson, Sciences, BI 055, TIN IN4. The Diseases, Volume...
Mucosal mast cells (MMC) were isolated from the intestine of Nippostrongylus brasiliensis-infected rats and then activated with Ag or anti-IgE in order to assess their metabolism arachidonic acid leukotriene (LT) C4, LTB4, prostaglandin D2 (PGD2). After challenge MMC preparations 19 +/- 1% purity five worm equivalents N. brasiliensis Ag, net formation immunoreactive LTC4, PGD2 was 58 8.3, 22 4.5, 3.4 ng/10(6) cells, respectively (mean SE, n = 7). When 56 9% by generation PGD2/10(6) 107 15,...
Abstract Bronchus‐associated and gut‐associated lymphoid tissues (BALT GALT) have both functional morphologic similarities are involved in seeding lung, gut, other mucosal sites with predominantly IgA‐containing B cells. Both types of tissue engaged the regulation controlled amplification immune responses, which vary from positive responses mucosae peripheral to local systemic tolerance. Their further involvement provision cells destined reside epithelial compartment body appears likely but...