A5039631810- Immunotherapy and Immune Responses
- Neuropeptides and Animal Physiology
- Skin Protection and Aging
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Dermatology and Skin Diseases
- Asthma and respiratory diseases
- Psoriasis: Treatment and Pathogenesis
- Antimicrobial Peptides and Activities
- RNA Interference and Gene Delivery
- Cell Adhesion Molecules Research
- melanin and skin pigmentation
- Immune Response and Inflammation
- Urticaria and Related Conditions
- Adenosine and Purinergic Signaling
- Acne and Rosacea Treatments and Effects
- Spaceflight effects on biology
- Mast cells and histamine
- Allergic Rhinitis and Sensitization
- Receptor Mechanisms and Signaling
- Monoclonal and Polyclonal Antibodies Research
- Virus-based gene therapy research
- Stress Responses and Cortisol
- Contact Dermatitis and Allergies
- Bee Products Chemical Analysis
Weill Cornell Medicine
2016-2025
Cornell University
2016-2025
Lander Institute
2025
NewYork–Presbyterian Hospital
1996-2016
Albert Einstein College of Medicine
2013
Memorial Sloan Kettering Cancer Center
1997-2010
Rockefeller University
2010
New York University
2009
New York Hospital Queens
2008
Harvard University
1988-1997
Dendritic cells (DCs) are potent antigen-presenting that play a critical role in the initiation of antitumor immune responses. In this study, we show genetic modifications murine epidermis-derived DC line and primary bone marrow–derived DCs to express model antigen β-galactosidase (βgal) can be achieved through use replication-deficient, recombinant adenovirus vector, modified capable eliciting antigen-specific, MHC-restricted CTL Importantly, using metastatic lung tumor with syngeneic colon...
A transition from a subtyping to phenotyping approach in rosacea is underway, allowing individual patient management according presenting features instead of categorization by predefined subtypes. The ROSacea COnsensus (ROSCO) 2017 recommendations further support this and align with guidance other working groups.
Spaceflight induces molecular, cellular and physiological shifts in astronauts poses myriad biomedical challenges to the human body, which are becoming increasingly relevant as more humans venture into space
The SpaceX Inspiration4 mission provided a unique opportunity to study the impact of spaceflight on human body. Biospecimen samples were collected from four crew members longitudinally before (Launch: L-92, L-44, L-3 days), during (Flight Day: FD1, FD2, FD3), and after (Return: R + 1, 45, 82, 194 days) spaceflight, spanning total 289 days across 2021-2022. collection process included venous whole blood, capillary dried blood spot cards, saliva, urine, stool, body swabs, capsule Dragon HEPA...
Abstract The anterior chamber of the eye is an immunologically privileged site in which allografts survive longer than at other body sites. In this regard, it relevant that aqueous humor (AH) inhibits lymphocyte proliferation. order to analyze AH for specific substances inhibit thymocyte proliferation, samples human AH, murine and rhesus monkey were added cultures thymocytes stimulated by IL-1 or IL-2 presence PHA. All tested had potent inhibitory activity on proliferation system. Inhibitory...
The ability of epidermal Langerhans cells to present Ag for CD4-dependent immunity is well documented, and it has been hypothesized that participate in the generation against incipient neoplasms by presentation tumor-associated situ. This study examined murine (EC) induction vivo antitumor immunity. Murine were deleted Thy-1-bearing cells, cultured 50 U/ml granulocyte-macrophage-CSF 14 18 h, pulsed with tumor fragments (TF) derived from S1509a-fibrosarcoma cells. These TF-pulsed EC injected...
Abstract: Under physiological conditions, skin mast cells preferentially localize around nerves, blood vessels and hair follicles. This observation, which dates back to Paul Ehrlich, intuitively suggests that these enigmatic, multifacetted protagonists of natural immunity are functionally relevant many more aspects tissue physiology than just the generation inflammatory vasodilatory responses IgE‐dependent environmental antigens. And yet, for decades, mainstream‐mast cell research has been...
IL-10 inhibits Langerhans cell (LC) Ag presentation to Th1 clones. As LC are capable of presenting tumor-associated Ags (TAA) for primary and secondary tumor immune responses, we examined the effect on in a model immunity S1509a spindle (H-2a). Because induction requires exposure granulocyte-macrophage (GM)-CSF, this system also allowed us study regulatory interactions GM-CSF LC. Naive CAF1 (H-2a/d) mice could be immunized against by injection with GM-CSF-exposed TAA-pulsed epidermal cells...
Subcutaneous implantation of osmotic pumps into CAF1 mice resulted in the formation thick fibrous capsules around pumps. When were loaded with recombinant murine gamma-interferon (rMuIFN-gamma) to deliver 2 X 10(3) U/h for 14 d, there was a marked decrease thickness and collagen content from rMuIFN-gamma-treated animals compared receiving diluent alone. The estimated by hydroxyproline analysis tissue quantitative electron microscopy bundles. Heat-inactivated rMuIFN-gamma failed reduce...
Interferon-gamma (IFN-gamma) suppresses the synthesis of collagen by fibroblasts in vitro and vivo animal models. Therefore, recombinant human IFN-gamma was examined for its ability to clinically modify keloids. Subjects were treated injection either 0.01 or 0.1 mg into one lesional site diluent alone another three times per week 3 weeks. Keloids measured photographed before beginning therapy weekly thereafter. Three days after final injection, biopsies performed on control sites. Six eight...
Abstract Cathelicidins are antimicrobial peptides of the innate immune system that establish an barrier at epithelial interfaces and have been proposed to a proinflammatory function. We studied role cathelicidin in allergic contact dermatitis, model requiring dendritic cells response T adaptive response. Deletion murine gene Cnlp enhanced response, whereas local administration before sensitization inhibited TLR4 but not TLR2 mediated induction cell maturation cytokine release, this...
Abstract Spaceflight can change metabolic, immunological, and biological homeostasis cause skin rashes irritation, yet the molecular basis remains unclear. To investigate impact of short-duration spaceflight on skin, we conducted biopsies Inspiration4 crew members before (L-44) after (R + 1) flight. Leveraging multi-omics assays including GeoMx™ Digital Spatial Profiler, single-cell RNA/ATAC-seq, metagenomics/metatranscriptomics, assessed spatial gene expressions associated microbial immune...
Abstract The sympathetic nervous system modulates immune function at a number of levels. Within the epidermis, APCs (Langerhans cells (LC)) are frequently anatomically associated with peripheral nerves. Furthermore, some neuropeptides have been shown to regulate LC Ag-presenting function. We explored expression adrenergic receptors (AR) in murine and assessed their functional role on Ag presentation modulation cutaneous responses. Both purified LC-like cell lines XS52-4D XS106 expressed mRNA...
Calcitonin gene-related peptide (CGRP)-containing nerves are frequently associated anatomically with epidermal Langerhans cells (LC), and LC Ag-presenting function is down-regulated by CGRP. To investigate possible regulation of cutaneous immunity CGRP, we examined its effect on the induction delayed-type hypersensitivity (DTH) contact (CHS). A system to murine spindle cell tumor S1509a (H-2a) was employed examine DTH LC. This requires exposure (EC) GM-CSF for substantial immunity. EC were...
Epidermal Langerhans cells (LC) are associated anatomically with epidermal nerves, and a product of these calcitonin gene-related peptide (CGRP), inhibits the antigen-presenting capacity LC macrophages. As CGRP receptor appears to be coupled Gs alpha protein, which in turn activates adenylate cyclase, ability induce cAMP was examined correlated functional effects. were isolated from murine using antibodies on magnetic microspheres. Exposure induced significant increase content, could...
Epidermal cell-derived thymocyte activating factor (ETAF), a cytokine produced by keratinocytes, has previously been shown to be biochemically and functionally very similar, if not identical, interleukin 1 (IL-1). Both ETAF IL-1 have demonstrated chemotactic for neutrophils mononuclear cells in vitro. In order demonstrate that this activity physiological relevance we used simple vivo model. The present study demonstrates injection of high-titer or purified recombinant murine into the mouse...