A5100780391- Global Maternal and Child Health
- Diverse Approaches in Healthcare and Education Studies
- Tuberculosis Research and Epidemiology
- Health and Wellbeing Research
- Consumer Perception and Purchasing Behavior
- Maternal and Perinatal Health Interventions
- Healthcare Education and Workforce Issues
- Education and Learning Interventions
- Adolescent Sexual and Reproductive Health
- Child Nutrition and Water Access
- Diverse Topics in Contemporary Research
- Patient-Provider Communication in Healthcare
- Healthcare Systems and Reforms
- Educational Systems and Policies
- Autophagy in Disease and Therapy
- Cultural and Historical Studies
- Attachment and Relationship Dynamics
- Maternal and fetal healthcare
- Emergency and Acute Care Studies
- Mycobacterium research and diagnosis
- Migration, Health and Trauma
- HIV/AIDS Research and Interventions
- Psychosocial Factors Impacting Youth
- Stroke Rehabilitation and Recovery
- Prenatal Screening and Diagnostics
Institut Pasteur Korea
2012-2024
Johns Hopkins University
2014-2024
Vrije Universiteit Amsterdam
2022
Nanostring Technologies (United States)
2021-2022
University of Groningen
2015-2021
Hanyang University
1997-2021
University Medical Center Groningen
2019-2021
Dongguk University
2021
Pusan National University Hospital
2021
Andong Science University
2021
Autophagy, the starvation-induced degradation of bulky cytosolic components, is up-regulated in mammalian cells when nutrient supplies are limited. Although target rapamycin (mTOR) known as key regulator autophagy induction, mechanism by which mTOR regulates has remained elusive. Here, we identify that phosphorylates a homologue Atg13 and Atg1 homologues ULK1 ULK2. The binds both ULK2 mediates interaction ULK proteins with FIP200. binding stabilizes activates facilitates phosphorylation...
Endostatin, a fragment of collagen XVIII, is potent anti-angiogenic protein, but the molecular mechanism its action not yet clear. We examined effects endostatin on biological and biochemical activities vascular endothelial growth factor (VEGF). Endostatin blocked VEGF-induced tyrosine phosphorylation KDR/Flk-1 activation ERK, p38 MAPK, p125(FAK) in human umbilical vein cells. also inhibited binding VEGF(165) to both cells purified extracellular domain KDR/Flk-1. Moreover, VEGF(121)...
ULK1 (unc-51 like autophagy activating kinase 1), the key mediator of MTORC1 signaling to autophagy, regulates early stages autophagosome formation in response starvation or inhibition. How induction process remains elusive. Here, we identify that ATG13, a binding partner ULK1, mediates interaction with ATG14-containing PIK3C3/VPS34 complex, machinery for initiation formation. The enables phosphorylate ATG14 manner dependent upon inducing conditions, such as nutrient phosphorylation mimics...
Abstract In less than nine months, the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) killed over a million people, including >25,000 in New York City (NYC) alone. The COVID-19 pandemic caused by SARS-CoV-2 highlights clinical needs to detect infection, track strain evolution, and identify biomarkers of disease course. To address these challenges, we designed fast (30-minute) colorimetric test (LAMP) for infection from naso/oropharyngeal swabs large-scale shotgun...
The protein kinase mammalian target of rapamycin (mTOR) plays an important role in the coordinate regulation cellular responses to nutritional and growth factor conditions. mTOR achieves these roles through interacting with raptor rictor form two distinct complexes, mTORC1 mTORC2. Previous studies have been focused on elucidate central complex mediating signals synthesis machinery. Functions mTORC2, relative mTORC1, remained little understood. Here we report identification a novel component...
A critical unmet clinical need to combat the global tuberculosis epidemic is development of potent agents capable reducing time multi-drug-resistant (MDR) and extensively-drug-resistant (XDR) therapy. In this paper, we report on optimization imidazo[1,2-a]pyridine amide (IPA) lead compound 1, which led design synthesis Q203 (50). We found that linker with IPA core very important for activity against Mycobacterium H37Rv. Linearity lipophilicity amine part in series play a role improving vitro...
Disrespect and abuse of women during institutional childbirth services is one the deterrents to utilization maternity care in Ethiopia other low- middle-income countries. This paper describes prevalence respectful (RMC) mistreatment hospitals health centers, identifies factors associated with occurrence RMC labor services. study had a cross sectional design. Trained external observers assessed provided 240 28 centers using structured observation checklists. The outcome variable, providers’...
[Purpose] The present study aimed to determine the correlation between activities of daily living and quality life (QOL) patients with chronic stroke. [Subjects Methods] subjects were 68 Three questionnaires distributed by visiting subjects. [Results] All items total scores functional independence measure (FIM) showed a high QOL score. relationship score significant positive correlation. Among FIM items, mobility social cognition most effects. modified r(2) value was 0.67, explained...
Abstract Background Poor job conditions and limited resources are reducing satisfaction motivation among nurses in low-income countries, which may affect the quality of services attrition rates. The objective this study was to examine satisfaction, associated factors working public health facilities Ethiopia, with aim improving performance productivity care system. Methods employed a cross-sectional two-stage cluster sampling design. From random sample 125 facilities, 424 were randomly...
Midwifery support and care led by midwives is the most appropriate strategy to improve maternal newborn health. The Government of Ethiopia has recently improved availability scaling up pre-service education. However, extent which graduating students acquire core competencies for safe effective practice not known. purpose this study was evaluate quality midwifery education assessing competence students. We conducted a cross-sectional assess who completed basic in 2013. interviewed obtain...
Pakistan has a high burden of newborn mortality, which would be significantly preventable through appropriate routine immunization. The purpose this study was to measure the basic timely childhood immunization coverage and identify determinants factors influencing in Sindh, Pakistan.Data from Maternal Child Health Program Indicator Survey 2013-2014 conducted Sindh province used. Outcome full schedule child's vaccination card. association receiving with demographic factors, socioeconomic...
Mitophagy has been implicated in mitochondrial quality control and various human diseases. However, the study of vivo mitophagy remains limited. We previously explored using a transgenic mouse expressing mitochondria-targeted fluorescent protein Keima (mt-Keima). Here, we generated mt-Keima Drosophila to extend our efforts vivo. A series experiments confirmed that can be faithfully quantitatively measured Drosophila. also showed alterations upon environmental genetic perturbation Analysis...
Prostaglandin E2(PGE2), a major product of cyclooxygenase, has been implicated in modulating angiogenesis, vascular function, and inflammatory processes, but the underlying mechanism is not clearly elucidated. We here investigated molecular by which PGE 2 regulates angiogenesis. Treatment human umbilical vein endothelial cells (HUVEC) with increased phosphorylation Akt nitric oxide synthase (eNOS), eNOS activity, (NO) production activation cAMP-dependent protein kinase (PKA)...
ULK1 (unc-51 like kinase 1) is a serine/threonine protein that plays key role in regulating the induction of autophagy. Recent studies using autophagy-defective mouse models, such as atg5- or atg7-deficient mice, revealed an important function autophagy adipocyte differentiation. Suppression adipogenesis conditions has made it difficult to study roles metabolism differentiated adipocytes. In this study, we established defective-differentiated 3T3-L1 adipocytes, and investigated Ulk1 its...
The contextual signals that regulate the expansion of prostate tumor progenitor cells are poorly defined. We found a significant fraction advanced human cancers and castration-resistant metastases express high levels β4 integrin, which binds to laminin-5. Targeted deletion signaling domain inhibited growth progression in response loss p53 Rb function mouse model cancer (PB-TAg mice). Additionally, it suppressed Pten loss-driven tumorigenesis tissue recombination experiments. traced this...
Angiogenesis is an essential step for many physiological and pathological processes. Tumor necrosis factor (TNF) superfamily cytokines are increasingly recognized as key modulators of angiogenesis. In this study, we tested whether TNF-related activation-induced cytokine (TRANCE), a new member the TNF superfamily, possesses angiogenic activity <i>in vitro</i> and<i>in vivo</i>. TRANCE stimulated DNA synthesis, chemotactic motility, capillary-like tube formation in primary cultured human...
We previously reported that endostatin inhibits endothelial and tumor cellular invasion by blocking activation catalytic activity of matrix metalloproteinase (MMP)‐2. Here we have examined the domain proMMP‐2 responsible for binding using surface plasmon resonance. ProMMP‐2 proMMP‐2ΔHP lacking hinge hemopexin‐like (HP) domains bound little to immobilized endostatin. The active MMP‐2 MMP‐2ΔHP, but not HP MMP‐2, at similar levels. In addition, preincubation MMP‐2ΔHP with MMP inhibitor...
A 1997-98 multimedia campaign promoted sexual responsibility among young people in Zimbabwe while strengthening their access to reproductive health services by training providers. Baseline and follow-up surveys each involving approximately 1400 women men aged 10-24 were conducted 5 2 comparison sites. Logistic regression analyses assess exposure the its impact on peoples knowledge discussion safer behaviors use of services. The reached 97% youth audience. Awareness contraceptive methods...
The Gambia Family Planning Association initiated a two-year IEC and Training Materials Project in 1989 with technical assistance from the Johns Hopkins University US Agency for International Development funding. projects main objectives were to improve awareness knowledge of family planning STDs increase number new continuing contraceptive users at clinics couple years protection provided through clinics. Radio materials designed convey beliefs that Islam supports use modern contraception...
A multimedia communication campaign was conducted between 1988 and 1989 to promote family planning among men in Zimbabwe. The consisted of a 52-episode semiweekly radio soap opera, about 60 motivational talks, two pamphlets contraceptive methods. Changes over time were measured by comparing subset follow-up survey from October December baseline April June 1988. Men exposed the also compared who not exposed. revealed that reached 52 percent aged 18 55. Among married Shona-speaking men, use...