A5026088399- Amyotrophic Lateral Sclerosis Research
- Tuberculosis Research and Epidemiology
- Computational Drug Discovery Methods
- Research on Leishmaniasis Studies
- vaccines and immunoinformatics approaches
- Monoclonal and Polyclonal Antibodies Research
- biodegradable polymer synthesis and properties
- Synthesis and biological activity
- Cancer therapeutics and mechanisms
- Phenothiazines and Benzothiazines Synthesis and Activities
- Pneumocystis jirovecii pneumonia detection and treatment
- HIV/AIDS drug development and treatment
- Cell death mechanisms and regulation
- Lipid metabolism and biosynthesis
- Neurogenetic and Muscular Disorders Research
- Viral gastroenteritis research and epidemiology
- Nanoparticle-Based Drug Delivery
- Platelet Disorders and Treatments
- Virus-based gene therapy research
- Plant Pathogenic Bacteria Studies
- Biotin and Related Studies
- Trypanosoma species research and implications
- Innovative Microfluidic and Catalytic Techniques Innovation
- SARS-CoV-2 and COVID-19 Research
- Plant Disease Resistance and Genetics
Institut Pasteur Korea
2011-2024
The current pipeline for new antibiotics fails to fully address the significant threat posed by drug-resistant Gram-negative bacteria that have been identified World Health Organization (WHO) as a global health priority. New antibacterials acting through novel mechanisms of action are urgently needed. We aimed identify chemical entities (NCEs) with activity against Klebsiella pneumoniae and Acinetobacter baumannii could be developed into treatment infections.
More than 100 copper/zinc superoxide dismutase 1 (SOD1) genetic mutations have been characterized. These lead to the death of motor neurons in ALS. In its native form, SOD1 protein is expressed as a homodimer cytosol. vitro studies shown that impair dimerization kinetics protein, and vivo forms aggregates patients with familial this study, we analyzed WT 9 mutant (mt) by non-invasive fluorescence techniques. Using microscopic techniques such resonance energy transfer, complementation,...
Trypanothione synthetase (TryS) catalyses the synthesis of N1,N8-bis(glutathionyl)spermidine (trypanothione), which is main low molecular mass thiol supporting several redox functions in trypanosomatids. TryS attracts attention as target for drug development against pathogens causing severe and fatal diseases mammals. A discovery campaign aimed to identify characterise new inhibitors with promising biological activity was conducted. large compound library (n = 51,624), most them bearing...
New antimalarial agents that exhibit multistage activities against drug-resistant strains of malaria parasites represent good starting points for developing next-generation therapies. To facilitate the progression such into development phase, we developed an image-based parasitological screening method defining drug effects on different asexual life cycle stages Plasmodium falciparum. High-throughput a newly assembled diversity-oriented synthetic library using this approach led to...
The massive epidemic of Ebola virus disease (EVD) in West Africa, followed recent months by two outbreaks the Democratic Republic Congo, underline importance this severe disease. Because (EBOV) must be manipulated under biosafety level 4 (BSL4) containment, discovery and development virus-specific therapies have been hampered. Recently, a transient transfection-based transcription- replication competent virus-like particle (trVLP) system was described, enabling modeling entire EBOV life...
Introduction: The identification of chemical compounds that interfere with SARS-CoV-2 replication continues to be a priority in several academic and pharmaceutical laboratories. Computational tools approaches have the power integrate, process analyze multiple data short time. However, these initiatives may yield unrealistic results if applied models are not inferred from reliable resulting predictions confirmed by experimental evidence. Methods: We undertook drug discovery campaign against...
The COVID-19 pandemic prompted several drug repositioning initiatives with the aim to rapidly deliver pharmacological candidates able reduce SARS-CoV-2 dissemination and mortality. A major issue shared by many of in silico studies addressing discovery compounds or drugs targeting molecules is that they lacked experimental validation results. Here we present a computer-aided drug-repositioning campaign against indispensable main protease (MPro 3CLPro) involved development ligand-based...
Fluorescence resonance energy transfer (FRET) and bioluminescence (BRET) are extensively used to analyze protein interactions occurring in living cells. Although these two techniques broadly applied cellular biology, comparative analysis of their strengths limitations is lacking. To this end, we analyzed a small network proteins involved the amyloidogenic processing Alzheimer β-amyloid precursor using FRET based cytometry, BRET, fluorescence lifetime imaging microscopy (FLIM). Using all...
The continuous rise of antimicrobial resistance urgently demands new therapeutic agents for human health. Drug repurposing is an attractive strategy that could significantly save time delivering antibiotics to clinics. We screened 182 US Food and Administration (FDA)-approved drugs identify potential antibiotic candidates against Staphylococcus aureus, a major pathogenic bacterium. This screening revealed the significant antibacterial activity three small molecule S. aureus: (1) LDK378...
Abstract Para -aminosalicylic acid (PAS) is an antibiotic that was largely used for the multi-therapy of tuberculosis in twentieth century. To try to overcome inconvenience its low efficacy and poor tolerance, we searched novel chemical entities able synergize with PAS using a combination screening against growing axenic Mycobacterium . The performed at sub-inhibitory concentration on library about 100,000 small molecules. Selected hit compounds were analyzed by dose–response further probed...
The extensive chemical space provides options of selecting molecules that are unique and diverse to build a truly custom made screening library. Finding the right compound from high‐throughput (HTS) could be optimized drug candidate is no simple task. Selecting meaningful compounds multitude commercially available critical; over years Institut Pasteur Korea (IPK), whose mission eliminate global threat infectious disease, had built selected library proprietary sensitive encompass structural...
Sulfur, an important component of plants, is regulated by a variety stresses in sulfate assimilation and metabolism. Increase has been observed the expression O-acetylserine(thiol)lyase (OASTL) through two-dimensional electrophoresis with shoot tips Arabidopsis infected beet severe curly top geminivirus (BSCTV). With three- to six-fold increases transcript OASTL, serine acetyltransferase (SAT) <TEX>$\gamma$</TEX>-glutmylcysteine synthetase (GSH) were induced over mock-inoculated organization...
Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease that can be caused by inherited mutations in the gene encoding copper-zinc superoxide dismutase 1 (SOD1). The structural instability of SOD1 and detection SOD1-positive inclusions familial-ALS patients supports potential causal role for misfolded and/or aggregated ALS pathology. In this study, we describe development cell-based assay designed to quantify dynamics aggregation living cells high content screening...
Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease that can be caused by inherited mutations in the gene encoding copper-zinc superoxide dismutase 1 (SOD1). The structural instability of SOD1 and detection SOD1-positive inclusions familial-ALS patients supports potential causal role for misfolded and/or aggregated ALS pathology. In this study, we describe development cell-based assay designed to quantify dynamics aggregation living cells high content screening...