A5062721339- Nuclear Receptors and Signaling
- Genetic and Kidney Cyst Diseases
- RNA Interference and Gene Delivery
- Pluripotent Stem Cells Research
- Advanced Data Storage Technologies
- Caching and Content Delivery
- Neurogenesis and neuroplasticity mechanisms
- Renal and related cancers
- CRISPR and Genetic Engineering
- Retinal Development and Disorders
- Protist diversity and phylogeny
- RNA regulation and disease
- Neuroinflammation and Neurodegeneration Mechanisms
- Spinal Cord Injury Research
- Neonatal and fetal brain pathology
- Parkinson's Disease Mechanisms and Treatments
- Genetics and Neurodevelopmental Disorders
- Telomeres, Telomerase, and Senescence
- Chromosomal and Genetic Variations
- Algorithms and Data Compression
- Dementia and Cognitive Impairment Research
- Genomics and Rare Diseases
- Nerve injury and regeneration
- Renal cell carcinoma treatment
- Hedgehog Signaling Pathway Studies
Seoul National University Hospital
2017-2024
CHA University
2012-2018
CHA Bundang Medical Center
2016-2018
Translational Research Institute
2017
Hanyang University
2007-2013
Samsung (South Korea)
2013
CHA Gangnam Medical Center
2013
Hanyang University Medical Center
2009-2010
Effective dopamine (DA) neuron differentiation from neural precursor cells (NPCs) is prerequisite for precursor/stem cell-based therapy of Parkinson's disease (PD). Nurr1, an orphan nuclear receptor, has been reported as a transcription factor that can drive DA non-dopaminergic NPCs in vitro. However, Nurr1 alone neither induces full neuronal maturation nor expression proteins found specifically midbrain neurons. In addition, inefficient inducing phenotype derived certain species such mouse...
Abstract Use of the physiological mechanisms promoting midbrain DA ( mDA ) neuron survival seems an appropriate option for developing treatments Parkinson's disease PD ). neurons are specifically marked by expression transcription factors Nurr1 and Foxa2. We show herein that Foxa2 interact to protect against various toxic insults, but their is lost during aging degenerative processes. In addition proposed cell‐autonomous actions in neurons, forced these neighboring glia synergistically...
Transplantation of neural progenitor cells (NPCs) is a potential therapy for treating neurodegenerative disorders, but this approach has faced many challenges and limited success, primarily because inhospitable host brain environments that interfere with enriched neuron engraftment function. Astrocytes play neurotrophic roles in the developing adult brain, making them candidates helping modification hostile environments. In study, we examined whether astrocytic function could be utilized to...
Abstract Neural precursor (NP) cells from adult mammalian brains can be isolated, expanded in vitro, and potentially used as cell replacement source material for treatment of intractable brain disorders. Reduced ethical concerns, lack teratoma formation, possible ex vivo autologous transplantation are critical advantages to using NP donor over fetal tissue or embryonic stem cells. However, the usage is limited by ability induce specific neurochemical phenotypes these Here, we demonstrate...
Abstract Background To date, no genetic analysis of inherited retinal disease (IRD) using whole-exome sequencing (WES) has been conducted in a large-scale Korean cohort. The aim this study was to characterise the profile IRD patients Korea WES. Methods We performed comprehensive molecular testing 168 unrelated potential pathogenicity candidate variants assessed American College Medical Genetics and Genomics Association for Molecular Pathology variant interpretation guidelines, silico...
Neonatal asphyxia is an important contributor to cerebral palsy (CP), for which there no effective treatment date. The administration of human cord blood cells (hUCBCs) emerging as a therapeutic strategy the neurological disorders. However, are few studies on application hUCBCs neonatal ischemia model CP. Experiments and behavioral tests (mainly motor tests) performed hypoxia/ischemia have been limited short-term effects hUCBCs, but mechanisms action not investigated. We study use in rat...
Parkinson’s disease (PD) is the second most common age-related neurodegenerative in elderly and patients suffer from uncontrolled movement disorders due to loss of dopaminergic (DA) neurons on substantia nigra pars compacta (SNpc). We previously reported that transplantation human fetal midbrain-derived neural precursor cells restored functional deficits a 6-hydroxy dopamine (6-OHDA)-treated rodent model PD but its low viability ethical issues still remain be solved. Albeit immune privilege...
Deciphering the molecular basis of neuronal cell death is a central issue in etiology neurodegenerative diseases, such as Parkinson's and Alzheimer's. Dysregulation p53 levels has been implicated apoptosis. The role histone deacetylase 3 (HDAC3) suppressing p53-dependent apoptosis recently emphasized; however, modulation function by HDAC3 remains unclear. Here we show that PTEN-induced putative kinase 1 (PINK1), which linked to autosomal recessive early-onset familial disease, phosphorylates...
For the last years, a number of flash translation layers (FTL) have been proposed for hiding erase-before-write architecture NAND memory. However, although many conventional FTLs efficiently provide logical to physical address remapping algorithms, they could not escape from performance degradation when handling hot data which tends generate so overwrites on same address. In this paper, we propose novel FTL algorithm called Hybrid Flash Translation Layer (HFTL) that adaptively exploits...
Abstract Nurr1 is a transcription factor specific for the development and maintenance of midbrain dopamine (DA) neurons. Exogenous in neural precursor (NP) cells induces differentiation DA neurons vitro that are capable reversing motor dysfunctions rodent model Parkinson disease. The promise this therapeutic approach, however, unclear due to poor cell survival phenotype loss after transplantation. We herein demonstrate proteins undergo ubiquitin-proteasome-system-mediated degradation...
The transplantation of human umbilical cord blood cells (hUCBCs) has been shown to attenuate the unregulated activation microglia in a rat model cerebral palsy (CP). To investigate whether hUCBCs is also anti-inflammatory humans, we performed clinical trial patients with CP.Allogeneic or autologous and erythropoietin (EPO) were intravenously injected into CP (mean age approximately 38 weeks), analyzed for their motor function social behavior. Blood samples tested cytokine levels. most...
Stem cell-based cell replacement of lost midbrain dopamine (mDA) neurons is a potential therapy for Parkinson's disease (PD). Toward this goal, it critical to optimize various aspects transplantation and assess functional recovery through behavioral tests in validated animal model(s) PD. At present, studies are being done almost exclusively neurotoxin-based models, because few genetic models PD exhibit robust mDA neuronal loss. Here we used model PD, the aphakia mouse, which demonstrates...
Abstract For the best results in quantitative polymerase chain reaction (qPCR) experiments, it is essential to design high-quality primers considering a multitude of constraints and purpose experiments. The include many filtering constraints, homology test on huge number off-target sequences, same for batch primers, exon spanning, avoiding single nucleotide polymorphism (SNP) sites. target sequences are either database or given as FASTA experiment amplifying each sequence with corresponding...
Background Oxidative stress induced by chronic hyperglycemia is recognized as a significant mechanistic contributor to the development of diabetic kidney disease (DKD). Nonphagocytic nicotinamide adenine dinucleotide phosphate oxidase 4 (Nox4) major source reactive oxygen species (ROS) in many cell types and tissue animals. We designed this study explore therapeutic potential chloroquine (CQ) amodiaquine (AQ) for inhibiting mitochondrial Nox4 tubular injury. Methods Human renal proximal...
Nurr1 is an orphan nuclear receptor‐type transcription factor (TF) that plays critical roles in midbrain dopamine neuron development. This study demonstrated a novel role for neuronal/astrocytic differentiation of neural precursor (NP) cells isolated from rat embryonic cortices: overexpression this TF promoted NP cell towards neurons at the expense astrocytic differentiation. Single cell‐based lineage analyses and experiments using co‐cultures revealed elicited its neurogenic extrinsic...
Abstract We have developed a good manufacturing practice for long-term cultivation of fetal human midbrain-derived neural progenitor cells. The generation dopaminergic neurons may serve as tool either restorative cell therapies or cellular models, particularly reference phenotyping region-specific stem lines such embryonic cells and inducible pluripotent cultivated 3 different midbrain at 10, 12, 14 weeks gestation more than year characterized them in great detail, well comparison with Lund...
Tuberous sclerosis complex–associated renal cell carcinoma (TSC-RCC) has distinct clinical and histopathologic features is considered a specific subtype of RCC. The genetic alterations TSC1 or TSC2 are responsible for the development TSC. In this study, we assessed mTOR pathway activation aimed to evaluate molecular characteristics pathogenic pathways TSC-RCC. Two cases TSC-RCC, one from 31-year-old female other an 8-year-old male, were assessed. was determined by immunohistochemistry....
Abstract Autosomal dominant polycystic kidney disease (ADPKD) is one of the main causes end-stage renal (ESRD). Genetic information utmost importance in understanding pathogenesis ADPKD. Therefore, this study aimed to demonstrate genetic characteristics ADPKD and their effects on function 749 Korean subjects from 524 unrelated families. studies PKD1/2 were performed using targeted exome sequencing combined with Sanger exon 1 PKD1 gene a multiple ligation probe assay. The mutation detection...