A5008787917- Pluripotent Stem Cells Research
- Extracellular vesicles in disease
- Genetic Neurodegenerative Diseases
- CRISPR and Genetic Engineering
- Mitochondrial Function and Pathology
- Ubiquitin and proteasome pathways
- MicroRNA in disease regulation
- Nuclear Receptors and Signaling
- Neurogenesis and neuroplasticity mechanisms
- RNA Interference and Gene Delivery
- 3D Printing in Biomedical Research
- Nerve injury and regeneration
- Amyotrophic Lateral Sclerosis Research
- RNA modifications and cancer
- Cell Image Analysis Techniques
- Genetics and Neurodevelopmental Disorders
- Skin Protection and Aging
- Advanced biosensing and bioanalysis techniques
- RNA regulation and disease
- Signaling Pathways in Disease
- Nanopore and Nanochannel Transport Studies
- Neuroscience and Neural Engineering
- Erythropoietin and Anemia Treatment
- Circular RNAs in diseases
- Acute Ischemic Stroke Management
Korea University
2016-2025
University of Bahrain
2015
McLean Hospital
2006-2013
Harvard University
2006-2013
Harvard Stem Cell Institute
2009
Harvard University Press
2008
Lung cancer has a high mortality rate, but an early diagnosis can contribute to favorable prognosis. A liquid biopsy that captures and detects tumor-related biomarkers in body fluids great potential for early-stage diagnosis. Exosomes, nanosized extracellular vesicles found blood, have been proposed as promising biopsy. Here, we demonstrate accurate of lung cancer, using deep learning-based surface-enhanced Raman spectroscopy (SERS) the exosomes. Our approach was explore features cell...
Owing to the role of exosome as a cargo for intercellular communication, especially in cancer metastasis, evidence has been consistently accumulated that exosomes can be used noninvasive indicator cancer. Consequently, several studies applying have proposed diagnostic methods such ELISA assay. However, it still challenging get reliable results due requirement labeling process and high concentration exosome. Here, we demonstrate label-free highly sensitive classification method by combining...
Exosomes, which are nanovesicles secreted by cells, promising biomarkers for cancer diagnosis and prognosis, based on their specific surface protein compositions. Here, we demonstrate the correlation of nonsmall cell lung (NSCLC) cell-derived exosomes potential markers unique Raman scattering profiles principal component analysis (PCA) diagnosis. On basis enhanced (SERS) signals from normal NSCLC extracted patterns cancerous PCA clarified as peaks through quantitative with ratiometric...
Given the therapeutic potential offered by embryonic stem (ES) cells, it is critical to optimize stable gene delivery and expression at different developmental stages of ES cell differentiation. Here, we systematically analyzed lentiviral vectors containing following promoters: human elongation factor 1α (EF1α) promoter, cytomegalovirus (CMV) immediate early region enhancer-promoter, composite CAG promoter (consisting CMV enhancer chicken β-actin promoter), phosphoglycerate kinase 1 (PGK)...
Neuropathic pain following spinal cord injury (SCI) is a devastating disease characterized by spontaneous such as hyperalgesia and allodynia. In this study, we investigated the therapeutic potential of ESC-derived GABAergic neurons to treat neuropathic in SCI rat model. Mouse embryonic stem cell-derived neural precursor cells (mESC-NPCs) were cultured media supplemented with sonic hedgehog (SHH) retinoic acid (RA) efficiently differentiated into neurons. Interestingly, low doses SHH RA...
Abstract Human embryonic stem (hES) cells have the ability to renew themselves and differentiate into multiple cell types upon exposure appropriate signals. In particular, of hES defined neural lineages, such as neurons, astrocytes, oligodendrocytes, is fundamental developing cell‐based therapies for neurodegenerative disorders studying developmental mechanisms. However, utilization basic applied research hampered by lack well‐defined methods maintain their self‐renewal direct...
Abstract Transplantation of mouse embryonic stem (mES) cells can restore function in Parkinson disease models, but generate teratomas. Purification dopamine neurons derived from by fluorescence-activated cell sorting (FACS) could provide a functional population for transplantation while eliminating the risk teratoma formation. Here we used tyrosine hydroxylase (TH) promoter to drive enhanced green fluorescent protein (eGFP) expression mES cells. First, evaluated 2.5-kilobase (kb) and 9-kb TH...
Midbrain dopamine (mDA) neurons play critical roles in the regulation of voluntary movement and their dysfunction is associated with Parkinson's disease. Pitx3 has been implicated proper development mDA substantia nigra pars compacta, which are selectively lost However, basic mechanisms underlying its role neuron and/or survival poorly understood. Toward this goal, we sought to identify downstream target genes by comparing gene expression profiles wild-type Pitx3-deficient aphakia mice. This...
Extracellular vesicles (EVs) have garnered significant attention due to their potential applications in disease diagnostics and management. However, the process of isolating EVs, primarily from blood samples, is still suboptimal. This mainly attributed abundant nature soluble proteins lipoproteins, which are often separated together with EVs end products conventional isolation methods. As such, we devise a single-step charge-based EV method by utilizing positively charged beads selectively...
Artificial intelligence (AI) is rapidly advancing, aiming to mimic human cognitive abilities, and addressing complex medical challenges in the field of biological science. Over past decade, AI has experienced exponential growth proven its effectiveness processing massive datasets optimizing decision-making. The main content this review paper emphasizes active utilization stem cells. Stem cell therapies use diverse cells for drug development, disease modeling, treatment research. However,...
To elucidate detailed functional mechanisms of key fate-determining transcription factors (eg, Nurr1, Pitx3, and Lmx1a) their interplay for midbrain dopamine (mDA) neurons, we developed highly efficient gain-of-function system by transducing the neural progenitors (NPs) derived from embryonic stem cells (ESCs) with retroviral vectors, allowing analysis downstream molecular cellular effects. Overexpression each factors, Lmx1a robustly promoted dopaminergic differentiation ESC-NP exposed to...
Abstract The generation of human oligodendrocyte progenitor cells (OPCs) may be therapeutically valuable for demyelinating diseases such as multiple sclerosis. Here, we report the direct reprogramming somatic into expandable induced OPCs (iOPCs) using a combination OCT4 and small molecule cocktail. This method enables A2B5 + (an early marker OPCs) iOPCs within 2 weeks retaining ability to differentiate MBP-positive mature oligodendrocytes. RNA-seq analysis revealed that transcriptome O4 was...
Abstract Atopic dermatitis (AD) is a chronic inflammatory skin disorder characterized by disrupted epidermal barrier function and excessive immune activation. Conventional treatments using corticosteroids immunosuppressants provide only temporary relief often induce adverse side effects, highlighting the need for novel, effective therapy. In this study, we investigated therapeutic potential of neural stem cell-extracellular vesicles (NSC-EVs) derived from NSC-conditioned medium (NSC-CM) in...
Spinocerebellar ataxia type 1 (SCA1) is an autosomal-dominant neurodegenerative disorder caused by expansion of the polyglutamine tract in SCA1 gene product, ataxin-1. Using d2EGFP, a short-lived enhanced green fluorescent protein, we investigated whether polyglutamine-expanded ataxin-1 affects function proteasome, cellular multicatalytic protease that degrades most misfolded proteins and regulatory proteins. In Western blot analysis immunofluorescence experiments, d2EGFP was less degraded...
Exosomes, derived from chronic myelogenous leukaemia (CML) cells, can be used as biomarkers and new targets for the detection of BCR-ABL transcript. This study aimed to identify these possibilities.Human CML cell line-derived exosomes CML-patients-derived were isolated with a size-exclusion chromatography column ExoQuick™ exosome precipitation solution, respectively. Isolated analysed by nested PCR detect transcript.Exosomes two human lines yielded 250-bp band. RNA sequence analysis revealed...
Stem cell-based cell replacement of lost midbrain dopamine (mDA) neurons is a potential therapy for Parkinson's disease (PD). Toward this goal, it critical to optimize various aspects transplantation and assess functional recovery through behavioral tests in validated animal model(s) PD. At present, studies are being done almost exclusively neurotoxin-based models, because few genetic models PD exhibit robust mDA neuronal loss. Here we used model PD, the aphakia mouse, which demonstrates...