A5100783457- RNA Research and Splicing
- Endoplasmic Reticulum Stress and Disease
- RNA regulation and disease
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Barrier Structure and Function Studies
- Ubiquitin and proteasome pathways
- Advanced Malware Detection Techniques
- Neurological Disease Mechanisms and Treatments
- Cancer, Stress, Anesthesia, and Immune Response
- Gene Regulatory Network Analysis
- Network Security and Intrusion Detection
- Cancer-related molecular mechanisms research
- Cancer, Hypoxia, and Metabolism
- Circular RNAs in diseases
- Single-cell and spatial transcriptomics
- Epigenetics and DNA Methylation
- CRISPR and Genetic Engineering
- Neuroinflammation and Neurodegeneration Mechanisms
- Photoreceptor and optogenetics research
- MicroRNA in disease regulation
- Cancer Cells and Metastasis
- Cardiac Fibrosis and Remodeling
- Kruppel-like factors research
- Fungal and yeast genetics research
Nanyang Institute of Technology
2020-2025
Shanghai East Hospital
2024-2025
Albert Einstein College of Medicine
2020-2024
Beijing Haidian Hospital
2024
Uppsala University
2022-2024
Shanghai Electric (China)
2024
Tongji University
2024
Renji Hospital
2024
Shanghai Jiao Tong University
2024
The Bronx Defenders
2022
Stochastic fluctuations in gene expression ("noise") are often considered detrimental, but can also be exploited for benefit (e.g., dither). We show here that DNA base excision repair amplifies transcriptional noise to facilitate cellular reprogramming. Specifically, the protein Apex1, which recognizes both naturally occurring and unnatural modifications, while homeostatically maintaining mean levels. This amplified originates from shorter-duration, higher-intensity bursts generated by...
Abstract Stress-induced condensation of mRNA and proteins into stress granules is conserved across eukaryotes, yet the function, formation mechanisms, relation to well-studied transcriptional responses remain largely unresolved. exposure ribosome-free following translational shutoff thought cause by allowing new multivalent RNA-dependent interactions, with RNA length associated interaction capacity driving increased condensation. Here we show that, in striking contrast, virtually all species...
The colon and rectum are highly innervated, with neural components within the tumor microenvironment playing a significant role in colorectal cancer (CRC) progression. While perineural invasion (PNI) is associated poor prognosis CRC, impact of nerve density diameter on behavior remains unclear. This study aims to evaluate prognostic value characteristics CRC verify nerves growth. Tissue samples from 129 patients were stained immunofluorescent markers NF-L S100B detect nerves. Nerve measured...
Abstract Perineural invasion (PNI), characterized by tumor cells surrounding and invading nerves, is associated with poor prognosis in colorectal cancer (CRC). Understanding the mechanisms of PNI crucial for developing targeted therapies to impede progression. In this study, clinical information transcriptome data are obtained from TCGA database. Stable MAGEA6 knockdown CRC cell lines established investigate impact on malignancy. Immunohistochemical staining used assess significance MAGEA6....
Abstract Inositol requiring enzyme 1 (IRE1) mitigates endoplasmic-reticulum (ER) stress by orchestrating the unfolded-protein response (UPR). IRE1 spans ER membrane, and signals through a cytosolic kinase-endoribonuclease module. The endoribonuclease generates transcription factor XBP1s intron excision between similar RNA stem-loop endomotifs, depletes select cellular mRNAs regulated IRE1-dependent decay (RIDD). Paradoxically, in mammals RIDD seems to target only with XBP1-like while flies...
Claudin-5 (CLDN5) is an endothelial tight junction protein essential for blood-brain barrier (BBB) formation. Abnormal CLDN5 expression common in brain disease, and knockdown of Cldn5 at the BBB has been proposed to facilitate drug delivery brain. To study consequences loss mature brain, we induced mosaic endothelial-specific gene ablation adult mice (Cldn5iECKO). These displayed increased permeability tracers up 10 kDa size from 6 days post induction (dpi) ensuing lethality dpi. Single-cell...
Studying disease-related changes in the brain vasculature is warranted due to its crucial role supplying oxygen and nutrients removing waste anticipated vascular dysfunction diseases. To this end, we have developed a protocol for fast simple isolation of fragments without use transgenic reporters. We used it isolate analyze 22,515 cells by single-cell RNA sequencing. The distributed into 23 distinct clusters corresponding all known perivascular cell types brain. Western blot analysis also...
The Warburg effect, also known as aerobic glycolysis, plays a crucial role in the onset and progression of colorectal cancer (CRC), although its mechanism remains unclear. In this study, bioinformatics analysis public databases combined with validation using clinical specimens identified transcription factor ONECUT3 key regulator related to effect CRC. Functionally, silencing reverses suppresses tumor growth. Importantly, promotes growth glycolysis-dependent manner through hypoxia-inducible...
During cell-cycle progression, substrates of a single master regulatory enzyme can be modified in specific order. Here, we used experimental and computational approaches to dissect the quantitative mechanisms underlying ordered degradation ubiquitin ligase APC/CCdc20, key regulator chromosome segregation mitosis. We show experimentally that rate catalysis varies with different APC/CCdc20. Using model based on multi-step ubiquitination, then how changes interaction between substrate...
The endoplasmic reticulum (ER) protein folding capacity is balanced with the burden to prevent accumulation of un- or misfolded proteins. ER membrane-resident kinase/RNase Ire1 maintains homeostasis through two fundamentally distinct processes. First, can initiate a transcriptional response non-conventional mRNA splicing reaction increase capacity. Second, decrease selective decay. In Saccharomyces cerevisiae and Schizosaccharomyces pombe, functions have been evolutionarily separated. Here,...
Liquid-liquid phase separation (LLPS) within the nucleus is directly linked to driving gene expression through transcriptional complexes. Histone lysine methyltransferase 2D (KMT2D) widely present in many cancers. It known epigenetically stimulate of genes associated with tumorigenesis and metastasis. Our analyses show that KMT2D possesses two distinct low-complexity domains (LCDs) capable assembly membrane-less condensates. The dependence mechanisms underlying monomethylation H3K4 on LLPS...
ABSTRACT Eukaryotic IRE1 mitigates endoplasmic-reticulum (ER) stress by orchestrating the unfolded-protein response (UPR). spans ER membrane, and signals through a cytosolic kinase-endoribonuclease module. The endoribonuclease generates transcription factor XBP1s intron excision between similar RNA stem-loop endomotifs, depletes select cellular mRNAs regulated IRE1-dependent decay (RIDD). Paradoxically, mammalian RIDD seemingly targets only with XBP1-like while in flies exhibits little...
The unfolded protein response (UPR) maintains folding homeostasis in the endoplasmic reticulum (ER). In metazoan cells, Ire1 branch of UPR initiates two functional outputs—non-conventional mRNA splicing and selective decay (RIDD). By contrast, orthologs from Saccharomyces cerevisiae Schizosaccharomyces pombe are specialized for only or RIDD, respectively. Previously, we showed that specialization lies Ire1’s RNase activity, which is either stringently splice-site specific promiscuous (Li et...
Cardiac fibrosis (CF) is regulated by multiple factors, including transforming growth factor β1 (TGFβ1) and noncoding RNAs.Thrombospondin 1 (TSP1) a physiologic regulator of TGFβ activation.Here, we performed microarray analyses on mRNAs lncRNAs differentially-expressed in the CF normal rat hearts.KEGG signaling annotation GO enrichment were to validate roles extracellular matrix (ECM) TSP1-enhanced activation CF.The co-expression network between ECM-related factors was constructed identify...
Abstract We aimed to investigate the effects of Krüppel‐like factor 5 (KLF5) on cell biological function and chemotherapy sensitivity anaplastic thyroid carcinoma (ATC) explore underlying mechanism. In this study, we found that KLF5 was expressed higher in ATC cells than normal cells. Knockdown inhibited proliferation, induced apoptosis restrained invasion migration abilities overexpression promoted proliferation response doxorubicin (Dox), whereas knockdown increased Dox. Multidrug...