A5006046186- Genomics and Chromatin Dynamics
- RNA Research and Splicing
- CRISPR and Genetic Engineering
- Biotin and Related Studies
- Nuclear Structure and Function
- Mitochondrial Function and Pathology
- Advanced Fluorescence Microscopy Techniques
- Click Chemistry and Applications
- RNA and protein synthesis mechanisms
- Advanced Biosensing Techniques and Applications
- Receptor Mechanisms and Signaling
- Medical Education and Admissions
- Parkinson's Disease Mechanisms and Treatments
- Genetics, Aging, and Longevity in Model Organisms
- Cellular transport and secretion
- Neurological diseases and metabolism
- Gene Regulatory Network Analysis
- Genetics and Neurodevelopmental Disorders
- Diversity and Career in Medicine
- Fungal and yeast genetics research
- Genetic Neurodegenerative Diseases
- Autophagy in Disease and Therapy
- Epigenetics and DNA Methylation
- Hereditary Neurological Disorders
- Innovations in Medical Education
Northwestern University
2015-2024
Northwestern Memorial Hospital
2023
Stanford University
2017-2021
In yeast and humans, previous experiences can lead to epigenetic transcriptional memory: repressed genes that exhibit mitotically heritable changes in chromatin structure promoter recruitment of poised RNA polymerase II preinitiation complex (RNAPII PIC), which enhances future reactivation. Here, we show INO1 memory is initiated by binding the Sfl1 transcription factor cis-acting Memory Recruitment Sequence, targeting nuclear periphery. requires a remodeled form Set1/COMPASS...
Transcriptional assays, such as yeast two-hybrid and TANGO, that convert transient protein-protein interactions (PPIs) into stable expression of transgenes are powerful tools for PPI discovery, screens, analysis cell populations. However, assays often have high background lose information about dynamics. We developed SPARK (Specific Protein Association tool giving transcriptional Readout with rapid Kinetics), in which proteolytic release a membrane-tethered transcription factor (TF) requires...
In budding yeast, targeting of active genes to the nuclear pore complex (NPC) and interchromosomal clustering is mediated by transcription factor (TF) binding sites in gene promoters. For example, for TFs Put3, Ste12, Gcn4 are necessary sufficient promote positioning at periphery clustering. However, all three cases, regulated. Under uninducing conditions, local recruitment Rpd3(L) histone deacetylase transcriptional repressors blocks Put3 DNA binding. This a general function yeast...
On activation, the GAL genes in yeast are targeted to nuclear periphery through interaction with pore complex. Here we identify two cis-acting “DNA zip codes” from GAL1-10 promoter that necessary and sufficient induce repositioning periphery. One of these codes, GRS4, is also promote clustering alleles. a lesser extent GRS5, contribute stronger expression GAL1 GAL10 by increasing fraction cells respond inducer. The molecular mechanism controlling targeting NPC distinct interchromosomal...
The trafficking of specific protein cohorts to correct subcellular locations at times is essential for every signaling and regulatory process in biology. Gene perturbation screens could provide a powerful approach probe the molecular mechanisms trafficking, but only if localization or mislocalization can be tied simple robust phenotype cell selection, such as proliferation fluorescence-activated sorting (FACS). To empower study processes with gene perturbation, we developed genetically...
Many genes localize at the nuclear periphery through physical interaction with pore complex (NPC). We have found that yeast INO1 gene is targeted to NPC both upon activation and for several generations after repression, a phenomenon called epigenetic transcriptional memory. Targeting of requires distinct cis-acting promoter DNA zip codes under activating conditions memory conditions. When periphery, active clusters itself other share GRS I code. Here, we show during memory, two alleles...
With the incorporation of pass/fail outcomes into curricula many medical schools, a greater premium is being placed on leadership, research, and other extracurricular pursuits. These activities, as well cultivation social capital, represent "hidden curriculum" which offers significant benefits to career development that are not often explicitly stated. The hidden curriculum students with generational knowledge school infrastructure harms first-generation and/or low-income (FGLI) students,...
Abstract Transcriptional assays such as yeast two hybrid, split ubiquitin, and Tango that convert transient protein-protein interactions (PPIs) in cells into stable expression of transgenes are powerful tools for PPI discovery, high-throughput screens, analysis large cell populations. However, these frequently suffer from high background they lose all information about dynamics. To address limitations, we developed a light-gated transcriptional assay detection called PPI-FLARE (PPI-Fast...
Abstract The trafficking of specific protein cohorts to the correct subcellular location at time is essential for every signaling and regulatory process in biology. Gene perturbation screens could provide a powerful approach probe molecular mechanisms trafficking, but only if localization or mislocalization can be tied simple robust phenotype cell selection, such as proliferation FACS. To broadly empower study processes with gene perturbation, we developed genetically-encoded tool named...