A5018249439- Sarcoma Diagnosis and Treatment
- Cancer Immunotherapy and Biomarkers
- Soft tissue tumor case studies
- Cancer Genomics and Diagnostics
- Soft tissue tumors and treatment
- Musculoskeletal synovial abnormalities and treatments
- Tumors and Oncological Cases
- Immune cells in cancer
- Cardiac tumors and thrombi
- Bone Tumor Diagnosis and Treatments
- Vascular Tumors and Angiosarcomas
- Immunotherapy and Immune Responses
- Pancreatic and Hepatic Oncology Research
- Muscle Physiology and Disorders
- Single-cell and spatial transcriptomics
- Lymphoma Diagnosis and Treatment
- Ferroptosis and cancer prognosis
- Cell Image Analysis Techniques
- Radiomics and Machine Learning in Medical Imaging
- Cancer-related molecular mechanisms research
- Tendon Structure and Treatment
- CAR-T cell therapy research
- Liver Disease Diagnosis and Treatment
- Gastrointestinal Tumor Research and Treatment
- Telomeres, Telomerase, and Senescence
Stanford University
2016-2025
Stanford Medicine
2020-2025
Palo Alto University
2024
Stratford University
2022-2023
Stanford Health Care
2019-2022
University of Bari Aldo Moro
2022
Surrey Memorial Hospital
2021
National Center for Advancing Translational Sciences
2020
University of North Carolina at Chapel Hill
2006-2008
The ability to maintain quiescence is critical for the long-term maintenance of a functional stem cell pool. To date, epigenetic and transcriptional characteristics quiescent cells how they change with age remain largely unknown. In this study, we explore chromatin features adult skeletal muscle cells, or satellite (SCs), which reside predominantly in state fully developed limb muscles both young aged mice. Using ChIP-seq approach obtain global profiles SCs (QSCs), show that QSCs possess...
During the SARS-CoV-2 pandemic, novel and traditional vaccine strategies have been deployed globally. We investigated whether antibodies stimulated by mRNA vaccination (BNT162b2), including third-dose boosting, differ from those generated infection or adenoviral (ChAdOx1-S Gam-COVID-Vac) inactivated viral (BBIBP-CorV) vaccines. analyzed human lymph nodes after for correlates of serological differences. Antibody breadth against variants is lower compared with all vaccines evaluated but...
Synovial sarcoma (SS) is defined by the hallmark SS18-SSX fusion oncoprotein, which renders BAF complexes aberrant in two manners: gain of SSX to SS18 subunit and concomitant loss BAF47 assembly. Here we demonstrate that globally hijacks on chromatin activate an SS transcriptional signature define using primary tumors cell lines. Specifically, retargets from enhancers broad polycomb domains oppose PRC2-mediated repression bivalent genes. Upon suppression SS18-SSX, reassembly restores...
Adult skeletal muscle stem cells, or satellite cells (SCs), regenerate functional following transplantation into injured diseased tissue. To gain insight human SC (huSC) biology, we analyzed transcriptome dynamics by RNA sequencing of prospectively isolated quiescent and activated huSCs. This analysis indicated that huSCs differentiate lose proliferative potential when maintained in high-mitogen conditions ex vivo. Further gene expression revealed p38 MAPK acts a transcriptional network...
Abstract Type 2 diabetes mellitus is a major risk factor for hepatocellular carcinoma (HCC). Changes in extracellular matrix (ECM) mechanics contribute to cancer development 1,2 , and increased stiffness known promote HCC progression cirrhotic conditions 3,4 . characterized by an accumulation of advanced glycation end-products (AGEs) the ECM; however, how this affects non-cirrhotic unclear. Here we find that, patients animal models, AGEs changes collagen architecture enhance ECM...
Abstract Tumor-associated macrophages are transcriptionally heterogeneous, but the spatial distribution and cell interactions that shape macrophage tissue roles remain poorly characterized. Here, we spatially resolve five distinct human populations in normal malignant breast colon reveal their cellular associations. This map reveals reside segregated micro-environmental niches with conserved compositions repeated across healthy diseased tissue. We show IL4I1+ phagocytose dying cells areas...
Myxoid liposarcoma is a malignant adipogenic neoplasm characterized by prominent arborizing capillaries, occasional lipoblasts, and primitive-appearing spindle cells in myxoid background. A recurrent translocation results an oncoprotein consisting of full-length DDIT3 (CHOP) fused to N-terminal segment either FUS (TLS) or, less often, EWSR1. Here, we explore the diagnostic significance expression using mouse monoclonal antibody recognizing epitope region. Studying total 300 tumors, find...
Multiplex immunofluorescence (mIF) assays multiple protein biomarkers on a single tissue section. Recently, high-plex CODEX (co-detection by indexing) systems enable simultaneous imaging of 40+ biomarkers, unlocking more detailed molecular phenotyping, leading to richer insights into cellular interactions and disease. However, data can be slower costly collect, limiting its applications, especially in clinical settings. We propose machine learning framework, 7-UP, that computationally...
Calcified chondroid mesenchymal neoplasm is a term proposed for tumors with spectrum of morphologic features, including cartilage/chondroid matrix formation, that frequently harbor FN1 gene fusions. We report series 33 cases putative calcified neoplasms, mostly referred expert consultation out concern malignancy. Patients included 17 males and 16 females, mean age 51.3 years. Anatomic locations include the hands fingers, feet toes, head neck, temporomandibular joint; 1 patient presented...
Noninfectious gastrointestinal (GI) vasculopathic disorders are rare and often overlooked in histopathologic examination or when forming differential diagnoses due to their rarity. However, involvement of the GI tract may lead serious complications, including ischemia perforation. Since awareness types vasculopathy that involve is central arriving at a correct diagnosis, we reviewed our institutional experience with order enhance diagnostic accuracy these lesions. We report clinical...
Type 2 diabetes is clinically associated with progressive necroinflammation and fibrosis in nonalcoholic steatohepatitis (NASH). Advanced glycation end-products (AGEs) accumulate during prolonged hyperglycemia, but the mechanistic pathways that lead to accelerated liver have not been well defined. In this study, we show AGEs clearance receptor AGER1 was downregulated patients NASH our models, whereas proinflammatory RAGE induced. These findings were necroinflammatory, fibrogenic, pro-oxidant...
Tumor-associated macrophages (TAMs) display heterogeneous phenotypes. Yet the exact tissue cues that shape macrophage functional diversity are incompletely understood. Here we discriminate, spatially resolve and reveal function of five distinct niches within malignant benign breast colon tissue. We found SPP1 TAMs reside in hypoxic necrotic tumor regions, a novel subset FOLR2 resident (TRMs) supports plasma cell niche. discover IL4I1 populate with high turnover where they phagocytose dying...