A5086444587- Cellular Mechanics and Interactions
- Extracellular vesicles in disease
- Microtubule and mitosis dynamics
- Cellular transport and secretion
- Immune cells in cancer
- Nanoplatforms for cancer theranostics
- Wnt/β-catenin signaling in development and cancer
- Cell Adhesion Molecules Research
- RNA Interference and Gene Delivery
- Protein Kinase Regulation and GTPase Signaling
- Mitochondrial Function and Pathology
- Ubiquitin and proteasome pathways
- MicroRNA in disease regulation
- Cancer Research and Treatments
- Glycosylation and Glycoproteins Research
- Gene Regulatory Network Analysis
- Glioma Diagnosis and Treatment
- Single-cell and spatial transcriptomics
- RNA regulation and disease
- S100 Proteins and Annexins
- Peptidase Inhibition and Analysis
- Hippo pathway signaling and YAP/TAZ
- RNA Research and Splicing
- Lipid Membrane Structure and Behavior
- Cancer-related Molecular Pathways
Children's Cancer and Blood Foundation
2021
Cornell University
2020-2021
Weill Cornell Medicine
2021
Albert Einstein College of Medicine
2014-2020
Lebanese American University
2012-2014
Yeshiva University
2014
Macrophage interactions with other cells, either locally or at distances, are imperative in both normal and pathological conditions. While soluble means of communication can transmit signals between different it does not account for all long distance macrophage interactions. Recently described tunneling nanotubes (TNTs) membranous channels that connect cells together allow transfer signals, vesicles, organelles. However, very little is known about the mechanism by which these structures...
The interaction between tumor cells and macrophages is crucial in promoting invasion metastasis. In this study, we examined a novel mechanism of intercellular communication, namely membranous actin-based tunneling nanotubes (TNTs), that occurs the promotion macrophage-dependent cell invasion. presence heterotypic TNTs induced invasive morphology, which was dependent on EGF-EGFR signaling. Furthermore, reduction protein involved TNT formation, M-Sec (TNFAIP2), inhibited elongation, blocked...
Cdc42 is critical in a myriad of cellular morphogenic processes, requiring precisely regulated activation dynamics to affect specific events. To facilitate direct observations live cells, we developed and validated new biosensor activation. The genetically encoded, single-chain design capable correctly localizing membrane compartments as well interacting with its upstream regulators including the guanine nucleotide dissociation inhibitor. We characterized this motile mouse embryonic...
Breast cancer is one of the most commonly diagnosed cancers in women around world. In general, more aggressive tumor, rapidly it grows and likely metastasizes. Members Rho subfamily small GTP-binding proteins (GTPases) play a central role breast cell motility metastasis. The switch between active GTP-bound inactive GDP-bound state regulated by guanine nucleotide exchange factors (GEFs), GTPase-activating (GAPs) guanine-nucleotide dissociation inhibitors (GDIs). We studied StarD13, recently...
We have shown previously that tyrosine phosphorylation of Wiskott-Aldrich syndrome protein (WASP) is important for diverse macrophage functions including phagocytosis, chemotaxis, podosome dynamics, and matrix degradation. However, the specific kinase mediating WASP still unclear. Here, we provide evidence Hck, which predominantly expressed in leukocytes, can phosphorylate regulates WASP-mediated functions. demonstrate response to stimulation with CX3CL1 or via Fcγ receptor ligation were...
Abstract Background Metastasis is the cause of most cancer‐related deaths. It known that breast cancer cells in proximity to macrophages become more invasive an Epidermal Growth Factor (EGF) dependent manner. Tunneling nanotubes (TNTs) are thin, F–actin containing, cellular protrusions mediate intercellular communication and have been identified many tumors. The mechanism TNT formation varies between different cell types. M‐Sec (TNFAIP2) has demonstrated be involved some types including...
Abstract Background Lung cancer is the second most commonly occurring cancer. The ability to metastasize and spread distant locations renders tumor more aggressive. Members of Rho subfamily small GTP-binding proteins (GTPases) play a central role in regulation actin cytoskeleton cell migration metastasis. In this study we investigated RhoA/Cdc42 GAP, StarD13, previously described suppressor, malignancy, invasion lung cells A549. Methods We knocked down StarD13 expression A549 tested effect...
Purpose: Extracellular vesicles (EVs) contain RNA and protein cargo reflective of the genotype phenotype releasing cell origin. Adult neural retina EV release, transfer, proteomic are focus this study. Methods: wild-type mouse retinae were cultured released diameters concentrations quantified using Nanosight. Immunogold transmission electron microscopy (TEM) was used to image ultrastructure marker localization. Quantitative real-time polymerase chain reaction (qRT-PCR) analyze retinal...
Metastasis remains the main challenge to overcome for treating ovarian cancers. In this study, we investigate potential role of Cdc42 GAP StarD13 in modulation cell motility, invasion cancer cells. depletion does not affect 2D motility More importantly, inhibits matrix degradation, invadopodia formation and through inhibition Cdc42. localize mature TKS4-labeled that possess degradation ability, while a FRET biosensor, detects activation these invadopodia. fact, localization appear mutually...
Abstract The interaction between tumor cells and macrophages is crucial in promoting invasion metastasis. These two cell types are engaged a mutual which tumor-associated produce epidermal growth factor (EGF) to activate cells. In turn, colony-stimulating factor-1 (CSF-1) that stimulates macrophages. - macrophage pairs formed response this paracrine signaling then attracted toward blood vessels under an endothelial produced HGF gradient. This leads the co-migration of both as imaged vitro...