A5035016931- Microtubule and mitosis dynamics
- Cellular Mechanics and Interactions
- Pluripotent Stem Cells Research
- RNA Research and Splicing
- Cell Image Analysis Techniques
- Medical Image Segmentation Techniques
- AI in cancer detection
- Cancer-related Molecular Pathways
- Congenital heart defects research
- Genomics and Chromatin Dynamics
- Skin and Cellular Biology Research
- CRISPR and Genetic Engineering
- Wnt/β-catenin signaling in development and cancer
- Developmental Biology and Gene Regulation
- Digital Imaging for Blood Diseases
- Reproductive Biology and Fertility
- Epigenetics and DNA Methylation
- Signaling Pathways in Disease
- Melanoma and MAPK Pathways
- Renal and related cancers
- Protein Kinase Regulation and GTPase Signaling
- Caveolin-1 and cellular processes
- Tissue Engineering and Regenerative Medicine
- Advanced Fluorescence Microscopy Techniques
- Bacillus and Francisella bacterial research
National Institutes of Health
2025
National Cancer Institute
2021-2025
Center for Cancer Research
2025
National Human Genome Research Institute
2022-2024
Université Libre de Bruxelles
2016-2022
Institut Belge pour la Sécurité Routière
2017
Lebanese American University
2013-2016
In mouse embryo gastrulation, epiblast cells delaminate at the primitive streak to form mesoderm and definitive endoderm, through an epithelial-mesenchymal transition. Mosaic expression of a membrane reporter in nascent enabled recording cell shape trajectory live imaging. Upon leaving streak, changed extended protrusions distinct size abundance depending on neighboring germ layer, as well region embryo. Embryonic trajectories were meandrous but directional, while extra-embryonic showed...
Cyclin-dependent kinases (CDKs) regulate proliferation dynamics and cell fate in response to extracellular inputs. It remains largely unknown how CDK activity fluctuates influences commitment during early mammalian development. Here, we generated a mouse model expressing translocation reporter that enabled quantification of live single cells. By examining pre- post-implantation embryos at different stages, observed progressive decrease cells from the trophectoderm (TE) prior implantation....
Breast cancer is one of the most commonly diagnosed cancers in women around world. In general, more aggressive tumor, rapidly it grows and likely metastasizes. Members Rho subfamily small GTP-binding proteins (GTPases) play a central role breast cell motility metastasis. The switch between active GTP-bound inactive GDP-bound state regulated by guanine nucleotide exchange factors (GEFs), GTPase-activating (GAPs) guanine-nucleotide dissociation inhibitors (GDIs). We studied StarD13, recently...
Malignant astrocytomas are highly invasive into adjacent and distant regions of the normal brain. Understanding targeting cancer cell invasion is an important therapeutic approach. Cell a complex process that replies on many signaling pathways including mitogen-activated protein (MAP) kinase (MAPK). In lines, use MAPK-targeted drugs proved to be potential method inhibit motility. present study, we recombinant anthrax lethal toxin (LeTx), which selectively inhibits MAPK pathway, in order...
ABSTRACT Upon exit from the totipotent 2-cell (2C) embryo stage, 2C-associated transcriptional program needs to be efficiently silenced. However, molecular mechanisms involved in this process remain mostly unknown. Here, we demonstrate that 2C-specific transcription factor DUX directly induces expression of DUXBL promote silencing. Indeed, Duxbl -knockout ESC causes increased induction 2C-transcriptional program, whereas overexpression impairs transcription. CUT&RUN analyses show gains...
The progress in imaging techniques have allowed the study of various aspect cellular mechanisms. To isolate individual cells live data, we introduce an elegant image segmentation framework that effectively extracts cell boundaries, even presence poor edge details. Our approach works two stages. First, estimate pixel interior/border/exterior class probabilities using random ferns. Then, use energy minimization to compute boundaries whose localization is compliant with probabilities. We...
ABSTRACT The progress in imaging techniques have allowed the study of various aspect cellular mechanisms. To isolate individual cells live data, we introduce an elegant image segmentation framework that effectively extracts cell boundaries, even presence poor edge details. Our approach works two stages. First, estimate pixel interior/border/exterior class probabilities using random ferns. Then, use energy minimization to compute boundaries whose localization is compliant with probabilities....
Cyclin-dependent kinases (CDK) are key regulatory enzymes that regulate proliferation dynamics and cell fate in response to extracellular inputs. It remains largely unknown how CDK activity fluctuates influences commitment
SUMMARY After implantation, the mouse embryo undergoes gastrulation and forms mesoderm endoderm. Mesoderm participates in embryonic extra-embryonic tissues including amnion, yolk sac, chorion allantois, umbilical cord precursor. Extra-embryonic is rich intermediate filaments. Two-photon live imaging of keratin 8-eYFP knock-in embryos allowed recording nucleation elongation filaments, which formed apical cables coordinated across multiple cells blood islands. Embryos lacking all keratins...
We demonstrate that the localized introduction of cell markers by electroporation can be efficiently and safely achieved with reusable glass chips complying industrial micromachining techniques. Consequently, any biologist now rely on contract manufacturing to easily implement this transfection approach at moderate cost.
The progress in imaging techniques have allowed the study of various aspect cellular mechanisms. To isolate individual cells live data, we introduce an elegant image segmentation framework that effectively extracts cell boundaries, even presence poor edge details. Our approach works two stages. First, estimate pixel interior/border/exterior class probabilities using random ferns. Then, use energy minimization to compute boundaries whose localization is compliant with probabilities. We...
ABSTRACT In the gastrulating mouse embryo, epiblast cells delaminate at primitive streak to form mesoderm and definitive endoderm, through an epithelial-mesenchymal transition. Mosaic expression of a membrane reporter in nascent enabled recording cell shape trajectory live imaging. Upon leaving streak, changed extended protrusions distinct size abundance depending on neighboring germ layer, as well region embryo. Embryonic trajectories were meandrous but directional, while extra-embryonic...