A5053506367- Pluripotent Stem Cells Research
- Reproductive Biology and Fertility
- Cellular Mechanics and Interactions
- Photosynthetic Processes and Mechanisms
- Microtubule and mitosis dynamics
- Cancer Cells and Metastasis
- Developmental Biology and Gene Regulation
- Skin and Cellular Biology Research
- RNA Research and Splicing
- Congenital heart defects research
- Renal and related cancers
- Tissue Engineering and Regenerative Medicine
- Mesenchymal stem cell research
- Plant Molecular Biology Research
- 3D Printing in Biomedical Research
- Photochromic and Fluorescence Chemistry
- Photoreceptor and optogenetics research
- Wnt/β-catenin signaling in development and cancer
- Genetics, Aging, and Longevity in Model Organisms
- Protease and Inhibitor Mechanisms
- Cell Adhesion Molecules Research
Université Libre de Bruxelles
2018-2022
Institut Belge pour la Sécurité Routière
2017
Centre National de la Recherche Scientifique
2015
École Normale Supérieure de Lyon
2015
Université Claude Bernard Lyon 1
2015
In mouse embryo gastrulation, epiblast cells delaminate at the primitive streak to form mesoderm and definitive endoderm, through an epithelial-mesenchymal transition. Mosaic expression of a membrane reporter in nascent enabled recording cell shape trajectory live imaging. Upon leaving streak, changed extended protrusions distinct size abundance depending on neighboring germ layer, as well region embryo. Embryonic trajectories were meandrous but directional, while extra-embryonic showed...
During mitosis, chromosomes are connected to a microtubule-based spindle. Current models propose that displacement of the spindle poles and/or activity kinetochore microtubules generate mechanical forces segregate sister chromatids. Using laser destruction centrosomes during Caenorhabditis elegans we show neither these mechanisms is necessary achieve proper chromatid segregation. Our results strongly suggest an outward force generated by midzone, independently centrosomes, sufficient in...
The mechanical properties of the different germ layers early mammalian embryo are likely to be critical for morphogenesis. Cytoskeleton components (actin and myosin, microtubules, intermediate filaments) major determinants epithelial plasticity resilience stress. Here, we take advantage a mouse reporter Keratin 8 record pattern keratin filaments network in first epithelia developing embryo. At blastocyst stage, is strongly expressed trophectoderm, undetectable inner cell mass its...
ABSTRACT The epiblast, a pseudostratified epithelium, is the precursor for three main germ layers required body shape and organogenesis: ectoderm, mesoderm, endoderm. At gastrulation, subpopulation of epiblast cells constitutes transient posteriorly located structure called primitive streak, where that undergo epithelial-mesenchymal transition make up mesoderm endoderm lineages. In order to observe behavior individual cells, were labeled ubiquitously or in mosaic fashion using fluorescent...
SUMMARY After implantation, the mouse embryo undergoes gastrulation and forms mesoderm endoderm. Mesoderm participates in embryonic extra-embryonic tissues including amnion, yolk sac, chorion allantois, umbilical cord precursor. Extra-embryonic is rich intermediate filaments. Two-photon live imaging of keratin 8-eYFP knock-in embryos allowed recording nucleation elongation filaments, which formed apical cables coordinated across multiple cells blood islands. Embryos lacking all keratins...
ABSTRACT In the gastrulating mouse embryo, epiblast cells delaminate at primitive streak to form mesoderm and definitive endoderm, through an epithelial-mesenchymal transition. Mosaic expression of a membrane reporter in nascent enabled recording cell shape trajectory live imaging. Upon leaving streak, changed extended protrusions distinct size abundance depending on neighboring germ layer, as well region embryo. Embryonic trajectories were meandrous but directional, while extra-embryonic...