A5059781163- Virus-based gene therapy research
- Cytokine Signaling Pathways and Interactions
- CAR-T cell therapy research
- Toxin Mechanisms and Immunotoxins
- Endoplasmic Reticulum Stress and Disease
- Viral Infectious Diseases and Gene Expression in Insects
- Cancer-related Molecular Pathways
- Cancer Research and Treatments
- Pharmacological Receptor Mechanisms and Effects
- Tryptophan and brain disorders
- RNA Research and Splicing
- Pharmacological Effects of Natural Compounds
- Retinal Development and Disorders
- RNA and protein synthesis mechanisms
- Cell Adhesion Molecules Research
- Cancer, Hypoxia, and Metabolism
- RNA modifications and cancer
- Cancer Mechanisms and Therapy
- Immunotherapy and Immune Responses
- Protein Kinase Regulation and GTPase Signaling
- Ubiquitin and proteasome pathways
- Cell death mechanisms and regulation
- 3D Printing in Biomedical Research
- RNA regulation and disease
- Circular RNAs in diseases
The Graduate Center, CUNY
2015-2025
City University of New York
2013-2025
Lehman College
2014-2025
Columbia University
2002-2010
Virginia Commonwealth University
2009
Columbia University Irving Medical Center
2004-2008
Fundación Instituto Leloir
1997-2005
Virginia Commonwealth University Medical Center
2002
University of Liverpool
2000
Subtraction hybridization identified melanoma differentiation-associated gene-7 (mda-7) as a gene induced during terminal differentiation in human cells. On the basis of structure, chromosomal localization and cytokine-like properties, mda-7 is classified IL-24. Administration mda-7/IL-24 by means replication-incompetent adenovirus (Ad.mda-7) induces apoptosis selectively diverse cancer cells without inducing harmful effects normal fibroblast or epithelial The present studies investigated...
A noteworthy aspect of melanoma differentiation-associated gene-7/interleukin-24 (mda-7/IL-24) as a cancer therapeutic is its ability to selectively kill cells without harming normal cells. Intracellular MDA-7/IL-24 protein, generated from an adenovirus expressing mda-7/IL-24 (Ad.mda-7), induces cancer-specific apoptosis by inducing endoplasmic reticulum (ER) stress response. Secreted infected with Ad.mda-7, growth inhibition and in surrounding noninfected but not cells, thus exerting...
Subtraction hybridization applied to a 'differentiation therapy' model of cancer employing human melanoma cells resulted in the cloning differentiation associated gene-7/interleukin-24 (mda-7/IL-24). Initial studies confirm an inverse correlation between mda-7 expression and development progression. Forced by means plasmid or via replication incompetent adenovirus (Ad.mda-7) promotes growth suppression induces apoptosis broad array cancers. In contrast, does not induce suppressive toxic...
Breast cancer treatment has advanced significantly, particularly for estrogen receptor-positive (ER+) tumors. Tamoxifen, an antagonist, is widely used; however, approximately 40% of patients develop resistance. Recent studies indicate that microRNAs, especially miR-155, play a critical role in this Our analysis MCF-7 tamoxifen-sensitive (TAM-S) and tamoxifen-resistant (TAM-R) cells revealed miR-155 significantly upregulated TAM-R cells. Overexpression TAM-S increased resistance to tamoxifen....
Interleukin 24 (IL-24) is a tumor-suppressing protein currently in clinical trials. We previously demonstrated that IL-24 leads to apoptosis cancer cells through kinase A (PKA) activation human breast cells. To better understand the mechanism by which induces apoptosis, we analyzed role of glycogen synthase kinase-3 beta (GSK3β), highly conserved serine/threonine and downstream target PKA. Our studies show for first time GSK3β inhibited following treatment prostate showed inhibition mediated...
Abstract Experimental evidence documents that the MDA‐7 / IL‐24 protein (an IL‐10 family cytokine) binds to IL‐20 and IL‐22 receptor complexes resulting in activation of JAK/STAT signaling pathways. Recent published reports utilizing human blood derived primary lymphocytes have provided additional confirmatory relating cytokine properties this molecule. A notable attribute mda‐7 is its cancer cell‐specific apoptosis inducing capacity, which currently remains incompletely understood....
Abstract Melanoma differentiation associated gene-7/interleukin-24 (Mda-7/IL-24), a novel member of the IL-10 family cytokines, uniquely displays cancer-specific apoptosis-inducing activity. Positive results in ongoing phase I/II clinical trials have strengthened possibility its utilization as cancer gene therapeutic. Previous studies document that signaling events leading to Ad.mda-7-induced transformed cell apoptosis are tyrosine kinase-independent. These suggest mda-7/IL-24 cell-specific...
Abstract Melanoma differentiation-associated gene-7/interleukin-24 (mda-7/IL-24) is a novel cytokine displaying selective apoptosis-inducing activity in transformed cells without harming normal cells. The present studies focused on defining the mechanism(s) by which GST-MDA-7 fusion protein inhibits cell survival of primary human glioma vitro. killed with diverse genetic characteristics that correlated inactivation ERK1/2 and activation JNK1-3. Activation JNK1-3 was dependent kinase R–like...
Abstract Cell migration and invasion are critical events during the progression to metastasis. Without motile function, cancer cells unable leave primary tumor site, invade through basement membrane, form secondary tumors. Expression of epithelial-specific ETS factor prostate-derived (PDEF) is reduced in human invasive breast tissue lost cell lines. Gain-of-function studies that examine different aspects show constitutive or inducible PDEF reexpression inhibits multiple lines,...
Melanoma differentiation associated gene-7/interleukin-24 (mda-7/IL-24) uniquely displays broad cancer-specific apoptosis-inducing activity through induction of endoplasmic reticulum (ER) stress. We hypothesize that ceramide, a promoter apoptosis, might contribute to mda-7/IL-24 apoptosis. Ad.mda-7-infected tumor cells, but not normal showed increased ceramide accumulation. Infection with Ad.mda-7 induced marked increase in various ceramides (C16, C24, C24:1) selectively prostate cancer...
One hallmark of neoplasia is abnormal differentiation. Induction differentiation, by chemical or biological methods, provides a possible therapeutic intervention. "Differentiation therapy" well documented in several model systems. These include melanoma, which treatment with interferon-beta and the protein kinase C activator mezerein induces irreversible growth arrest terminal differentiation culminating programmed cell death. Subtraction hybridization between terminally differentiated...