A5030420068- Advanced Glycation End Products research
- Prostate Cancer Treatment and Research
- MicroRNA in disease regulation
- Tannin, Tannase and Anticancer Activities
- Nutritional Studies and Diet
- Alcohol Consumption and Health Effects
- DNA Repair Mechanisms
- Cancer, Hypoxia, and Metabolism
- Circular RNAs in diseases
- Nutrition, Genetics, and Disease
- Fatty Acid Research and Health
- Ubiquitin and proteasome pathways
- Cancer Cells and Metastasis
- Cancer, Lipids, and Metabolism
- Ferroptosis and cancer prognosis
- Natural Antidiabetic Agents Studies
- Cancer Risks and Factors
- Genetic factors in colorectal cancer
- Phytoestrogen effects and research
- RNA Research and Splicing
- Soil Carbon and Nitrogen Dynamics
- Monoclonal and Polyclonal Antibodies Research
- Cancer survivorship and care
- Estrogen and related hormone effects
- RNA modifications and cancer
Frederick National Laboratory for Cancer Research
2020-2024
Virginia Commonwealth University
2023-2024
Leidos Biomedical Research Inc. (United States)
2024
Leidos (United States)
2020-2024
Medical University of South Carolina
2014-2023
MUSC Hollings Cancer Center
2009-2023
VCU Massey Comprehensive Cancer Center
2023
Monash University
2023
Virginia Cancer Institute
2023
St Vincent's Hospital
2021
Abstract The glycine to cysteine mutation on codon 12 of KRAS (KRASG12C) is found in ~15% non-small cell lung cancers and a low percentage colorectal pancreatic adenocarcinomas. This activating pushes the balance cellular towards its active, GTP-bound (ON) state that signals downstream drives proliferation. Recently approved inhibitors KRASG12C bind sequester oncogenic protein inactive GDP-bound (OFF) state, have demonstrated clinical efficacy; however, median duration response has been...
Cytotoxicity of methylating agents is caused mostly by methylation the O 6 position guanine in DNA to form –methylguanine ( –meG). –meG can direct misincorporation thymine during replication, generating –meG:T mismatches. Recognition these mispairs mismatch repair (MMR) system leads cell cycle arrest and apoptosis. MMR also modulates sensitivity other antitumor drugs. The base excision (BER) enzyme MED1 (also known as MBD4) interacts with protein MLH1. was found exhibit glycosylase activity...
Abstract Introduction MicroRNAs are small non-coding RNAs that involved in the post-transcriptional negative regulation of mRNAs. MicroRNA 510 (miR-510) was initially shown to have a potential oncogenic role breast cancer by observation its elevated levels human tumor samples when compared matched non-tumor samples. Few targets been identified for miR-510. However, as microRNAs function through their direct targets, identification those is critical understanding functional cancer. Methods...
Lifestyle factors associated with personal behavior can alter tumor-associated biological pathways and thereby increase cancer risk, growth, disease recurrence. Advanced glycation end products (AGEs) are reactive metabolites produced endogenously as a by-product of normal metabolism. A Western lifestyle also promotes AGE accumulation in the body which is phenotypes through modification genome, protein crosslinking/dysfunction, aberrant cell signaling. Given links between lifestyle, AGEs,...
Abstract Cell migration and invasion are critical events during the progression to metastasis. Without motile function, cancer cells unable leave primary tumor site, invade through basement membrane, form secondary tumors. Expression of epithelial-specific ETS factor prostate-derived (PDEF) is reduced in human invasive breast tissue lost cell lines. Gain-of-function studies that examine different aspects show constitutive or inducible PDEF reexpression inhibits multiple lines,...
Abstract Prostate-derived Ets factor (PDEF) is an ETS transcription expressed in normal tissues with high epithelial cell content and noninvasive breast cancer cells. A putative tumor suppressor PDEF protein expression often lost during progression to a more invasive phenotype. Interestingly, mRNA has been found be retained or even overexpressed the absence of protein; however, mechanisms for this remain elucidated. This study identifies two microRNAs (miRNA) that directly act on repress...
Abstract BACKGROUND Ets is a large family of transcriptional regulators with functions in most biological processes. While the gene, prostate‐derived epithelial factor (PDEF), expressed tissues, PDEF protein expression has been found to be reduced or lost during cancer progression. The goal this study was examine mechanism for and biologic impact altered prostate cancer. METHODS specimens examined by immunohistochemistry. RNA cell lines were measured q‐PCR Western blot, respectively....
Abstract Socioeconomic and environmental influences are established factors promoting cancer disparity, but the contribution of biologic is not clear. We report a mechanistic link between carbohydrate-derived metabolites that may provide consequence disparity. Glycation nonenzymatic glycosylation carbohydrates to macromolecules, which produces reactive called advanced glycation end products (AGE). A sedentary lifestyle poor diet all promote disease AGE accumulation pool in our bodies also...
Abstract Advanced glycation end-products (AGEs) are implicated in the pathogenesis of several chronic diseases including cancer. AGEs produced endogenously but can also be consumed from foods. AGE formation food is accelerated during cooking at high temperatures. Certain fat or highly processed foods have values. The objective study was to assign and quantify Nε-carboxymethyl-lysine (CML)-AGE content investigate association between dietary intake breast cancer risk Prostate, Lung, Colorectal...
Pulmonary rehabilitation (PR) is an accepted therapy for patients with chronic obstructive pulmonary disease (COPD), improving both exercise capacity and quality of life (QOL). Generic measures QOL have been criticized as being insensitive to detecting the improvement in after PR contrast disease-specific instruments. The authors looked at Medical Outcomes Survey Short Form 36-item questionnaire (SF-36), a generic measure, detect changes COPD completion PR.Patients who participated program...
Targeting the tumor microenvironment is critical toward improving effectiveness of cancer therapeutics. Cancer-associated fibroblasts (CAFs) are one most abundant cell types microenvironment, playing an important role in progression. Multiple origins for CAFs have been proposed including resident fibroblasts, adipocytes, and bone marrow. Our laboratory previously identified a novel hematopoietic stem (HSC) origin CAFs; however, functional roles HSC-derived (HSC-CAFs) progression not yet...
Background Advanced glycation end products (AGEs) are reactive metabolites produced as a by‐product of sugar metabolism and consumed through the diet in high‐fat highly processed foods. They associated with chronic inflammatory diseases, evidence suggests that they play role carcinogenesis. The authors evaluated association dietary AGE intake risk postmenopausal invasive breast cancer. Methods This was prospective cohort study 183,548 women National Institutes Health‐AARP Diet Health Study....
Development of new targeted inhibitors for oncogenic KRAS mutants may benefit from insight into how a given mutation influences the accessibility protein residues and compounds interact with mutant or wild-type proteins. Targeted proteomic analysis, key validation step in inhibitor development process, typically involves both intact mass- peptide-based methods to confirm compound localization quantify binding. However, these not always provide clear picture binding affinity KRAS, specific is...
Abstract PI3Kα is the most mutated kinase and second oncogene in human cancer. Activation of PI3Ka can be achieved by receptor tyrosine kinases such as insulin insulin-like growth factor 1 and/or directly interacting with RAS family members. Previous elegant preclinical studies have established that RAS-driven activation important tumor cells but may not involved cell types controlling glucose metabolism. Alpelisib, a small molecule inhibitor activity PI3Ka, has been approved for treatment...
Social, environmental, and biological risk factors influence exposures to newly termed 'biosocial determinants of health'. As molecular that lie at the intersection between lived experiences individual biology, biosocial may inform on enduring complexity cancer disparity across transdisciplinary studies.
Abstract The base excision repair protein MED1 (also known as MBD4), an interactor with the mismatch MLH1, has a central role in maintenance of genomic stability dual functions DNA damage response and repair. acts thymine uracil N-glycosylase on T:G U:G mismatches that occur at cytosine-phosphate-guanine (CpG) methylation sites due to spontaneous deamination 5-methylcytosine cytosine, respectively. To elucidate mechanisms underlie sequence discrimination by MED1, we did single-turnover...
Prostate derived ETS factor (PDEF) is an (epithelial-specific E26 transforming sequence) family member that has been identified as a potential tumor suppressor. In multiple invasive breast cancer cells, PDEF expression inhibits cell migration by preventing the acquisition of directional morphological polarity conferred changes in cytoskeleton organization. this study, microarray analysis was used to identify >200 human genes displayed common differential pattern three lines after exogenous...
The Ets family of transcription factors control a myriad cellular processes and contribute to the underlying genetic loss homeostasis resulting in cancer. PDEF (prostate-derived factor) has been under investigation for its role tumor development progression. However, cancer controversial. Some reports link promoter, others show tumor-suppressing functions various systems different conditions. So far, there no conclusive evidence from vivo experiments prove PDEF. We have used both vitro...