A5077715945- Advanced Fluorescence Microscopy Techniques
- Cell Image Analysis Techniques
- CRISPR and Genetic Engineering
- Monoclonal and Polyclonal Antibodies Research
- Single-cell and spatial transcriptomics
- Biochemical and Structural Characterization
- Ubiquitin and proteasome pathways
- Protein Kinase Regulation and GTPase Signaling
- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- Electrospun Nanofibers in Biomedical Applications
- Photonic and Optical Devices
- Meat and Animal Product Quality
- Surgical Sutures and Adhesives
- Transgenic Plants and Applications
- Near-Field Optical Microscopy
- Digital Imaging in Medicine
- Organ and Tissue Transplantation Research
- PI3K/AKT/mTOR signaling in cancer
- Protein Degradation and Inhibitors
- Prostate Cancer Treatment and Research
- Optical Imaging and Spectroscopy Techniques
- Advanced Photocatalysis Techniques
- Advancements in Photolithography Techniques
- Cellular and Composite Structures
Fanjingshan National Nature Reserve
2025
Jiangsu University
2020-2024
Beijing Academy of Quantum Information Sciences
2024
Xinyang Normal University
2024
Io Therapeutics (United States)
2024
City College of San Francisco
2023
East China University of Science and Technology
2023
University of California, San Francisco
2017-2022
Anhui University of Finance and Economics
2022
University of California, Berkeley
2017-2021
Self-complementing split fluorescent proteins (FPs) have been widely used for protein labeling, visualization of subcellular localization, and detection cell-cell contact. To expand this toolset, we developed a screening strategy the direct engineering self-complementing FPs. Via strategy, generated yellow-green split-mNeonGreen21-10/11 that improves ratio complemented signal to background FP1-10-expressing cells compared commonly GFP1-10/11; as well 10-fold brighter red-colored...
Receptor tyrosine kinase (RTK)-mediated activation of downstream effector pathways such as the RAS GTPase/MAP (MAPK) signaling cascade is thought to occur exclusively from lipid membrane compartments in mammalian cells. Here, we uncover a membraneless, protein granule-based subcellular structure that can organize RTK/RAS/MAPK cancer. Chimeric (fusion) oncoproteins involving certain RTKs including ALK and RET undergo de novo higher-order assembly into membraneless cytoplasmic granules...
Significance Optimal cell delivery strategies are in urgent need to enhance the specificity, efficacy, and reproducibility of therapy leading minimized dosage side effects. Here, we addressed this unmet by developing injectable 3D microscale cellular niches (microniches) based on biocompatible biodegradable gelatin microcryogels. Dramatic improvement retention, survival, therapeutic effects enabled primed microniches was demonstrated treatment critical limb ischemia (CLI) mouse models...
Expansion microscopy (ExM) increases the effective resolving power of any microscope by expanding sample with swellable hydrogel. Since its invention, ExM has been successfully applied to a wide range cell, tissue, and animal samples. Still, fluorescence signal loss during polymerization digestion limits molecular-scale imaging using ExM. Here, we report development label-retention (LR-ExM) set trifunctional anchors that not only prevent but also enable high-efficiency labeling SNAP CLIP...
Abstract Self-associating split fluorescent proteins (FPs) are FPs whose two fragments spontaneously associate to form a functional FP. They have been widely used for labeling proteins, scaffolding protein assembly and detecting cell-cell contacts. Recently developments expanded the palette of self-associating beyond original GFP 1-10/11 . However, these new ones suffered from suboptimal fluorescence signal after complementation. Here, by investigating complementation process, we...
Background: The phylogenetic resolution within the Gloydius halys-intermedius Complex remains debatable due to following reasons: loci selection in previous studies varied between authors; limited dataset (1−5 mitochondrial or nuclear gene fragments); lack of sampling density; and nodal supports at specific nodes remain weak, specifically cognatus, G. halys, stejnegeri. Objectives: To revise taxonomic relationships Complex, we reconstructed molecular phylogeny performed species delimitation...
Thermal asymmetric interlaced (TAIL)-PCR-based and whole-genome sequencing-based T-DNA localization approaches have been developed for the recovery of integration sites (TISs). Nevertheless, a low-cost high-throughput technique detection TISs, which would facilitate identification genetically engineered plants, is in high demand rapid crop breeding plant synthetic biology. Here, we present Tn5 transposase-based Integration Site Localization (TTLOC), Tn5-based approach TIS localization. TTLOC...
Fluorescent sensors targeted to endogenous proteins measure native signaling dynamics within compartments.
Mutant-specific inhibitors of KRASG12C, such as AMG510 (sotorasib) and MRTX849 (adagrasib), offer the unprecedented opportunity to inhibit KRAS, most frequently mutated heretofore undruggable oncoprotein. While clinical data are still limited, on-target mutations in KRASG12C at position 12 other sites emerging major drivers relapse. We identified additional that impact inhibitor sensitivity through a saturation mutagenesis screen NCI-H358 non–small-cell lung cancer (NSCLC) cell line. also...
Microtissues on the scale of several hundred microns are a promising cell culture configuration resembling functional tissue units <italic>in vivo</italic>.
Labeling proteins with high specificity and efficiency is a fundamental prerequisite for microscopic visualization of subcellular protein structures interactions. Although the comparatively small size epitope tags makes them less perturbative to fusion proteins, they require use large antibodies that often limit probe accessibility effective resolution. Here we covalent SpyTag-SpyCatcher system as an epitope-like tag fluorescent labeling intracellular in fixed cells both conventional...
Abstract Expansion microscopy (ExM) increases the effective resolving power of any microscope by expanding sample with swellable hydrogel. Since its invention, ExM has been successfully applied to a wide range cell, tissue and animal samples. Still, fluorescence signal loss during polymerization digestion limits molecular-scale imaging using ExM. Here we report development label-retention (LR-ExM) set trifunctional anchors that not only prevent but also enable high-efficiency labeling SNAP...
Abstract PI3Kα is the most mutated kinase and second oncogene in human cancer. Activation of PI3Ka can be achieved by receptor tyrosine kinases such as insulin insulin-like growth factor 1 and/or directly interacting with RAS family members. Previous elegant preclinical studies have established that RAS-driven activation important tumor cells but may not involved cell types controlling glucose metabolism. Alpelisib, a small molecule inhibitor activity PI3Ka, has been approved for treatment...
A new approach for fabrication of a long-term and recoverable antimicrobial nanostructure/textile hybrid without increasing the resistance is demonstrated. Using in situ synthesized Ag nanoparticles (NPs) anchored on ZnO nanowires (NWs) grown textiles by 'dip-in light-irradiation' green chemical method, we obtained ZnONW@AgNP nanocomposites with small-size uniform NPs, which have shown superior performance antibacterial applications. These Ag/ZnO/textile composites can be used wound...
Abstract This work presents a novel method for eggshell crack timely detection of hen and duck eggs based on the acoustic resonance analysis. The developed experimental device consisting an excitation device, acquisition module signals, personal computer was designed to generate acquire response signals by impaction eggshell. parameter Pearson's correlation coefficients (PCCs) calculated compare similarity between intact with various sampling points. 200 points signal were selected as...
<h3>Importance</h3> Increasing bystander cardiopulmonary resuscitation (CPR) among racial/ethnic minority groups and culturally underserved populations is a key strategy in improving health care disparities out-of-hospital cardiac arrest. <h3>Objective</h3> To ascertain whether implementation of the Los Angeles Tiered Dispatch System (LA-TDS) was associated with improved performance telecommunicator-assisted CPR (T-CPR) 9-1-1 callers limited English proficiency City Angeles. <h3>Design,...