A5067389624- Animal Virus Infections Studies
- Computational Physics and Python Applications
- Virus-based gene therapy research
- Viral gastroenteritis research and epidemiology
- Neurogenesis and neuroplasticity mechanisms
- Viral Infections and Immunology Research
- Neutrino Physics Research
- Particle physics theoretical and experimental studies
- Tracheal and airway disorders
- Vector-Borne Animal Diseases
- Pluripotent Stem Cells Research
- Dark Matter and Cosmic Phenomena
- Nasal Surgery and Airway Studies
- Animal Disease Management and Epidemiology
- Graphene and Nanomaterials Applications
- Planarian Biology and Electrostimulation
- Nerve injury and regeneration
- Signaling Pathways in Disease
- Nanowire Synthesis and Applications
- Liver Disease and Transplantation
- Cancer-related gene regulation
- Viral-associated cancers and disorders
- Inflammasome and immune disorders
- Hearing, Cochlea, Tinnitus, Genetics
- RNA modifications and cancer
Harbin Veterinary Research Institute
2019-2024
Chinese Academy of Agricultural Sciences
2019-2024
Klinikum Bielefeld
2023
Bielefeld University
2023
Harbin Medical University
2023
Second Affiliated Hospital of Harbin Medical University
2023
Peking University International Hospital
2014-2022
University of California, Santa Barbara
2022
Peking University
2014-2022
Institute of Special Animal and Plant Sciences
2019
Pseudorabies virus (PRV), which is extremely infectious and can infect numerous mammals, has a risk of spillover into humans. Virus-host interactions determine viral entry spreading. Here, we showed that neuropilin-1 (NRP1) significantly potentiates PRV infection. Mechanistically, NRP1 promoted attachment entry, enhanced cell-to-cell fusion mediated by glycoprotein B (gB), gD, gH, gL. Furthermore, through in vitro coimmunoprecipitation (Co-IP) bimolecular fluorescence complementation (BiFC)...
New concepts for a more effective anti-cancer therapy are urgently needed. Experimental flaws represent major counter player of this development and lead to inaccurate unreproducible data as well unsuccessful translation research approaches into clinics. In previous study we have created epithelial cell cultures from head neck squamous carcinoma (HNSCC) tissue.We characterize primary populations isolated human papillomavirus positive HNSCC tissue their marker expression by RT-qPCR, flow...
A new approach for fabrication of a long-term and recoverable antimicrobial nanostructure/textile hybrid without increasing the resistance is demonstrated. Using in situ synthesized Ag nanoparticles (NPs) anchored on ZnO nanowires (NWs) grown textiles by 'dip-in light-irradiation' green chemical method, we obtained ZnONW@AgNP nanocomposites with small-size uniform NPs, which have shown superior performance antibacterial applications. These Ag/ZnO/textile composites can be used wound...
Abstract PCV3 capsid protein (Cap) is an important antigen for diagnosis and vaccine development. To achieve high-level expression of recombinant Cap in Escherichia coli ( E. ), the gene wild-type entire (wt-eCap) was amplified from clinical samples, three optimized (opti-eCap) one deleted nuclear location signal (NLS) (opti-dCap) fragments encoding same amino acid sequence with wt-eCap were synthesized based on codon bias . Those inserted into pET30a vector. One strain highest expressed...
Stem cell therapy may provide a therapeutic method for the replacement and regeneration of damaged neurons central nervous system. However, neural stem cells (NSCs) precursor (NPCs) are especially vulnerable after transplantation due to lack sufficient growth factors at transplant site. Electrical stimulation (ES) has recently been found participate in regulation proliferation, growth, differentiation, migration, but its underlying anti-apoptotic effects remain unclear. This study...
Polypyrrole (PPy), as an electrical conductive polymer, has been widely investigated in biomedical fields. In this study, PPy membrane at nanoscale was electrically deposited on indium-tin oxide glass slide with sodium p-toluenesulfonate supporting electrolyte. Electropolymerization of performed under a constant 800 mV voltage for 10 seconds. Chemical compositions and morphology were characterized by Fourier transform infrared spectroscopy (FTIR) scanning electron microscopy (SEM). The...
Little is known about the damage to important peripheral immune organ spleen caused by Streptococcus suis infection. In this study, we found that S. induced splenomegaly and lymphocyte disruption in spleens of mice. To explore mechanism splenic lesions suis, conducted further studies. The results showed apoptosis B cells, which related cleavage caspase-3 caspase-8, but not release apoptosis-inducing factor (AIF). Thus, through caspase-dependent AIF-independent pathways. Inflammation infected...
Transplantation of stem cells is a potential clinical therapy for repair central nervous system injury. However, transplanted are especially difficult to arrive at the targeted site because poor survival rate and low efficiency. Recently, gold nanoparticles (NPs) Iron oxide NPs, as novel nanoparticles, have been used auxiliary strategy investigate diseases. The present study demonstrates effect Gold/Fe 3 O 4 NPs on biocompatibility differentiated properties rat olfactory bulb cells. Cell...
Selenium materials are widely used in photoelectrical devices, owing to their unique semiconductive properties. Single‐crystal selenium nanobelts with large specific surface area, fine photoconductivity, and biocompatibility provide potential applications biomedical nanodevices, such as implantable artificial retina rapid photon detector/stimulator for optogenetics. Here, we present a nanobelt based nanodevice, which is fabricated single Se nanobelt. This device shows photo response,...
The human NOB1 gene is located at chromosome 16q22.1, encoding a protein consisting of one zinc ribbon domain. Here we aimed to efficiently generate monoclonal antibody against protein. We synthesized the peptide APVEHVVADAGAFLRH based on published cDNA sequences. was chemically linked with carrier keyhole limpet hemocyanin and then injected into Balb/c mice. Hybridomas were screened by indirect enzyme-linked immunosorbent assay (ELISA) using either purified 6xHis-NOB1 fusion or peptide. One...
The clinical outcome of porcine circovirus 3 (PCV3) infection is still controversial. Herein, a novel PCV3 isolate (PCV3-China/DB-1/2017) with the molecular characterization 24A and 27K in Cap protein was used to inoculate three-week-old cesarean-derived, colostrum-deprived piglets. nine DB-1 inoculated piglets exhibited no obvious symptoms or macroscopic lesions. displayed broad histotropism, including heart, liver, spleen, lung, kidney, brain, lymph nodes, tonsil, lungs nodes contained...
Nectin cell adhesion molecule 1 (NECTIN1) is a that belongs to the immunoglobulin superfamily. It has been considered most ubiquitous receptor for herpesviruses. However, in context of flavivirus infection, its role was previously unknown. In this study, we described an arrayed siRNA screen mainly targeting Ig-like proteins showed NECTIN1-restricted bovine viral diarrhea virus (BVDV) infection. We demonstrated depletion NECTIN1 could significantly enhance infection both biotypes and multiple...
Background: Lung adenocarcinoma (LUAD) is the most frequently occurring lung cancer worldwide, with increasing death rates. It belongs to non-small cell (NSCLC) type and has a strong association previous smoking history. Growing evidence demonstrated significance of adenosine-to-inosine RNA editing (ATIRE) dysregulation in cancer. The aim present study was evaluate ATIRE events that might be clinically useful or tumorigenic. Methods: To explore survival-related LUAD, its profiles, gene...
To elucidate the prevalence, molecular evolution, and coinfection of PCV2 PCV3 in China, we collected samples from six large-scale swine herds located Heilongjiang, Henan, Hebei, Hubei provinces Xinjiang Uygur autonomous region 2020 to 2021. Overall, 1,201 were analyzed sequenced by PCR identify dominant genotypes PCV3, genetic variations capsid protein genes evaluated. The major epidemic different across regions. infection rate was 72.1% 26.3%, respectively, 18.6%. PCV2d genotype all herds....
Porcine circovirus 3 (PCV3) is an emerging virus first discovered in the United States 2015, and since then, PCV3 has been found many regions of world, including America, Asia, Europe. Although several investigations have carried out, there a lack knowledge regarding pathogenicity PCV3, mostly due to limited number isolates that are readily available. In this study, PCV3-DB-1 was isolated PK-15 cells characterized vitro. Electron microscopy revealed presence PCV-like particles, situ...
Objective To explore the effect of biphasic electrical stimulation (BES) on proliferation and differentiation rats olfactory bulb neural precursor cells (OB NPCs). Methods OB NPCs one day old neonatal were isolated, cultured, passaged identified. They randomly divided into a control group an (ES) group. The OB in ES exposed to BES with 50 mV/mm or 100 electric field for 24 hours pulse width 8 ms, while was not given stimulation. immunofluorescent technique 3(4, 5...
Abstract Background: The rabies virus (RV) vector LBNSE expressing foreign antigens has shown considerable promise as vaccines against viral and bacteria diseases, which is effective safe. We produced a new RV-based vaccine vehicle Brucella melitensis Omp31/sodC or AHCY gene by reverse genetics technology. aim of this study was to investigate the cellular humoral immunity recombinant viruses. Results: rLBNSE-SOD rLBNSE-AHC retained growth properties similar those rLBNSE both in BSR mNA cell...