A5102877807- Hearing, Cochlea, Tinnitus, Genetics
- Traumatic Brain Injury and Neurovascular Disturbances
- Traumatic Brain Injury Research
- Ear Surgery and Otitis Media
- Neurogenesis and neuroplasticity mechanisms
- Vestibular and auditory disorders
- Cell death mechanisms and regulation
- Cardiac Arrest and Resuscitation
- Hearing Loss and Rehabilitation
- Cancer-related molecular mechanisms research
- Neuroscience and Neuropharmacology Research
- Head and Neck Surgical Oncology
- Mitochondrial Function and Pathology
- Nerve injury and regeneration
- Ear and Head Tumors
- Neuroinflammation and Neurodegeneration Mechanisms
- S100 Proteins and Annexins
- RNA Interference and Gene Delivery
- Ion channel regulation and function
- Sinusitis and nasal conditions
- Tracheal and airway disorders
- Electrospun Nanofibers in Biomedical Applications
- Acute Ischemic Stroke Management
- Facial Nerve Paralysis Treatment and Research
- Mesenchymal stem cell research
Air Force Medical University
2015-2024
Ruijin Hospital
2018-2024
Xi'an Medical University
2024
Shanghai Jiao Tong University
2011-2024
Xijing Hospital
2014-2023
Shandong Agricultural University
2016-2023
China XD Group (China)
2016
State Key Laboratory of Microbial Technology
2004-2012
Shandong University
2004-2012
Massachusetts General Hospital
2007
The nuclear protein high-mobility group box 1 (HMGB-1) promotes inflammation in sepsis, but little is known about its role brain ischemia-induced inflammation. We report that HMGB-1 and receptors, receptor for advanced glycation end products (RAGE), Toll-like 2 (TLR2), TLR4, were expressed normal cultured neurons, endothelia, glial cells. During middle cerebral artery occlusion (MCAO), mice, immunostaining rapidly disappeared from all cells within the striatal ischemic core h after onset of...
EGF promotes proliferation and migration of stem/progenitor cells in the normal adult brain. The effect epidermal growth factor on neurogenesis ischemic brain is unknown, however. Here we show that intraventricular administration albumin augments 100-fold neuronal replacement injured mouse striatum after cerebral ischemia. Newly born immature neurons migrate into lesion differentiate mature parvalbumin-expressing neurons, replacing more than 20% interneurons lost by 13 weeks ischemia...
Calcineurin is a Ca(2+)/calmodulin-dependent protein phosphatase that abundantly expressed in several specific areas of the brain, which are exceptionally vulnerable to stroke, epilepsy, and neurodegenerative diseases. In this study, we assessed effects high level activity calcineurin on neuronal cells. Virus-mediated constitutive rendered cells susceptible apoptosis induced by serum reduction or brief exposure calcium ionophore. Adenovirus-mediated, forced cytochrome c/caspase-3-dependent...
Cell death from spinal cord injury is mediated in part by apoptotic mechanisms involving downstream caspases (e.g., caspase-3). Upstream may involve other such as procaspase-8, a 55 kDa apical caspase, which we found constitutively expressed within neurons along with Fas. As early 1.5 hr after transient ischemia, activated caspase-8 (p18) and mRNA appeared intermediate gray matter medial ventral horn. We also detected evidence for an increase receptor complex co-immunoprecipitation using Fas...
Porcine reproductive and respiratory syndrome (PRRS) is one of the most serious diseases threatening swine industry worldwide. However, no satisfactory control strategy has existed until now. In this study, effectiveness tylvalosin against PRRSV underlying mechanism was investigated. The results showed that in porcine alveolar macrophages (PAMs), can inhibit replication NADC30-like NADC34-like strains a dose-dependent manner. It worth noting pre-incubation with had significant inhibitory...
Delayed neuronal death in the hippocampal CA1 region after transient forebrain ischemia may share its underlying mechanism with neurodegeneration and other modes of death. The precise mechanism, however, remains unknown. In postischemic hippocampus, conjugated ubiquitin accumulates free is depleted, suggesting impaired proteasome function. authors measured regional activity male Mongolian gerbils. At 30 minutes ischemia, was 40% normal frontal cortex hippocampus. After 2 hours reperfusion,...
It has been reported that the degeneration of cochlear hair cells is typical cause presbycusis (or age-related hearing loss). However, molecular mechanisms mediate cell apoptosis are not yet fully understood and there no effective treatment for this disorder. MicroRNAs (miRNAs or miRs) have increasingly shown to be associated with diseases emerging as promising therapeutic targets. In study, we investigated whether miR-29b involved in cells. To examine our hypothesis, nuclear staining...
Production of N-acetyl-D-neuraminic acid (Neu5Ac) via biocatalysis is traditionally conducted using isolated enzymes or whole cells. The use restricted by the time-consuming purification process, whereas application cells limited permeability barrier presented microbial cell membrane. In this study, a novel type biocatalyst, Neu5Ac aldolase on surface Bacillus subtilis spores, was used for production Neu5Ac. Under optimal conditions, at high concentration (54.7 g liter⁻¹) and yield (90.2%)...
The aim of the present study was to explore mechanism through which miR-130a-3p affects viability, proliferation, migration, and invasion nasopharyngeal carcinoma (NPC). Tissue samples were collected from hospital department. NPC cell lines purchased conduct in vitro vivo assays. A series biological assays including MTT, Transwell, wound healing conducted investigate effects BACH2 on cells. MiR-130a-3p down-regulated both tissues lines, whereas up-regulated lines. overexpression inhibited...
In this study, the prevalence, phenotypes, and clonal relationships of
Tympanic membrane (TM) perforation is a frequent cause of conductive hearing loss. The most popular surgical repair autografting with temporalis fascia, although some disadvantages have been found this method. Whether xenogeneous grafts produced by tissue engineering approach could be used unclear. purpose study was to evaluate the possibility bioengineering TM using porcine acellular dermis and dura mater fibroblasts compare effects these two natural scaffolds. Both materials were prepared...
Spiral ganglion neuron (SGN) damage and apoptosis can lead to noise-induced hearing loss, age‑associated loss and, in certain cases, auditory neuropathy. The apoptosis‑inducing factor (AIF)‑associated pathway may be important this process. present study aimed investigate the expression levels of AIF calpain damaged SGNs. Glutamate (Glu) perfusion cell culture different concentrations Glu were performed SGNs Sprague‑Dawley (SD) rats, with saline water used as a control Different (5, 10, 20 40...
Our previous study demonstrated that intranasal administration of histone deacetylase inhibitor sodium butyrate (NaB) exhibits therapeutic effects on a mouse model allergic rhinitis (AR). However, whether NaB is effective AR when administered orally and prophylactically, as well its potential gene expression, remained unknown. The present aimed to investigate the preventive effect added diet newly weaned mice evaluate changes in long non‑coding (lnc)RNA mRNA expression profiles nasal mucosa....
Salmonella is a common food-borne Gram-negative pathogen with multiple serotypes. Pullorum disease, caused by Pullorum, seriously threatens the poultry industry. Many previous studies were focused on epidemiological characteristics of infections in conventional antibiotic use poultry. However, little known about chicken flocks fed antibiotic-free diets. Herein, we investigated and compared three Chinese native breeders diets, including Luhua, Langya, Qingjiaoma chickens, one breeder, Bairi...
Abstract The objective of this study is to evaluate the role cyclin-dependent kinase inhibitor p57 in EB1089-inhibited proliferation human laryngeal squamous carcinoma cells (HEp-2). HEp-2 were treated with vitamin D3 analogue EB1089 for 48 h and total RNA was extracted reverse transcription-PCR amplification using primers coding sequence. Proteins detected by Western blot analysis. For interference silencing (siRNA), transfected siRNA specific (siRNA-p57) or a negative control sequence...