A5102874024- Reproductive Biology and Fertility
- Animal Genetics and Reproduction
- CRISPR and Genetic Engineering
- Reproductive System and Pregnancy
- Ovarian function and disorders
- Assisted Reproductive Technology and Twin Pregnancy
- MicroRNA in disease regulation
- Neonatal Respiratory Health Research
- Animal Virus Infections Studies
- Extracellular vesicles in disease
- Pluripotent Stem Cells Research
- Cancer-related molecular mechanisms research
- Cystic Fibrosis Research Advances
- Infant Nutrition and Health
- Reproductive Physiology in Livestock
- Endometriosis Research and Treatment
- Animal Disease Management and Epidemiology
- Reproductive Health and Technologies
- Virus-based gene therapy research
- Viral gastroenteritis research and epidemiology
- Xenotransplantation and immune response
- Advanced biosensing and bioanalysis techniques
- RNA regulation and disease
- Glioma Diagnosis and Treatment
- Epigenetics and DNA Methylation
Memorial Sloan Kettering Cancer Center
2025
Henan Provincial People's Hospital
2017-2025
Zhengzhou University
2017-2025
Xuzhou Medical College
2016-2025
Sacred Heart University
2025
Ministry of Agriculture and Rural Affairs
2025
China Agricultural University
2025
Henan University
2020-2024
Henan Bioengineering Technology Research Center
2021-2023
Harbin Veterinary Research Institute
2021-2023
The CRISPR-Cas9 system is a widely utilized platform for transgenic animal production in various species, although its off-target effects should be addressed. Several applications of this tool have been proposed model animals but remain insufficient livestock production.
Infertility is a reproductive health problem which affects not only individuals, families and social populations. Recently, the infertility rate in China has trend of increase year by year, few studies have reported Henan Province, China. The aim this study was to investigate current prevalence associated factors among women childbearing age China.This cross-sectional conducted from March 2019 October 2019. We sampled 765 who were 20-49 years old eight hospitals four cities This survey...
Abstract Follicular atresia is primarily induced by granulosa cell apoptosis, but description of the apoptotic pathway in cells incomplete. In this study, we explored possibility that endoplasmic reticulum (ER) stress could be involved apoptosis during goat follicular atresia. Immunohistochemical analysis revealed DNA damage‐inducible transcript 3 (DDIT3) and glucose‐regulated protein 78 (Grp78) were observed scattered atretic follicles. Grp78 DDIT3 mRNA upregulated atresia, although was not...
Abstract β-Lactoglobulin (BLG) is a major goat’s milk allergen that absent in human milk. Engineered endonucleases, including transcription activator-like effector nucleases (TALENs) and zinc-finger nucleases, enable targeted genetic modification livestock. In this study, TALEN-mediated gene knockout followed by knock-in were used to generate BLG goats as mammary gland bioreactors for large-scale production of lactoferrin (hLF). We introduced precise modifications the goat genome at...
In vitro fertilization and embryo transfer (IVF-ET) is one of the effective methods to treat female infertility. Poor endometrial receptivity (ER) an important factor leading implantation dysfunction, which can reduce pregnancy rate IVF-ET. The mice model with dysfunction in vivo attachment trophoblast (JAR) spheroids were constructed. levels lncRNA NEAT1, HOXA10, CTCF markers ER detected. cell proliferation was measured. interaction between NEAT1 CTCF, HOXA10 promoter confirmed. LncRNA...
Introduction Porcine reproductive and respiratory syndrome virus (PRRSV), an economically significant threat to the world pork production, is notoriously known for its heterogeneity, therefore current vaccines often fail provide efficient cross-protection against diverse PRRSV strains. Methods By making chimeric viruses using HP-PRRSV-2 lineage 8 (JXwn06) 1 NADC30-like strains (CHsx1401) as model organisms, recently results have shown that viral structural protein-coding region critical...
The study was designed to analyze early pregnancy loss rates in first-time fresh embryo transfer cycles low prognosis patients according the POSEIDON criteria. This a retrospective cohort study, including with positive human chorionic gonadotropin after first Reproductive Center of Henan Province People's Hospital from June 2018 February 2023. A total 2392 were included this which divided into 4 groups general condition, laboratory indexes, and compared each group prediction model...
ABSTRACT Introduction Left atrium epicardial adipose tissue (LA‐EAT) is an atrial cardiomyopathy marker associated with ischemic stroke. However, the relationship between EAT and silent cerebral infarcts (SCI) unclear. This study investigated effect of on risk SCI after fibrillation catheter ablation (AFCA). Methods was a single‐center prospective study. We consecutively enrolled patients who underwent AFCA from October 2019 to February 2024. All completed brain magnetic resonance imaging...
Abstract Background Cystic fibrosis (CF) is an autosomal recessive disorder rarely found in Asian populations. Most males with CF are infertile because of obstructive azoospermia (OA) caused by congenital bilateral absence the vas deferens (CBAVD). Compound heterozygous mutations cystic transmembrane conductance regulator ( CFTR ) among most common pathogenic factors CBAVD. However, few genealogical analyses have been performed. Methods In this study, whole‐exome sequencing and cosegregation...
This study aimed to evaluate the specific regulatory roles of microRNA-146a (miRNA-146a) in temporal lobe epilepsy (TLE) and explore related mechanisms. A rat model TLE was established by intraperitoneal injection lithium chloride-pilocarpine. These rats were injected intracerebroventricularly with an miRNA-146a inhibitor Notch-1 siRNA. Then, neuronal damage cell apoptosis cornu ammonis (CA) 1 3 regions hippocampus assessed. SOD MDA levels detected chromatometry, IL-1β, IL-6, IL-18 ELISA. we...
Luman/CREB3 recruitment factor (LRF or CREBRF) was identified as a regulator of Luman (or CREB3) that is involved in the unfolded protein response during endoplasmic reticulum stress. implicated multitude functions ranging from viral infection and immunity to cancer. The biological function LRF, however, unknown. In this paper, we report uteri pregnant mice embryos displayed enhanced LRF expression at all stages, expressed found be localized specifically implantation sites. On other hand,...
Abstract Targeting exogenous genes at milk protein loci via gene-targeting technology is an ideal strategy for producing large quantities of pharmaceutical proteins. Transcription- activator-like effector (TALE) nucleases (TALENs) are efficient genome-editing tool. However, the off-target effects may lead to unintended gene mutations. In this study, we constructed TALENs and TALE nickases directed against exon 2 bovine β-lactoglobulin (BLG) locus. The can induce a site-specific DNA...
Vitamin C, an antioxidant that reduces reactive oxygen species (ROS) in cells, is capable of significantly improving the developmental competence porcine and mouse somatic cell nuclear transfer (SCNT) embryos, both vitro vivo. In present study, effects vitamin C on bovine SCNT embryos were investigated. The results indicated (40 μg/mL) positively affected scavenging intracellular ROS, cleavage rate at 24 h (76.67 vs. 68.26%, p<0.05), compact morulae formation (60.83 51.30%, blastomere...
Non-obstructive azoospermia (NOA) is a common cause of male infertility, and genetic problems, such as chromosomal abnormalities gene mutations, are important causes NOA. Our centre received case NOA, in which no mature sperm was found during microdissection testicular extraction. A postoperative pathological examination revealed that spermatogenesis blocked. Target region capture combined with high-throughput sequencing used to screen for infertility-related mutations. Sanger further...
To investigate the expression of high-mobility group box 1 (HMGB1) in human dental pulp tissues and effects HMGB1 on proliferation odontoblastic differentiation cells (hDPCs).Immunohistochemical assay, immunofluorescence staining flow cytometric analysis were used to detect hDPCs, respectively. The hDPCs was examined by CCK-8 after culturing primary presence with different doses. Odontoblastic determined using alkaline phosphatase (ALPase) activity assay mineralized nodule formation....
Objectives Assisted reproductive technology (ART) is an important part of medicine, whose possible effects on offspring’s health have drawn widespread attention in recent years. However, relevant studies are limited to postnatal short-term follow-up and lack diverse sample sources analysis other than blood. Methods In this study, a mouse model was used explore the ART fetal development gene expression organs offspring adulthood using next-generation sequencing. The sequencing results were...
In this article, the expression patterns of LRF in mouse oviduct, uterus, and ovary were checked during estrous cycle. The mRNA protein remarkably changed ovary, uterus at four phases. immunostaining was detected follicle from primary to antral follicle, luminal glandular epithelial cells cilia ciliated oviduct all phase. Our findings suggested that may be related processes development maturation oocyte, gamete transport, early embryo.