A5090374117- Reproductive Biology and Fertility
- Animal Genetics and Reproduction
- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Composting and Vermicomposting Techniques
- Sperm and Testicular Function
- Renal and related cancers
- Extracellular vesicles in disease
- Virus-based gene therapy research
- Reproductive Physiology in Livestock
- Epigenetics and DNA Methylation
- Reproductive System and Pregnancy
- Genetic and phenotypic traits in livestock
- Tissue Engineering and Regenerative Medicine
- Pregnancy and preeclampsia studies
- Wastewater Treatment and Nitrogen Removal
- RNA Interference and Gene Delivery
- MicroRNA in disease regulation
- Microbial Community Ecology and Physiology
- Biochemical Acid Research Studies
- Biofuel production and bioconversion
- Cancer-related molecular mechanisms research
- Muscle Physiology and Disorders
- Pharmaceutical and Antibiotic Environmental Impacts
- Reproductive Health and Technologies
Ministry of Agriculture and Rural Affairs
2023-2024
Northwest A&F University
2014-2024
Agriculture and Forestry University
2023-2024
BGI Group (China)
2024
BGI Research
2024
Ministry of Agriculture, Livestock, and Food Supply
2012-2023
Jilin University
2015
Ministry of Agriculture and Agro Based Industry
2015
Ministry of Agriculture & Farmers Welfare
2015
Chinese Academy of Medical Sciences & Peking Union Medical College
2010-2011
Maternal effect genes encode proteins that are produced during oogenesis and play an essential role early embryogenesis. Genetic ablation of such in oocytes can result female subfertility or infertility. Here we report a newly identified maternal gene, Nlrp2, which plays embryogenesis the mouse. Nlrp2 mRNAs their (∼118 KDa) expressed granulosa cells folliculogenesis. The transcripts show striking decline preimplantation embryos before zygotic genome activation, but remain present through to...
Mastitis costs the dairy industry billions of dollars annually and is most consequential disease cattle. Transgenic cows secreting an antimicrobial peptide demonstrated resistance to mastitis. The combination somatic cell gene targeting nuclear transfer provides a powerful method produce transgenic animals. Recent studies found that precisely placed double-strand break induced by engineered zinc-finger nucleases (ZFNs) stimulated integration exogenous DNA stretches into pre-determined...
Zinc-finger nickases (ZFNickases) are a type of programmable nuclease that can be engineered from zinc-finger nucleases to induce site-specific single-strand breaks or nicks in genomic DNA, which result homology-directed repair. Although nuclease-mediated gene disruption has been demonstrated pigs and cattle, they have not used target addition into an endogenous locus any large domestic species. Here we show bovine fetal fibroblasts targeting ZFNickases the β-casein (CSN2) stimulates...
We previously found that treatment of both donor cells and early cloned embryos with combination 5-aza-2'-deoxycytidine (5-aza-dC) trichostatin A (TSA) significantly improve the in vitro full-term development nuclear transfer (NT) bovine embryos. To investigate how this improved epigenetic reprogramming somatic cell nuclei, we compared expression levels DNA methylation-, chromatin structure-, development-related genes fertilized (IVF group), NT (C-NT 5-aza-dC TSA-treated (T-NT group) single...
Aberrant epigenetic nuclear reprogramming results in low somatic cloning efficiency. Altering status by applying histone deacetylase inhibitors (HDACi) enhances developmental potential of cell transfer (SCNT) embryos. The present study was carried out to examine the effects Oxamflatin, a novel HDACi, on and development bovine SCNT embryos vitro. We found that Oxamflatin modified acetylation H3K9 H3K18, increased total inner mass (ICM) numbers ratio ICM∶trophectoderm (TE) cells, reduced rate...
The selection of good quality oocytes is crucial for in vitro fertilization and somatic cloning. Brilliant cresyl blue (BCB) staining has been used from several mammalian species. However, the effects differential oocyte by BCB on nuclear reprogramming vivo development SCNT embryos are not well understood. Immature compact cumulus–oocyte complexes (COCs) were divided into control (not exposed to BCB), BCB+ (blue cytoplasm) BCB− (colorless groups. We found that yielded a significantly higher...
Melatonin is present in mammalian follicular fluid and plays an important role regulating steroidogenesis cells. In this study, we report the effect of melatonin on theca interna (TI) small bovine follicles cells (TCs) cultured vitro. Treatment with significantly increased expression steroidogenic acute regulatory protein (STAR) production progesterone both TI TCs. stimulated phosphorylation AKT but not ERK1/2, addition luzindole (a nonspecific MT1 MT2 inhibitor) or 4P-PDOT (specific reduced...
Abstract Resveratrol (Res) has been reported to be able improve oocyte vitrification because of its antioxidative properties. The objective this study was further assess the positive effect Res addition on developmental potential vitrified mouse oocytes from perspective epigenetic alterations. First, 2 μM chosen as optimal concentration basis effects survival and We found that significantly promoted fertilization (63.8% vs. 42.9%) blastocyst formation (68.3% 50.2%) after vitrification....
Abstract As an assisted reproduction technology, vitrification has been widely used for oocyte and embryo cryopreservation. Many studies have indicated that affects ultrastructure, gene expression, epigenetic status. However, it is still controversial whether could induce DNA damage in metaphase II (MII) oocytes the resulting early embryos. This study determined mouse MII preimplantation embryos, causes vitrification‐induced damage. The effects of on reactive oxygen species (ROS) levels,...
Porcine circovirus type 3 is a newly emerging pathogen of porcine associated disease (PCVAD). Currently, there no commercially available vaccine, resulting in huge economic losses to the pig industry. capsid protein (Cap) can self-assemble into virus-like particles (VLPs). Therefore, expression recombinant Cap great significance for prevention, diagnosis and control diseases. In this study, was successfully expressed Escherichia coli by deleting nuclear localization sequence (NLS). The VLPs...
Antibiotic selectable marker genes have been widely used to generate transgenic animals. Once animals obtained, the is no longer necessary but raises public concerns regarding biological safety. The aim of this study was prepare competent antibiotic free cells for somatic cell nuclear transfer (SCNT). PhiC31 intergrase insert a transgene cassette into "safe harbor" in bovine genome. Then, Cre recombinase employed excise under monitoring fluorescent double reporter. By visually tracking...
Contents Early follicular development is closely related to oocyte‐granulosa cells‐ovarian stromal cells/theca cells. The aim of the present study was investigate effects ovarian cortical, medullary and theca cells on oestradiol progesterone biosynthesis, proliferation apoptosis goat ovary granulosa in vitro . Using T ranswell coculture system, we evaluated steroidogenesis, cell apoptosis, some molecular expressions regarding steroidogenic enzyme, luteinizing hormone receptor...