Abstract Background MicroRNA-200c (miR-200c) is one of the short noncoding RNAs that play crucial roles in tumorigenesis and tumor progression. It also acts as considerable modulator process epithelial-to-mesenchymal transition (EMT), a cell development regulating affects metastasis. However, role miR-200c bladder cancer cells its mechanism has not been well studied. The purpose this study was to determine potential EMT how it contributed invasion, migration proliferation. Methods Real-time...

Abstract Reactive oxygen species (ROS) and cellular oxidant stress are regulators of cancer cells. The alteration redox status, which is induced by increased generation ROS, results in vulnerability to oxidative stress. aim this study investigate the influence O2-(2,4-dinitrophenyl) 1-[(4-ethoxycarbonyl)piperazin-1-yl]diazen-1-ium-1,2-diolate (JS-K, C 13 H 16 N 6 O 8 ) on proliferation apoptosis bladder cells explored possible ROS-related mechanisms. Our indicated that JS-K could suppress...

Isoflavones, a group of soybean nutrients, have been found to provide wide range benefits the human body. The prospective cohort study aims examine association dietary intake isoflavones with all-cause mortality and heart disease in US. In this study, We conducted explore potential correlation (total isoflavones, genistein, daidzein) risk mortality. status cause death were determined by NHANES-linked National Death Index public access files through December 31, 2019. was analyzed...

Hepatocellular carcinoma (HCC) is a life-threatening disease that known to exhibit poor prognosis. Therefore, it important identify an effective drug therapy for the treatment of HCC. Dihydromyricetin (DHM) flavonoid compound, isolated from classical Chinese herb Ampelopsis grossedentata, which exhibits multiple pharmacological activities, including anticancer effects. In this study, effect DHM was investigated in nine different types HCC cell lines via proliferation and immunoassays, as...

Contents Early follicular development is closely related to oocyte‐granulosa cells‐ovarian stromal cells/theca cells. The aim of the present study was investigate effects ovarian cortical, medullary and theca cells on oestradiol progesterone biosynthesis, proliferation apoptosis goat ovary granulosa in vitro . Using T ranswell coculture system, we evaluated steroidogenesis, cell apoptosis, some molecular expressions regarding steroidogenic enzyme, luteinizing hormone receptor...

In view of the fact that JS-K might regulate ubiquitin E3 ligase and plays an important role in mechanism CRPC formation, goal was to investigate probable by which regulates prostate cancer cells. Proliferation inhibition on cells examined usingCCK-8 assays. Caspase 3/7 activity assays flow cytometry were performed examine whether induced apoptosis Western blotting co-immunoprecipitation analyses investigated JS-K's effects associated mechanism. Real time-PCR assess effect transcription...

Reactive oxygen species (ROS) are chemical that alter redox status, and responsible for inducing carcinogenesis. The purpose of the present study was to assess effects glutathione S transferase-activated nitric oxide donor prodrug, JS-K, on ROS accumulation proliferation apoptosis in human prostate cancer cells. Cell apoptosis, activation mitochondrial signaling pathway were measured. results demonstrated JS-K may inhibit cell growth a dose- time-dependent manner, induce dose-dependent...

Novel stem cells expressing stage-specific embryonic antigen 3 (SSEA-3) reside among human dermal fibroblasts and are known as multilineage-differentiating stress-enduring (Muse) cells. They enhance the generation efficiency of induced pluripotent However, Muse have only been found in humans. We aimed to isolate SSEA3-positive from terminally differentiated skin adult goat determine their pluripotency. Cell clusters SSEA3+ populations possessed cell-like morphological features normal...

Background: Diosmetin (DIOS) is the aglycone of flavonoid glycoside, diosmin, derived naturally from leaves legume, Olea europaea, and Acacia farnesiana. It has potent anticancer activity against multiple forms cancers. However, role DIOS in renal carcinoma its mechanism action remain unclear. Objective: The purpose this study to investigate effect on cell viability apoptosis cells explore possible action. Methods: Cell viability, cytotoxicity, caspase activity, apoptosis, expression...

The aim of the present study was to evaluate functions miR‑200c in regulation tumor growth and metastasis renal cancer cells, investigate underlying mechanisms. In this study, up- downregulated two cell lines, namely ACHN A498, proliferation, colony formation, migration invasion cells were measured. expression levels various mRNAs proteins then analyzed using reverse transcription-quantitative polymerase chain reaction western blotting, respectively. It found that suppressed and, conversely,...

Background: Ovarian cancer is the most lethal gynecologic malignancy worldwide with poor prognosis owing to chemotherapy resistance and relapse. Hence, there an urgent need develop novel anticancer agents against ovarian cancer. Objective: The aim of this research investigate possible activity aloperine, active ingredient from a traditional Chinese medicine Sophora alopecuroides, explore Reactive Oxygen Species (ROS)-related mechanism. Methods: Cell viability, cytotoxicity, apoptosis, ROS...

The aim of the present study was to investigate effect resveratrol on renal carcinoma cells and explore possible renin-angiotensin system-associated mechanisms. Subsequent treatment, cell viability, apoptosis rate, cytotoxicity levels, caspase 3/7 activity levels angiotensin II (AngII), AngII type 1 receptor (AT1R), vascular endothelial growth factor (VEGF) cyclooxygenase-2 (COX-2) were evaluated in cells. effects AngII, AT1R, VEGF COX-2 resveratrol-induced inhibition also examined. results...

The aim of the present study was to investigate influence nitric oxide donor prodrug JS-K (C13H16N6O8) on Taxol-induced apoptosis in prostate cancer cells, and a potential reactive oxygen species (ROS)-associated mechanism. effect anticancer activity Taxol assessed cells; cell viability, colony formation, apoptosis, ROS generation expression levels apoptosis-associated proteins were investigated. function accumulation combined effects determined using antioxidant N-acetylcysteine (NAC)...

Previous studies have demonstrated that skin stem cells expressing the pluripotency marker stage-specific embryonic antigen 3 (SSEA3) are easier to reprogram into induced pluripotent (iPSCs) than fibroblasts. Furthermore, it is widely speculated undifferentiated state may make more efficient donor for somatic cell nuclear transfer (SCNT). In this study, we isolated SSEA3(+) from goat fibroblast (SFCs) using fluorescence-activated sorting (FACS) and examined expression of markers in vitro...

Aloperine (ALO) is an important active component of quinolizidine alkaloids in Sophora flavescens A and alopecuroides L, has effective anticancer activity against multiple cancers. However, the influence mechanism ALO on migration, invasion, adhesion bladder cancer cells remain unclear.The aim this study to determine effect investigate its potential TIMP-4-related mechanism.Cell viability, cytotoxicity, wound healing, Transwell cell adhesion, real-time qPCR, western blot, ELISA assays were...