A5090102572- Cervical Cancer and HPV Research
- Head and Neck Cancer Studies
- T-cell and Retrovirus Studies
- Animal Disease Management and Epidemiology
- Vector-Borne Animal Diseases
- Head and Neck Surgical Oncology
- Nonmelanoma Skin Cancer Studies
- Genital Health and Disease
- Oral Health Pathology and Treatment
- Immunotherapy and Immune Responses
- Hearing, Cochlea, Tinnitus, Genetics
- Ear Surgery and Otitis Media
- Colorectal and Anal Carcinomas
- Virus-based gene therapy research
- Chromatin Remodeling and Cancer
- Autoimmune Bullous Skin Diseases
- Cancer Cells and Metastasis
- Polyomavirus and related diseases
- Viral-associated cancers and disorders
- Cancer and Skin Lesions
- Facial Nerve Paralysis Treatment and Research
- Meningioma and schwannoma management
- Cutaneous Melanoma Detection and Management
- Infectious Diseases and Mycology
- Ear and Head Tumors
National University Health System
2022-2024
National University of Singapore
2022-2024
National University Hospital
2022-2024
Klinikum Bielefeld
2021-2023
Bielefeld University
2022-2023
Universitätsklinik für Hals-, Nasen- und Ohrenheilkunde
2023
Peterborough City Hospital
2020-2021
Addenbrooke's Hospital
2007-2020
Norfolk and Norwich University Hospital
2014-2018
University of Cambridge
2009-2016
Cell contact is required for efficient transmission of human T cell leukemia virus– type 1 (HTLV-I) between cells and individuals, because naturally infected lymphocytes produce virtually no cell-free infectious HTLV-I particles. However, the mechanism cell-to-cell spread not understood. We show here that rapidly induces polarization cytoskeleton to cell-cell junction. core (Gag protein) complexes genome accumulate at junction are then transferred uninfected cell. Other lymphotropic viruses,...
No therapies have been proven to persistently improve the outcome of HTLV-I-associated myelopathy. Clinical benefit has reported with zidovudine and lamivudine in observational studies. We therefore conducted a randomised, double blind, placebo controlled study six months combination therapy these nucleoside analogues sixteen patients. Primary outcomes were change HTLV-I proviral load PBMCs clinical measures. Secondary endpoints changes T-cell subsets markers activation proliferation. Six...
Abstract Condylomata acuminata (genital warts) are the most common sexually transmitted viral diseases. These lesions caused by infection with mucosal human papillomaviruses (HPVs). However, there is limited information on HPV strain distribution involved in molecular pathogenesis of these lesions. To address this, prevalence and frequency multiple infections were determined wart tissue obtained from 31 patients attending a clinic. bisected subjected to parallel DNA mRNA extractions....
Abstract Background The low-risk human papillomavirus types 6 and 11 are responsible for approximately 90% of anogenital wart cases, with 190,000 new recurrent cases reported in the UK 2010. has recently selected quadrivalent HPV vaccine, which conveys protection against both HPV6 11, as part its immunisation programme 2012 it is expected that this will reduce disease burden UK. aims study were to evaluate current strategies used monitoring infection genital warts assess suitability...
This study was designed to identify significant differences in gene expression profiles of human papillomavirus ( HPV )‐positive and ‐negative oropharyngeal squamous cell carcinomas OPSCC ) better understand the functional biological effects infection premalignant pathway. Twenty‐four consecutive patients with locally advanced primary were included a prospective clinical trial. Fresh tissue samples (tumor vs . matched normal epithelium) subjected whole transcriptome analysis results...
The proviral load in human T cell lymphotropic virus type 1 (HTLV-1) infection is typically constant each infected host, but varies by >1000-fold between hosts and strongly correlated with the risk of HTLV-1-associated inflammatory disease. However, factors that determine an individual's HTLV-1 remain uncertain. Experimental evidence from studies host genetics, viral lymphocyte function theoretical considerations suggest a major determinant equilibrium CD8+ response to HTLV-1. In this study,...
The CD8+ lymphocyte response is a main component of host immunity, yet it difficult to quantify its contribution the control persistent viruses. Consequently, remains controversial as whether cells have biologically significant impact on viral burden and disease progression in infections such human immunodeficiency virus-1 T-lymphotropic virus type I (HTLV-I). Experiments ascertain based cell frequency or specificity alone give inconsistent results. Here, an alternative approach was...
Human T cell lymphotropic virus type1(HTLV-1)causes HTLV-l-associated myelopathy/tropical spastic paraparesis (HAM/TSP). We used interferon- enzyme-linked immunospot assays with overlapping peptides spanning the entire HTLV-1proteome to test whether HTLV-l-specific CD8+ cells differed significantly in frequency or immunodominance hierarchy between patients HAM/TSP and asymptomatic carriers correlated provirus load. Tax was immunodominant target antigen. There no significant qualitative...
Abstract CD4+ T cells predominate in early lesions the CNS inflammatory disease human lymphotropic cell virus type I (HTLV-I)-associated myelopathy/tropical spastic paraparesis (HAM/TSP), but pathogenesis of remains unclear and HTLV-I-specific response has been little studied. We quantified IFN-γ-producing cells, patients with HAM/TSP asymptomatic carriers high proviral load, to test two hypotheses: that HTLV-I a similar load differ immunodominance hierarchy or total frequency specific are...
Human papillomaviruses (HPV) are causally associated with ano-genital and a subset of head neck cancers. Rising incidence HPV+ anal cancers have now been demonstrated in the developed world over last decade. The majority published data on HPV prevalence at oro-pharyngeal sites from studies higher-risk populations. There is paucity non-cervical lower risk, non-HIV+ women this study was designed to provide initial pilot population recalled for colposcopy as part UK cervical screening...
Recurrent respiratory papillomatosis (RRP) is a rare disease, but one with severe morbidity and occasional mortality. The aetiological agent human papillomavirus (HPV), HPV types 6 11 account for over 90% of all cases. In the active phase patients require multiple hospital admissions surgical removal or ablation these benign tumors, which are likely to obstruct airways if left unchecked. Long-term sequelae include scarring vocal cords, change in voice timbre, even muteness tracheostomy...
New concepts for a more effective anti-cancer therapy are urgently needed. Experimental flaws represent major counter player of this development and lead to inaccurate unreproducible data as well unsuccessful translation research approaches into clinics. In previous study we have created epithelial cell cultures from head neck squamous carcinoma (HNSCC) tissue.We characterize primary populations isolated human papillomavirus positive HNSCC tissue their marker expression by RT-qPCR, flow...
Recurrent Respiratory Papillomatosis(RRP) is a rare disease with severe morbidity. Treatment surgical. Prevailing viewpoint that prophylactic HPV vaccines do not have therapeutic benefit due to their modus operandi. Studies on vaccination alongside surgery were meta-analysed test effect burden of disease. Databases accessed Nov and Dec 2021 [PubMed, Cochrane, Embase Web Science]. Main outcome measured was: Mean paired differences in the number surgeries or recurrences per month. Analyses was...
Significantly higher frequencies of tumor necrosis factor alpha- and interleukin-2-secreting human T-lymphotropic virus type 1 (HTLV-1)-specific CD4(+) T cells were present in the peripheral blood mononuclear HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP) patients than those asymptomatic carriers with similar provirus loads. The data suggest that HTLV-1-specific play a role pathogenesis HAM/TSP.
Abstract Background Human papillomaviruses (HPV) are the aetiological agents of certain benign and malignant tumours skin mucosae; most important which is cervical cancer. Also, incidence ano-genital warts, HPV-anal cancer oropharyngeal cancers rising. To help ascertain a useful PCR detection protocol for cancers, we directly compared three commonly used primer sets in HPV from different clinical samples. Methods We PGMY09/11, MY09/11 GP5+/6+ primers PCRs 34 clinically diagnosed samples...