A5011429882- Glioma Diagnosis and Treatment
- Intracranial Aneurysms: Treatment and Complications
- Cancer Cells and Metastasis
- Virus-based gene therapy research
- Epilepsy research and treatment
- Neuroblastoma Research and Treatments
- Head and Neck Cancer Studies
- Neurological disorders and treatments
- Chromatin Remodeling and Cancer
- Head and Neck Surgical Oncology
- Spine and Intervertebral Disc Pathology
- Neuroscience and Neuropharmacology Research
- CRISPR and Genetic Engineering
- Cerebrovascular and Carotid Artery Diseases
- Pancreatic and Hepatic Oncology Research
- Ear Surgery and Otitis Media
- Protein Degradation and Inhibitors
- Trigeminal Neuralgia and Treatments
- Cancer Genomics and Diagnostics
- Cerebrospinal fluid and hydrocephalus
- Epigenetics and DNA Methylation
- Meningioma and schwannoma management
- Immunotherapy and Immune Responses
- Neuroscience and Neural Engineering
- Brain Metastases and Treatment
Klinikum Bielefeld
1990-2024
Bielefeld University
2021-2024
Dana-Farber Cancer Institute
2016-2022
Harvard University
2019-2022
German Cancer Research Center
2012-2019
Heidelberg University
2012-2019
National Center for Tumor Diseases
2013-2019
University Hospital Carl Gustav Carus
2011
TU Dresden
2011
Evangelisches Krankenhaus Bielefeld
1998-2009
Retinoic acid reprograms neuroblastoma cell identity by remodeling the core transcriptional regulatory circuitry.
Abstract Background SOX2, a high mobility group (HMG)-box containing transcription factor, is key regulator during development of the nervous system and persistent marker neural stem cells. Recent studies suggested role SOX2 in tumor progression. In our previous work we detected glioma cells glioblastoma specimens. Herein, aim to explore for malignancy particular its cell proliferation migration. Methods Retroviral shRNA-vectors were utilized stably knockdown U343-MG U373-MG The resulting...
The SWI/SNF-family chromatin remodeling protein ATRX is a tumor suppressor in sarcomas, gliomas and other malignancies. Its loss of function facilitates the alternative lengthening telomeres (ALT) pathway cells, while it also affects Polycomb repressive complex 2 (PRC2) silencing its target genes. To further define role inactivating mutations carcinogenesis, we knocked out atrx our previously reported p53/nf1-deficient zebrafish line that develops malignant peripheral nerve sheath tumors...
Abstract Although tumor‐initiating cell ( TIC ) self‐renewal has been postulated to be essential in progression and metastasis formation of human pancreatic adenocarcinoma PDAC ), clonal dynamics s within tumors are yet unknown. Here, we show that long‐term serial xenotransplantation is driven by a succession transiently active producing tumor cells temporally restricted bursts. Clonal tracking individual, genetically marked revealed individual generated distinct sets with very little...
New concepts for a more effective anti-cancer therapy are urgently needed. Experimental flaws represent major counter player of this development and lead to inaccurate unreproducible data as well unsuccessful translation research approaches into clinics. In previous study we have created epithelial cell cultures from head neck squamous carcinoma (HNSCC) tissue.We characterize primary populations isolated human papillomavirus positive HNSCC tissue their marker expression by RT-qPCR, flow...
Recurrent Respiratory Papillomatosis(RRP) is a rare disease with severe morbidity. Treatment surgical. Prevailing viewpoint that prophylactic HPV vaccines do not have therapeutic benefit due to their modus operandi. Studies on vaccination alongside surgery were meta-analysed test effect burden of disease. Databases accessed Nov and Dec 2021 [PubMed, Cochrane, Embase Web Science]. Main outcome measured was: Mean paired differences in the number surgeries or recurrences per month. Analyses was...
Head and neck squamous cell carcinoma present a high mortality rate. Melatonin has been shown to have oncostatic effects in different types of cancers. However, inconsistent results reported for vivo applications. Consequently, an alternative administration route is needed improve bioavailability establish the optimal dosage melatonin cancer treatment. On other hand, use patient-derived tumor models transformed field drug research because they reflect heterogeneity patient tissues. In study,...
Patient-derived cancer xenografts (PDX) are widely used to identify and evaluate novel therapeutic targets, test approaches in preclinical mouse avatar trials. Despite their widespread use, potential caveats of PDX models remain considerably underappreciated. Here, we demonstrate that EBV-associated B-lymphoproliferations frequently develop following xenotransplantation human colorectal pancreatic carcinomas highly immunodeficient NOD.Cg-Prkdcscid Il2rgtm1Wjl /SzJ (NSG) mice (18/47 4/37...
The poor prognosis of locally advanced and metastatic head neck squamous cell carcinoma (HNSCC) is primarily mediated by the functional properties cancer stem cells (CSCs) resistance to chemoradiotherapy. We investigated whether aldehyde dehydrogenase (ALDH) inhibitor disulfiram (DSF) can enhance sensitivity therapy. Cell viability was assessed 1-(4,5-dimethylthiazol-2-yl)-3,5-diphenylformazan (MTT) apoptosis assays, cycle reactive oxygen species (ROS) levels were evaluated...
Chronic rhinosinusitis (CRS) is marked by an inflamed mucosa of sinuses and accompanied a significantly reduced quality live. Since no guidelines for the treatment CRS are available, long lasting clinical histories with health care costs adding up to dozens billion $ annually caused CRS. The progression often induced bacterial infections and/or shift in microbiome as well biofilm formation. exact alterations still unclear impenetrable renders common antibiotics ineffective. This study...
Head and neck squamous cell carcinoma is a highly malignant disease research needed to find new therapeutic approaches. Faithful experimental models are required for this purpose. Here, we describe the specific culture conditions enabling efficient establishment of primary models. Whereas classical 10% serum-containing medium resulted in growth fibroblast-like cells that outcompeted epithelial cells, found use enabled tumor from HPV+ HPV- head cancer tissue applicable research. EpCAM high...
The aim of this study was to evaluate the short- and long-term seizure outcome find predictors after epilepsy surgery in lesional posterior cortical epilepsies (PCEs).The operative 80 consecutive adult patients with PCEs who underwent resective for intractable partial between 1991 2006 retrospectively studied.The probability remaining Engel Class I 66.3% (95% CI 60 72) at 6 months, 52.5% 47 57) 2 years, 52.9% (CI 45 59) 5 years 47.1% 42 52) 10 years. Factors predicting poor were presence a...
Malignant peripheral nerve sheath tumors (MPNSTs) are soft-tissue sarcomas that frequently arise in patients with neurofibromatosis type 1 (NF1). Most of these unresectable at diagnosis and minimally responsive to conventional treatment, lending urgency the identification new pathway dependencies drugs potent antitumor activities. We therefore examined a series candidate agents for their ability induce apoptosis MPNST cells arising nf1/tp53-deficient zebrafish. In this study, we found DNA...
Cholesteatoma is a severe non-cancerous lesion of the middle ear characterized by massive inflammation, tissue destruction, and an abnormal growth keratinized squamous epithelium. We recently demonstrated presence pathogenic stem cells within cholesteatoma tissue, unfortunately their potential roles in regulating disease-specific chronic inflammation remain poorly understood. In presented study, we utilized our established human vitro cell model for treatments with lipopolysaccharides (LPS),...
Cholesteatoma disease is an expanding lesion in the middle ear. Hearing loss and facial paralysis alongside with other intracranial complications are found. No pharmaceutical treatment available today recurrence after surgical extraction occurs. We investigated possible TLR4-based mechanisms promoting explore treatments strategies.We isolated fibroblasts epidermal stem cells from cholesteatoma tissue healthy auditory canal skin. Subsequently, their expression under standard culture...
Objective: Eustachian tube function is of central importance for the ventilation middle ear. A dysfunction can be associated with chronic otitis media, and cholesteatoma. Balloon dilatation (BET) a treatment option used to solve eustachian dysfunction. Although BET widely performed, little known about occurrence rate complications BET. The aim present study was observe sensorineural hearing loss (SNHL) after Methods: We retrospectively evaluated in chart review 1,547 patients 2,614...
The quality of outcome after severe closed head injury has become increasing concern to neurosurgeons. assessment residual deficits in patients who have recovered from can be very difficult. Many are classified as having a good recovery according the Glasgow Outcome Scale (GOS), but this may insufficiently focused or sensitive demonstrate mental objectively. We investigated 33 with subsequently were diagnosed made GOS. severity was determined by Coma (GCS) and presence midline shift...