A5052627190- Electrospun Nanofibers in Biomedical Applications
- Tissue Engineering and Regenerative Medicine
- Nerve injury and regeneration
- Wound Healing and Treatments
- Mesenchymal stem cell research
- 3D Printing in Biomedical Research
- Periodontal Regeneration and Treatments
- Bone Tissue Engineering Materials
- Corneal Surgery and Treatments
- Hemostasis and retained surgical items
- Orthopedic Surgery and Rehabilitation
- Hydrogels: synthesis, properties, applications
- Osteoarthritis Treatment and Mechanisms
- Silk-based biomaterials and applications
- Tendon Structure and Treatment
- Cardiomyopathy and Myosin Studies
- Proteoglycans and glycosaminoglycans research
- Cleft Lip and Palate Research
- Molecular Biology Techniques and Applications
- Educational Technology in Learning
- Connective tissue disorders research
- Neurogenesis and neuroplasticity mechanisms
- Ocular Surface and Contact Lens
- Innovations in Medical Education
- Cardiovascular Effects of Exercise
Universidad de Granada
2015-2024
Instituto de Investigación Biosanitaria de Granada
2015-2024
Ghent University
2014-2017
University of Valparaíso
2007-2014
Stomatology Hospital
2012
Head and neck squamous cell carcinoma (HNSCC) clearly involves activation of the Akt mammalian target rapamycin (mTOR) signalling pathway. However, effectiveness treatment with mTOR inhibitor is often limited by chemoresistance. Melatonin suppresses neoplastic growth via different mechanisms in a variety tumours. In this study, we aimed to elucidate effects melatonin on rapamycin-induced HNSCC death identify potential cross-talk pathways. We analysed dose-dependent rapamycin-treated lines...
Objective.The objective was to study the effectiveness of a commercially available collagen conduit filled with fibrin-agarose hydrogels alone or containing autologous adipose-derived mesenchymal stem cells (ADMSCs) in rat sciatic nerve injury model.Approach.A 10 mm gap created 48 rats and repaired using saline-filled conduits (acellular conduits) ADMSCs (ADMSC conduits).Nerve regeneration assessed clinical, electrophysiological histological studies.Main results.Clinical outcomes were more...
Pseudomonas putida strains are ubiquitous in soil and water but have also been reported as opportunistic human pathogens capable of causing nosocomial infections. In this study we describe the multilocus sequence typing four P. (HB13667, HB8234, HB4184, HB3267) isolated from in-patients at Besançon Hospital (France). The isolates (in particular were resistant to a number antibiotics. pathogenicity virulence potential was tested ex vivo using different biological models: tissue culture,...
Advances in the development of cornea substitutes by tissue engineering techniques have focused on use decellularized scaffolds. In this work, we evaluated different chemical and physical decellularization methods small intestine tissues to determine most appropriate protocols for corneal applications. Our results revealed that efficient agents were SDS triton X-100 detergents, which able efficiently remove cell nuclei residual DNA. Histological histochemical analyses collagen fibers...
Nerve conduits are promising alternatives for repairing nerve gaps; they provide a close microenvironment that supports regeneration. In this sense, histological analysis of axonal growth is determinant to achieve successful To evaluate process, the most-used immunohistochemical markers neurofilament (NF), β-III tubulin and, infrequently, GAP-43. However, GAP-43 expression in long-term regeneration models still poorly understood. study we analysed and its correlation with NF S-100, using...
Desmin (DES) mutations cause severe skeletal and cardiac muscle disease with heterogeneous phenotypes. Recently, DES were described in patients inherited arrhythmogenic right ventricular cardiomyopathy/dysplasia, although their cellular molecular pathomechanisms are not precisely known. Our aim is to describe clinically functionally the novel DES-p.Glu401Asp mutation as a of left cardiomyopathy/dysplasia.We identified p.Glu401Asp large Spanish family cardiomyopathy/dysplasia high incidence...
Development of human skin substitutes by tissue engineering may offer new therapeutic alternatives to the use autologous grafts. For that reason, it is necessary investigate and develop biocompatible biomaterials support generation a proper construct. In this study, we generated novel model bioengineered substitute using cells obtained from biopsies fibrin-agarose evaluated both at ex vivo in levels. Once dermal fibroblasts epithelial keratinocytes were isolated expanded culture, used...
For the histopathologic diagnosis of melanocytic lesions, it could be necessary to identify melanin pigment because its visualization is unspecific with hematoxylin-eosin (HE). The Fontana-Masson (FM) technique used in histopathology this type lesion, which allows identification pigment, but loses all morphologic parameters. authors describe a modification FM method, for evaluation morphology, argentaffin reaction melanin, and collagens fibers extracellular matrix simultaneously, they have...
Human Wharton's jelly stem cells (HWJSCs) are able to differentiate into skin and oral mucosa epithelial-like cells. In this work, we demonstrate for the first time capability of HWJSCs in vitro cornea a three-dimensional model.First, primary cell cultures HWJSCs, corneal epithelial cells, keratocytes were cultured orthotypic heterotypic human models generated with fibrin-agarose scaffolds. Then, differentiation was performed keratinocytic inductive medium system that allowed interaction...
We analyze the effect of nanoparticle concentration on physical properties magnetic hydrogels consisting polymer networks human fibrin biopolymer with embedded particles, swollen by a water-based solution. prepared these polymerization mixtures mainly plasma and nanoparticles OH- functionalization. Microscopic observations revealed that presented some cluster-like knots were connected several threads. By contrast, nonmagnetic homogeneous net-like structure only individual connections between...
The inclusion of magnetic nanoparticles into biopolymer matrixes enables the preparation field-responsive engineered tissues. Here we describe a synthetic route to prepare biocompatible core–shell nanostructures consisting polymeric core and shell, which are used for this purpose. We show that using architecture is doubly advantageous. First, gravitational settling nanocomposites slower because reduction composite average density connected light polymer core. Second, response can be tuned by...
Abstract Aims Arrhythmogenic right ventricular cardiomyopathy/dysplasia (ARVC/D) is a life-threatening entity with highly heterogeneous genetic background. Cardiac magnetic resonance (CMR) imaging can identify fibrofatty scar by late gadolinium enhancement (LGE). Our aim to investigate genotype–phenotype correlation in ARVC/D mutation carriers, focusing on CMR-LGE and myocardial fibrosis patterns. Methods results A cohort of 44 genotyped patients, 33 definite 11 borderline diagnosis, was...
Generation of biocompatible and biomimetic tissue-like biomaterials is crucial to ensure the success engineered substitutes in tissue repair. Natural able mimic structure composition native extracellular matrices typically show better results than synthetic biomaterials. The aim this study was perform an vivo time-course biocompatibility analysis fibrin-agarose hydrogels at histological, imagenological, hematological, biochemical levels. Tissue-like were produced by a controlled...
The generation of biomimetic and biocompatible artificial tissues is the basic research objective for tissue engineering (TE). In this sense, biofabrication scaffolds that resemble tissues' extracellular matrix an essential aim in field. Uncompressed nanostructured fibrin-agarose hydrogels (FAH NFAH, respectively) have emerged as promising TE, but their structure biomechanical properties must be improved order to broaden TE applications. Here, we generated characterized novel membrane-like...
Neural tissue engineering is focused on the design of novel biocompatible substitutes to repair peripheral nerve injuries. In this paper we describe a nanostructured fibrin-agarose bioartificial substitute (NFABNS), based hydrogels (FAHs) with human adipose-derived mesenchymal stem cells (HADMSCs). These NFABNSs were mechanically characterized and HADMSCs behaviour was evaluated using histological ultrastructural techniques. Mechanical characterization showed that resistant, flexible...