Generation of biocompatible and biomimetic tissue-like biomaterials is crucial to ensure the success engineered substitutes in tissue repair. Natural able mimic structure composition native extracellular matrices typically show better results than synthetic biomaterials. The aim this study was perform an vivo time-course biocompatibility analysis fibrin-agarose hydrogels at histological, imagenological, hematological, biochemical levels. Tissue-like were produced by a controlled...

Fibrin hydrogels made by self-assembly of fibrinogen obtained from human plasma have shown excellent biocompatible and biodegradable properties are widely used in regenerative medicine. The process can be triggered under physiological conditions the action thrombin, allowing injection pregel mixtures that been as cell carriers, wound-healing systems, bio-adhesives. However, access to is expensive fibrin gels limited mechanical properties, which make them unsuitable for certain applications....

Abstract The recent coronavirus disease (COVID‐19) forced pre‐university professionals to modify the educational system. This work aimed determine effects of pandemic situation on students' access medical studies by comparing performance students. We evaluated students enrolled in a subject taught first semester curriculum two pre‐pandemic academic years (PRE), post‐pandemic (POST), and an intermediate year (INT) using results final multiple‐choice exam. Consistency analysis among periods...

The fabrication of bioartificial tissue substitutes is a complex process that relies on the application innovative biomaterials and manufacturing techniques enabling generation cell-laden scaffolds mimicking natural interfaces. Among many biomaterials, gelatin methacryloyl (GelMA) hydrogels have shown great potential for 3D bioprinting-based engineering due to their high biocompatibility, biodegradability tuneable mechanical properties. In this study, use hybrid based GelMA highly purified...

Acellular nerve allografts (ANGs) represent a promising alternative in repair. Our aim is to improve the structural and biomechanical properties of biocompatible Sondell (SD) Roosens (RS) based ANGs using genipin (GP) as crosslinker agent ex vivo. The impact two concentrations GP (0.10% 0.25%) on Wistar rat sciatic nerve-derived was assessed at histological, biomechanical, biocompatibility levels. Histology confirmed differences between SD RS procedures, but not remarkable changes were...

Nerve autograft is the gold standard technique to repair critical nerve defects, but efficient alternatives are needed. The present study evaluated suitability of our novel Roosens-based (RSN) decellularized peripheral allografts (DPNAs) in 10-mm sciatic defect rats at functional and histological levels after 12 weeks. These DPNAs were compared with (AUTO) Sondell (SD) or Hudson (HD) based DPNAs. Clinical assessments demonstrated a partial regeneration all operated animals. RSN-based results...

In critical nerve gap repair, decellularized allografts are considered a promising tissue engineering strategy that can provide superior regeneration results compared to conduits. Decellularized nerves offer well-conserved extracellular matrix component has proven play an important role in supporting axonal guiding and peripheral regeneration. Up now, the known techniques time effort consuming. The present study, performed on rat sciatic nerves, aims at investigating novel decellularization...

As a reliable alternative to autografts, decellularized peripheral nerve allografts (DPNAs) should mimic the complex microstructure of native nerves and be immunogenically compatible. Nevertheless, there is current lack decellularization methods able remove cells without significantly altering extracellular matrix (ECM). The aims this study are firstly characterize ex vivo , in histological, biochemical, biomechanical ultrastructural way, three novel chemical-enzymatic protocols (P1, P2 P3)...

Traumatic nerve injuries are nowadays a significant clinical challenge and new substitutes with adequate biological mechanical properties in need. In this context, fibrin-agarose hydrogels (FA) have shown the possibility to generate tubular scaffolds promising results for repair. However, be clinically viable, these need possess enhanced properties. line, genipin (GP) crosslinking has demonstrated improve biomechanical good compared other crosslinkers. study, we evaluated impact of different...

Abstract Background Treatment of patients affected by severe burns is challenging, especially due to the high risk Pseudomonas infection. In present work, we have generated a novel model bioartificial human dermis substitute tissue engineering treat infected wounds using fibrin-agarose biomaterials functionalized with nanostructured lipid carriers (NLCs) loaded two anti- antibiotics: sodium colistimethate (SCM) and amikacin (AMK). Results show that tissue-like substitutes strong...

Patients with severe limbal damage and stem cell deficiency are a therapeutic challenge. We evaluated four decellularization protocols applied to the full-thickness half-thickness porcine limbus, we used two types recellularize decellularized limbi. The results demonstrated that all achieved efficient decellularization. However, method best preserved transparency composition of limbus extracellular matrix was use 0.1% SDS limbus. Recellularization epithelial line SIRC human adipose-derived...

Growth-associated protein 43 plays a key role in neurite outgrowth through cytoskeleton remodeling. We have previously demonstrated that structural damage of peripheral nerves induces growth-associated upregulation to promote growth cone formation. Conversely, the limited regenerative capacity central nervous system due an inhibitory environment prevents major changes and should be presumably associated with low levels expression. However, alterations nerve never been assessed using marker....

Hollow conduits of natural or synthetic origins have shown acceptable regeneration results in short nerve gap repair; however, are still not comparable with the current gold standard technique “autografts”. do provide a successful outcome when it comes to critical repair. Enriching lumen different extracellular materials and cells could better biomimicry regenerating environment is expected ameliorate conduit performance. In this study, we evaluated vivo using hollow chitosan enriched...

Hydrogels are polymeric biomaterials characterised by their promising biological and biomechanical properties, which make them potential alternatives for use in tendon repair. The aim of the present study was to generate vitro, determine therapeutic efficacy vivo, novel nanostructured fibrin-based hydrogels be used as an augmentation strategy surgical repair rat Achilles injuries. Fibrin, fibrin-agarose fibrin-collagen (NFH, NFAH NFCH, respectively) were generated properties cell-biomaterial...

Nervous system traumatic injuries are prevalent in our society, with a significant socioeconomic impact. Due to the highly complex structure of neural tissue, treatment these is still challenge. Recently, 3D printing has emerged as promising alternative for producing biomimetic scaffolds, which can lead restoration tissue function. The objective this work was compare different biomaterials generating 3D-printed scaffolds use engineering. For purpose, four thermoplastic biomaterials,...

Recent advances in tissue engineering offer innovative clinical alternatives dentistry and regenerative medicine. Tissue combines human cells with compatible biomaterials to induce regeneration. Shortening the fabrication time of used will contribute treatment improvement, biomaterial functionalization can be exploited enhance scaffold properties. In this work, we have tested an alternative biofabrication method by directly including oral mucosa explants within for generation bioengineered...

Wharton’s jelly stem cells (WJSC) from the human umbilical cord (UC) are one of most promising mesenchymal (MSC) in tissue engineering (TE) and advanced therapies. The cell niche is a key element for both, MSC fully differentiated tissues, to preserve their unique features. basement membrane (BM) an essential structure during embryonic development adult tissues. Epithelial BMs well-known, but similar structures present other histological structures, such as peripheral nerve fibers, myocytes...

Human artificial corneas (HAC) generated by tissue engineering recently demonstrated clinical usefulness in the management of complex corneal diseases. However, biological mechanisms associated to their regenerative potential need be elucidated. In present work, we HAC using nanostructured fibrin-agarose biomaterials with cultured epithelial and stromal cells, compared structure histochemical immunohistochemical profiles control native (CTR-C) limbus (CTR-L) determine level biomimicry these...

Because cartilage has limited regenerative capability, a fully efficient advanced therapy medicinal product is needed to treat severe damage. We evaluated novel biomaterial obtained by decellularizing sturgeon chondral endoskeleton tissue for use in engineering. In silico analysis suggested high homology between human and collagen proteins, ultra-performance liquid chromatography confirmed that both types of consisted mainly the same amino acids. Decellularized was recellularized with...

This study evaluates the cognitive structure and social behavior of tissue engineering (TE) based on a science mapping analysis. Understanding terms topics that play key role in development TE can help administrative authorities to better plan funding. Moreover, knowledge collaborative networks identification potential new opportunities for collaboration may enhance synergies scientific activities implement future approaches therapy.

Human skin keratinocyte primary cultures can be established from biopsies with culture media containing epithelial growth factor (EGF). Although current methods are efficient, optimization is required to accelerate the procedure and obtain these in less time. In present study, we evaluated effect of novel formulations based on EGF-loaded nanostructured lipid carriers (NLC). First, biosafety NLC recombinant human EGF (NLC-rhEGF) was verified immortalized keratinocytes cornea cells, two cancer...

Abstract Background Histology of human oral mucosa is closely related with its function and anatomical location, a proper characterization the masticatory could be very useful in periodontal pathology. Objective In present work, we have carried out comprehensive study order to determine main histological features parakeratinized (POM) orthokeratinized (OOM) using light electron microscopy. Methods To perform this, used several histological, histochemical immunohistochemical methods detect...

Number of publications has been widely used as a measure research output, especially academic and university research. in tissue engineering (TE) increased year by since early 1990s. However, after an exponential growth phase, recently increase at lower rates, suggesting consolidation process which reviews become relevant high-evidence document type. The aim this study is to perform scientometric evaluation published literature on TE assess the status scientific evolution confirm area....