A5073399750- Signaling Pathways in Disease
- Chronic Kidney Disease and Diabetes
- Nitric Oxide and Endothelin Effects
- Cell Adhesion Molecules Research
- Ion Transport and Channel Regulation
- Renal and related cancers
- Adipose Tissue and Metabolism
- Pulmonary Hypertension Research and Treatments
- Adipokines, Inflammation, and Metabolic Diseases
- Protein Kinase Regulation and GTPase Signaling
- Graphene and Nanomaterials Applications
- Cancer-related molecular mechanisms research
- Neuroinflammation and Neurodegeneration Mechanisms
- Calpain Protease Function and Regulation
- Dialysis and Renal Disease Management
- Renin-Angiotensin System Studies
- PI3K/AKT/mTOR signaling in cancer
- Obstructive Sleep Apnea Research
- Peptidase Inhibition and Analysis
- Caveolin-1 and cellular processes
- Heart Failure Treatment and Management
- Axon Guidance and Neuronal Signaling
- Phosphodiesterase function and regulation
- Advanced Glycation End Products research
- Phagocytosis and Immune Regulation
Fundación Renal
2018-2025
Instituto Ramón y Cajal de Investigación Sanitaria
2013-2025
Universidad de Alcalá
2016-2025
Instituto de Salud Carlos III
2014-2024
Comunidad de Madrid
2020-2023
Galicia Sur Biomedical Foundation
2023
Servicio Gallego de Salud
2023
Hospital Universitario Príncipe de Asturias
2019-2022
Biomedical Research Foundation
2020
Hospital Central de la Defensa Gómez Ulla
2018
The zeta isoform of protein kinase C (zeta PKC) has been shown to be involved in the maturation Xenopus oocytes and mitogenic signaling fibroblasts. PKC also regulates important transcription factor nuclear kappa B, most probably by phosphorylation inhibitory molecule I B. mechanisms that control activity are still poorly characterized. This is not activated diacylglycerol but potently stimulated vitro products phosphatidylinositol 3-kinase (PI 3-kinase), which suggests at least one critical...
Physiological responses to chronic hypoxia include polycythemia, pulmonary arterial remodeling, and vasoconstriction. Chronic causes hypertension leading right ventricular hypertrophy heart failure. During hypertension, arteries exhibit increased expression of smooth muscle-α-actin -myosin heavy chain. NFATc3 (nuclear factor activated T cells isoform c3), which is aCa2+-dependent transcription factor, has been recently linked muscle phenotypic maintenance through the regulation α-actin. The...
One of the main challenges aerial robots navigation in indoor or GPS-denied environments is position estimation using only available onboard sensors. This paper presents a Simultaneous Localization and Mapping (SLAM) system that remotely calculates pose environment map different low-cost commercial platforms, whose computing capacity usually limited. The proposed adapts to sensory configuration robot, by integrating state-of-the art SLAM methods based on vision, laser and/or inertial...
Pulmonary hypertension occurs with prolonged exposure to chronic hypoxia in both adults and neonates. The Ca 2+ -dependent transcription factor, nuclear factor of activated T cells isoform c3 (NFATc3), has been implicated hypoxia-induced pulmonary arterial remodeling adult mice. Therefore, we hypothesized that NFATc3 is required for neonatal aim this study was determine whether 1) mediates increases right ventricular systolic pressure mice; 2) mice exposed hypoxia; 3) involved hypertrophy...
Gastroenteropancreatic neuroendocrine tumors (GEP–NETs) raise difficult therapeutic problems despite the emergence of targeted therapies. Somatostatin analogs (SSA) remain pivotal drugs. However, tachyphylaxis and limited antitumoral effects observed with classical somatostatin 2 (sst2) agonists (octreotide lanreotide) led to development new SSA, such as pan sst receptor agonist pasireotide. Our aim was compare pasireotide octreotide on cell survival, chromogranin A (CgA) secretion, sst2...
This paper represents research in progress Simultaneous Localization and Mapping (SLAM) for Micro Aerial Vehicles (MAVs) the context of rescue and/or recognition navigation tasks indoor environments. In this kind applications, MAV must rely on its own onboard sensors to autonomously navigate unknown, hostile GPS denied environments, such as ruined or semi-demolished buildings. article aims investigate a SLAM technique that fuses visual information measurements from inertial measurement unit...
The fabrication of bioartificial tissue substitutes is a complex process that relies on the application innovative biomaterials and manufacturing techniques enabling generation cell-laden scaffolds mimicking natural interfaces. Among many biomaterials, gelatin methacryloyl (GelMA) hydrogels have shown great potential for 3D bioprinting-based engineering due to their high biocompatibility, biodegradability tuneable mechanical properties. In this study, use hybrid based GelMA highly purified...
Integrin-linked kinase (ILK) is a key scaffolding protein between extracellular matrix and the cytoskeleton has been implicated previously in pathogenesis of renal damage. However, its involvement mitochondrial dysfunction remains to be elucidated. We studied role ILK downstream regulations damage mitochondria function both vivo vitro, using folic acid (FA)-induced kidney disease model. Wild type (WT) conditional-knockdown (cKD-ILK) mice were injected with single intraperitoneal dose FA...
Diabetic nephropathy is characterized by the rapid onset of hypertrophy and ECM expansion. Previously, we showed that calcineurin phosphatase required for synthesis in cultured mesangial cells. Therefore, examined effect inhibition on renal accumulation streptozotocin-induced diabetic rats. After 2 wk diabetes, protein was increased whole cortex glomeruli conjunction with activity. Daily administration cyclosporin A blocked both Also associated upregulation nuclear localization substrate...
Abstract Cardiovascular disease is an important cause of death in patients with chronic kidney (CKD). Protein-bound uremic toxins, such as p -cresyl and indoxyl sulfate (IS), are poorly removed during hemodialysis, leading to vascular endothelial dysfunction leukocyte extravasation. These processes can be related dynamic adhesion structures called podosomes. Several studies have indicated the role integrin-linked kinase (ILK) accumulation integrin-associated proteins Here, we investigated...
Abstract Background Traditional biomarkers of chronic kidney disease (CKD) detect the in its late stages and hardly predict associated vascular damage. Integrin-linked kinase (ILK) is a scaffolding protein serine/threonine that plays multiple roles several pathophysiological processes during renal However, involvement ILK as biomarker CKD problems remains to be fully elucidated. Methods was induced by an adenine-rich diet for 6 weeks mice. We used inducible knockdown mice (cKD-ILK) model...
Chronic hypoxia (CH) activates the Ca 2+ -dependent transcription factor nuclear of activated T cells isoform c3 (NFATc3) in mouse pulmonary arteries. However, mechanism this response has not been explored. Since we have demonstrated that NFATc3 is required for CH-induced arterial remodeling, establishing how CH physiologically significant. The goal study was to test hypothesis endothelin-1 (ET-1) contributes activation. We propose requires increased smooth muscle cell (PASMC) intracellular...
Sleep apnea, defined as intermittent respiratory arrest during sleep, is associated with increased incidence of hypertension and peripheral vascular disease. Exposure rodents to brief periods hypercarbia/hypoxia (H-IH) sleep mimics the cyclical hypoxia-normoxia apnea. Endothelin-1, an upstream activator nuclear factor activated T cells (NFAT), H-IH. Therefore, we hypothesized that NFATc3 by H-IH required for H-IH-induced hypertension. Consistent this hypothesis, found (20 exposures per hour...
The development of insulin resistance is characterized by the impairment glucose uptake mediated transporter 4 (GLUT4). Extracellular matrix changes are induced when metabolic dysregulation sustained. present work was devoted to analyze possible link between extracellular-to-intracellular mediator integrin-linked kinase (ILK) and peripheral tissue modification that leads homeostasis impairment. Mice with general depletion ILK in adulthood (cKD-ILK) maintained a chow diet exhibited increased...
cGMP is generated in endothelial cells after stimulation of soluble guanylyl cyclase (sGC) by nitric oxide (NO) or particulate (pGC) natriuretic peptides (NP). We examined whether localized increases cytosolic have distinct regulatory roles on the contraction induced H 2 O treatment human umbilical vein cells. concentrations and temporal dynamics were different upon NO sGC C-type NP (CNP) activation pGC did not correlate with their relaxing effects measured as planar cell surface area...
Sleep apnea (SA) is defined as intermittent respiratory arrest during sleep and affects up to 20% of the adult population. SA also associated with an increased incidence hypertension peripheral vascular disease. Exposing rodents hypoxia mimics cyclical hypoxia/normoxia SA. We have previously shown that in mice rats induces ET-1 upregulation systemic hypertension. Furthermore, (IH) increases nuclear factor activated T cells isoform 3 (NFATc3) transcriptional activity aorta mesenteric...
The nitric oxide/soluble guanylyl cyclase (sGC) signal transduction pathway plays an important role in smooth muscle relaxation and phenotypic regulation. However, the transcriptional regulation of sGC gene expression is largely unknown. It has been shown that increases pulmonary arteries from chronic hypoxia-induced hypertensive animals. Since transcription factor NFATc3 required for upregulation hypertrophic/differentiation marker alpha-actin artery cells chronically hypoxic mice, we...
One of the clinical alterations observed in chronic renal disease (CRD) is impaired urine concentration, known as diabetes insipidus (DI). Tubulointerstitial fibrosis kidney also a pathological finding CRD and involves composition extracellular matrix (ECM). However, an association between these two events has not been elucidated. In this study, we showed that extracellular-to-intracellular scaffold protein integrin-linked kinase (ILK) regulates expression tubular water channel aquaporin-2...
The pathogenesis of diabetic retinopathy (DR) remains unclear but hyperglycemia is an established risk factor. Endothelial dysfunction and changes in Ca 2+ signaling have been shown to precede the onset DR. We recently demonstrated that high extracellular glucose activates /calcineurin-dependent transcription factor NFAT cerebral arteries aorta, promoting expression inflammatory markers. Here we show, using confocal immunofluorescence, expressed endothelium retinal microvessels readily...