A5058550136- Multiple Myeloma Research and Treatments
- Cellular Mechanics and Interactions
- Cell Adhesion Molecules Research
- Acute Myeloid Leukemia Research
- Chronic Lymphocytic Leukemia Research
- Protein Degradation and Inhibitors
- Chronic Myeloid Leukemia Treatments
- Histone Deacetylase Inhibitors Research
- Nuclear Structure and Function
- Bone health and treatments
- Blood disorders and treatments
- Mast cells and histamine
- Cancer Cells and Metastasis
- Glycosylation and Glycoproteins Research
- Cellular transport and secretion
- Erythrocyte Function and Pathophysiology
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Advanced Fluorescence Microscopy Techniques
- Peptidase Inhibition and Analysis
- Cardiomyopathy and Myosin Studies
- Sulfur Compounds in Biology
- Wnt/β-catenin signaling in development and cancer
- Microtubule and mitosis dynamics
- Calcium signaling and nucleotide metabolism
- Axon Guidance and Neuronal Signaling
University of Roehampton
2013-2025
King's College London
2006-2017
Otemae Hospital
2008-2011
University College London
2004-2006
Great Ormond Street Hospital
2006
University of Bristol
2006
St George's, University of London
2004
St George's Hospital
2004
Johnson University
2001
University of the Basque Country
1995-2000
Astrocytes, oligodendrocytes, and oligodendrocyte/type 2 astrocyte progenitors (O2A cells) can all produce molecules that inhibit axon regeneration. We have shown previously inhibition of growth by astrocytes involves proteoglycans. To identify inhibitory mechanisms, we created cell lines are permissive or nonpermissive showed cells chondroitin sulfate proteoglycans (CS-PGs). now tested these for the production function known large CS-PGs. The most line, Neu7, produces three CS-PGs in much...
Specific mutations in the human gene encoding Wiskott-Aldrich syndrome protein (WASp) that compromise normal auto-inhibition of WASp result unregulated activation actin-related 2/3 complex and increased actin polymerizing activity. These activating are associated with an X-linked form neutropenia intrinsic failure myelopoiesis increase incidence cytogenetic abnormalities. To study underlying mechanisms, active mutant WASpI294T was expressed by transfer. This caused enhanced delocalized...
Podosomes, highly dynamic adhesion structures implicated in cell motility and extracellular matrix degradation, are characteristic of certain cells the myeloid lineage a limited range other types. The nature mechanisms that regulate their high turnover unknown at present. cysteine protease calpain is involved regulation migration part by promoting either formation or disassembly sites. Despite fact many known substrates also structural components podosome complex, no studies have yet...
The Wiskott-Aldrich Syndrome protein (WASP) is an adaptor that essential for podosome formation in hematopoietic cells. Given 80% of identified patients result from mutations the binding site WASP-interacting-protein (WIP), we examined possible role WIP regulation architecture and cell motility dendritic cells (DCs). Our results show both actin cores containing WASP cortactin organization integrin integrin-associated proteins circular arrays, specific characteristics structure. We also found...
Staphylococcus aureus escapes from immune recognition by invading a wide range of human cells. Once the pathogen becomes intracellular, most important last resort antibiotics are not effective. Therefore, novel anti-infective therapies against intracellular S. urgently needed. Here, we have studied physiological changes induced in host cells during its proliferation. This is important, because exploits cell’s metabolism for own We find that severely depletes glucose and amino acid pools,...
Summary Progressive chronic lymphocytic leukaemia is characterized by the accumulation of neoplastic B‐cells in tissues and correlates with expression prognostic biomarkers, such as CD38, CD49d matrix metalloproteinase‐9 (MMP9), which are involved migration tissue invasion. In this study we investigated physical relationship between these molecules demonstrated that CD49d, MMP9 CD44 were physically associated a supramolecular cell surface complex. Our findings provide molecular basis for...
The Wiskott-Aldrich syndrome protein (WASp) is a key cytoskeletal regulator in hematopoietic cells. Covalent modification of conserved tyrosine by phosphorylation has emerged as an important potential determinant activity, although the physiological significance remains uncertain. In murine knockin model, mutation resulting inability to phosphorylate Y293 (Y293F) mimicked many features complete WASp-deficiency. Although phosphomimicking mutant Y293E conferred enhanced actin-polymerization,...
Abstract Host-directed therapeutics are a promising anti-infective strategy against intracellular bacterial pathogens. Repurposing host-targeted drugs approved by the FDA in US, MHRA UK and/or regulatory equivalents other countries, is particularly interesting because these commercially available, safe doses documented and they have been already for clinical purposes. In this study, we aimed to identify novel therapies Staphylococcus aureus , an opportunistic pathogen that able exploit host...
Summary Focal adhesions and podosomes are integrin‐mediated cell‐substratum contacts that can be visualized using interference reflection microscopy (IRM). Here, we have developed automated image‐processing procedures to quantify adhesion turnover from IRM images of live cells. Using time sequences images, produce maps reveal the spatial changes contain additional information on sequence these changes. Such were used characterize focal dynamics in mouse embryo fibroblasts lacking one or both...
Abstract Cardiovascular disease is an important cause of death in patients with chronic kidney (CKD). Protein-bound uremic toxins, such as p -cresyl and indoxyl sulfate (IS), are poorly removed during hemodialysis, leading to vascular endothelial dysfunction leukocyte extravasation. These processes can be related dynamic adhesion structures called podosomes. Several studies have indicated the role integrin-linked kinase (ILK) accumulation integrin-associated proteins Here, we investigated...
Wiskott–Aldrich syndrome (WAS) is an X-linked hematological disease characterized by immunodeficiency, eczema, and thrombocytopaenia, shows promise for treatment with hematopoietic stem cell gene therapy. The immunopathology of WAS attributable at least in part to defects migration localization as a result chemotactic, adhesive, chemokinetic defects. Whereas previous studies using either gammaretroviral or lentiviral vectors have demonstrated variable correction T-cell proliferation...