A5063038499- Periodontal Regeneration and Treatments
- Bone Tissue Engineering Materials
- Electrospun Nanofibers in Biomedical Applications
- Tissue Engineering and Regenerative Medicine
- Mesenchymal stem cell research
- Endodontics and Root Canal Treatments
- Dental Radiography and Imaging
- Oral microbiology and periodontitis research
- Genital Health and Disease
- Corneal Surgery and Treatments
- Corneal surgery and disorders
- Facial Rejuvenation and Surgery Techniques
- Connexins and lens biology
- Dental materials and restorations
- Hydrogels: synthesis, properties, applications
- Graphene and Nanomaterials Applications
- dental development and anomalies
- Nail Diseases and Treatments
- 3D Printing in Biomedical Research
- Oral and gingival health research
- Health and Medical Education
- Oral Health Pathology and Treatment
- Pluripotent Stem Cells Research
- Dupuytren's Contracture and Treatments
- Veterinary Pharmacology and Anesthesia
Universidad Antonio Nariño
2019-2022
Universidad de Granada
2012-2020
Instituto de Investigación Biosanitaria de Granada
2014-2015
Human Wharton's jelly stem cells (HWJSCs) are able to differentiate into skin and oral mucosa epithelial-like cells. In this work, we demonstrate for the first time capability of HWJSCs in vitro cornea a three-dimensional model.First, primary cell cultures HWJSCs, corneal epithelial cells, keratocytes were cultured orthotypic heterotypic human models generated with fibrin-agarose scaffolds. Then, differentiation was performed keratinocytic inductive medium system that allowed interaction...
to design calcium and zinc-loaded bioactive cytocompatible nanoparticles for the treatment of periodontal disease.PolymP-nActive were zinc or loaded. Biomimetic phosphate precipitation on polymeric particles was assessed after 7 days immersion in simulated body fluid, by scanning electron microscopy attached an energy dispersive analysis system. Amorphous mineral deposition probed X-ray diffraction. Cell viability performed using oral mucosa fibroblasts by: 1) quantifying liberated...
Dupuytren's disease is a fibro-proliferative characterized by disorder of the extracellular matrix (ECM) and high myofibroblast proliferation. However, studies failed to determine if whole palm fascia affected disease. The objective this study was analyze several components three types tissues—Dupuytren's diseased contracture cords (DDC), palmar clinically unaffected (NPF), normal forehand (NFF). Histological analysis, quantification cells recultured from each type tissue, mRNA microarrays...
Local anesthetic drugs are extensively used in dentistry. However, the cytotoxic effects of these pharmaceutical compounds remain unclear. In this work, we have evaluated cell viability and function human oral mucosa fibroblasts exposed to different concentrations lidocaine for increasing incubation times, using a global screening methods including structural, metabolic microanalytical analyses. Our results demonstrate that is able alter even at low although effect concentration was more...
Oral mucosa shortage may limit or condition some clinical approaches in maxillofacial, periodontal and implant treatment. The availability of a human oral model generated by tissue engineering could help clinicians to address the lack mucosa. In this work, we carried out sequential maturation differentiation study epithelial cell layer an artificial substitute based on fibrin-agarose biomaterials with fibroblasts keratinocytes.Histological, immunohistochemical gene expression analyses were...
Regenerative medicine is used for tissue healing, replace missing organs and tissues which are affected by aging, disease, or trauma both structurally functionally. Materials the key component of regenerative approaches because material can imitate native extracellular matrix those improve physical behavior properties new tissue. However, most available materials that purpose fail to provide adequate mechanical replicate original Thus, these applications still far from being enduring. This...
To identify genetic expression patterns that can be used to define an appropriate diagnostic algorithm of clinical use in periodontal disease. Total RNA was extracted from 13 samples corresponding normal human gingiva (NHG) and affected by disease (PDHG). A comprehensive gene analysis carried out microarray using Affymetrix Human Genome U133 plus 2.0 oligonucleotide arrays. Sixty-six probe sets (genes expressed sequence tags – EST) overexpressed all one the comparison groups, were for...
Abstract Introduction The success of endodontic treatment depends on a good knowledge the root canal system. Aim: purpose this study was to describe anatomical configuration mandibular first premolars using cone‐beam computed tomography (CBCT) in group Colombian population according Vertucci's classification, as well frequency presence variations. Materials and Methods A descriptive carried out 100 obtain CBCT images. length, curvature, canals, number location apical foramen, shape entrance...