A5101500787- Insect Resistance and Genetics
- Animal Virus Infections Studies
- Viral gastroenteritis research and epidemiology
- Virus-based gene therapy research
- Insect Pest Control Strategies
- CRISPR and Genetic Engineering
- Entomopathogenic Microorganisms in Pest Control
- Esophageal Cancer Research and Treatment
- Insect and Pesticide Research
- Plant Virus Research Studies
- RNA Interference and Gene Delivery
- Autophagy in Disease and Therapy
- Viral Infectious Diseases and Gene Expression in Insects
- Mosquito-borne diseases and control
- Genetically Modified Organisms Research
- Drug Transport and Resistance Mechanisms
- HIV Research and Treatment
- Immunotherapy and Immune Responses
- Viral Infections and Immunology Research
- Cancer Cells and Metastasis
- Esophageal and GI Pathology
- Animal Disease Management and Epidemiology
- Hepatitis B Virus Studies
- Immune Response and Inflammation
- Immune cells in cancer
Harbin Veterinary Research Institute
2018-2025
Chinese Academy of Agricultural Sciences
2018-2025
Central China Normal University
2015-2024
Peking University
2015-2024
Peking University Cancer Hospital
2015-2024
Nanjing Drum Tower Hospital
2020-2024
Zhejiang Cancer Hospital
2018-2023
Chinese Academy of Sciences
2023
University of Chinese Academy of Sciences
2021
Zhejiang University
2015
The intestinal microflora affects inflammation and immunity, not only locally at the mucosal level but also systemically, raising question of whether inflammatory processes that contribute to cancer its therapy. Prebiotics have been found play an antitumor role is limited gut. We investigated roles microbiota using Lewis lung mouse model. In mice treated with cisplatin combined ABX (an antibiotic cocktail vancomycin, ampicillin, neomycin), which can destroy host commensal microflora, tumor...
Porcine reproductive and respiratory syndrome (PRRS) is one of the most significant swine diseases. However, phylogenetic genomic recombination properties PRRS virus (PRRSV) have not been completely elucidated. In this study, we systematically compared differences in lineage distribution, recombination, NSP2 polymorphisms, evolutionary dynamics between North American (NA)-type PRRSVs China United States. Strikingly, found high frequency interlineage hot spots nonstructural protein 9 (NSP9)...
Multidrug resistance (MDR) poses a great challenge to cancer therapy. It is difficult inhibit the growth of MDR due its chemoresistance. Furthermore, cancers are more likely metastasize, causing high mortality among patients. In this study, nanomedicine RGD-NPVs@MNPs/DOX was developed by encapsulating melanin nanoparticles (MNPs) and doxorubicin (DOX) inside RGD peptide (c(RGDyC))-modified nanoscale platelet vesicles (RGD-NPVs) efficiently metastasis drug-resistant tumors via cells tumor...
PDCoV is a pathogen with the potential for transmission across diverse species, although mechanism of such host-switching events (from swine to other species) poorly understood. Here, we show that enters nonsusceptible cells but without efficient replication.
ABSTRACT We have previously shown that the nucleocapsid protein of SARS‐associated coronavirus (SARS‐CoV) activated cyclooxygenase‐2 (COX‐2) expression (1). In this study, we identified another viral protein, spike SARS‐CoV, which played an important role in virus‐stimulated COX‐2 after screening all genes from SARS‐CoV genome. found upstream calcium‐dependent PKC isozyme PKCα modulates downstream ERK/ NF‐κB pathway through influx extracellular Ca 2+ is induced by SARS‐CoV. The ERK/NF‐κB was...
ABSTRACT Highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) possesses greater replicative capacity pathogenicity than classical PRRSV. However, the factors that lead to enhanced replication remain unclear. In our study, an alignment of all available full-length sequences North American-type PRRSVs ( n = 204) revealed two consistent amino acid mutations differed between HP-PRRSV PRRSV were located at positions 519 544 in nonstructural protein 9. Next, a series...
Revealing the mechanisms of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) entry and cell-to-cell spread might provide insights for understanding underlying viral pathogenesis, tropism, virulence. The signaling pathways involved in SARS-CoV-2 spike-mediated fusion remain elusive. In current study, we found that macropinocytosis inhibitors significantly suppressed infection at both steps. We demonstrated required small GTPase Rac1 its effector kinase p21-activated 1 by...
Porcine reproductive and respiratory syndrome (PRRS) is one of the most economically important diseases affecting pig-raising industry. The PRRS virus (PRRSV) has high genetic diversity, partly owing to viral recombination. Some individual recombinant type 2 PRRSV (PRRSV-2) strains have been detected; however, sequence composition characteristics recombination hot spots potential driving forces for PRRSV-2 are still unreported. Therefore, all available genomic sequences (n = 949, including...
Transgenic plants that produce Bacillus thuringiensis (Bt) crystalline (Cry) toxins are cultivated worldwide to control insect pests. Resistance B. threatens this technology, and although different resistance mechanisms have been identified, some not completely elucidated. To gain new insights into these mechanisms, we performed multiple back-crossing from a 3000-fold Cry1Ac-resistant BtR strain cotton bollworm (Helicoverpa armigera), isolating 516-fold (96CAD). Cry1Ac in 96CAD was tightly...
Labeling viruses with high-photoluminescence quantum dots (QDs) for single virus tracking provides a visual tool to aid our understanding of viral infection mechanisms. However, efficiently labeling internal components without modifying the envelope and capsid remains challenge, existing strategies are not applicable most viruses. Here, we have devised strategy using clustered regularly interspaced short palindromic repeats (CRISPR) imaging system label nucleic acids Pseudorabies (PRV) QDs....
Bacillus thuringiensis Cry proteins are used worldwide for insect control. It was proposed that Cry-protoxins must be converted into activated toxin by proteases to bind midgut cell kill insects. However, also and insects have evolved resistance toxins suggesting an independent toxicity pathway. Cadherin (CAD) ABCC transporters recognized as important receptors proteins. Here we constructed different Helicoverpa armigera mutations in these CRISPR/Cas9. HaCAD-KO mutant showed much higher...
Abstract Background The global pork industry is continuously affected by infectious diseases that can result in large-scale mortality, trade restrictions, and major reductions production. Nevertheless, the cause of many pigs remains unclear, largely because commonly used diagnostic tools fail to capture full diversity potential pathogens pathogen co-infection common. Results We a meta-transcriptomic approach systematically characterize 136 clinical cases representing different disease...
Abstract Viral-induced infectious diseases represent a major health threat and their control remains an unachieved goal, due in part to the limited availability of effective anti-viral drugs measures. The use natural products drug manufacturing is ancient well-established practice. Marine organisms are known producers pharmacological agents. In this study, total 20 extracts from marine microorganisms were evaluated for antiviral activity. These tested against two mammalian viruses, herpes...
It is well known that insect larval midgut cadherin protein serves as a receptor of Bacillus thuringiensis (Bt) crystal Cry1Ac or Cry1Ab toxins, since structural mutations and downregulation cad gene expression are linked with resistance to toxin in several lepidopteran insects. However, the role Spodoptera frugiperda (SfCad) mode action Bt toxins remains elusive. Here, we investigated whether SfCad involved susceptibility Cry1Fa toxins. In vivo, knockout by CRISPR/Cas 9 did not increase...
Intracranial aneurysm (IA) is common and occasionally results in life-threatening hemorrhagic strokes. However, the cell architecture inflammation IA dome remain less understood.
Abstract Background The hepatitis B virus (HBV) is a major etiological factor of inflammation and damage to the liver resulting in hepatocellular carcinoma. Transcription factors play important roles disordered gene expression injury caused by HBV. However, molecular mechanisms behind this observation have not been defined. Results In study, we observed that circulating prostaglandin (PGE) 2 synthesis was increased patients with chronic infection, detected elevated cyclooxygenase (COX)-2...
Abstract Human immunodeficiency virus TAT plays an important role in the disregulation of cytokine production associated with neurological disorders that follow HIV infection. IL‐1β is one inflammatory cytokines secreted by immune‐activated monocytes/macrophages. Previous reports have shown extracellular stimulates expression However, little known about mechanisms and possible TAT‐responsive elements within promoter. The present study shows increases human monocytes; PLC‐PKC...