ATP-binding cassette transporter C2 (ABCC2) is known to be a receptor for Bacillus thuringiensis (Bt) toxins in several lepidopteran insects. Mutations the ABCC2 gene have been genetically linked field-evolved resistance Cry1F toxin from Bt Spodoptera frugiperda. Here we generated SfABCC2 knockout strain of S. frugiperda using CRISPR/Cas9 system provide further functional evidence role this susceptibility and Cry1F. Results bioassays showed that displayed 118-fold compared with parental DH19...

Abstract BACKGROUND The fall armyworm Spodoptera frugiperda is a major agricultural pest that has invaded the East Hemisphere since 2016, generating serious threat to food security worldwide including Africa and Asia. Cry toxins produced by Bacillus thuringiensis (Bt) have been shown be effective against this insect pest. In different ABC transporters (ABCC2 or ABCC3) involved as receptors of some Cry1 toxins. Here we analyzed role SfABCC2 SfABCC3 in toxicity Cry1Fa Cry1Ab RESULTS Two S....

ATP-binding cassette transporter B1 (ABCB1, or P-glycoprotein) is known to be an important participant in multidrug resistance mammals, and it also has been proved as a for some insecticides several lepidopteran insects, yet the precise function of this Spodoptera frugiperda unknown. Here, we generated SfABCB1 knockout strain S. using CRISPR/Cas9 system explore its potential roles determining susceptibility chemical Bt toxins. Bioassay results showed that beta-cypermethrin,...

This study focuses on the regulatory effects of genes encoding juvenile hormone (JH) receptor methoprene-tolerant (Met) and transcription factor krüppel homolog 1 (Kr-h1) reproductive capacity Coccinella septempunctata male adults. Met Kr-h1 expression levels were analyzed in males fed artificial diets with without JH by quantitative real-time PCR, reproduction RNA interference technology. 5- 10-day-old a JH-supplemented diet lower than those JH. 5-day-old adult was lacking but higher There...

Bacillus thuringiensis Cry proteins are used worldwide for insect control. It was proposed that Cry-protoxins must be converted into activated toxin by proteases to bind midgut cell kill insects. However, also and insects have evolved resistance toxins suggesting an independent toxicity pathway. Cadherin (CAD) ABCC transporters recognized as important receptors proteins. Here we constructed different Helicoverpa armigera mutations in these CRISPR/Cas9. HaCAD-KO mutant showed much higher...

Abstract When Coccinella septempunctata L. was fed on an artificial diet, development prolonged and survival reproduction were decreased, thus negatively affecting population growth. Juvenile hormone moulting hormones are important factors in insect development. This study aims to screen out the optimal that can solve problems such as slow growth of C. during mass rearing, improving their effectiveness a biological control agent. The effects juvenile analogues larval adult investigated by...

Abstract The potential role of the juvenile hormone receptor gene (methoprene-tolerant, Met) in reproduction Coccinella septempunctata L. (Coleoptera: Coccinellidae)(Coleoptera: Coccinellidae), was investigated by cloning, analyzing expression profiles quantitative real-time PCR, and via RNA interference (RNAi). CsMet encoded a 1518-bp open reading frames with predicted protein product 505 amino acids; latter contained 2 Per-Arnt-Sim repeat profile at acid residues 30–83 102–175. expressed...

Vip3Aa is a novel insecticidal protein secreted by Bacillus thuringiensis (Bt) during its vegetative growth stages. It has high activity against lepidopteran pests such as Spodoptera frugiperda, and no cross-resistance with Cry proteins. As new type of insecticide, it plays an important role in controlling agricultural pests. However, the mechanism toxin, especially definite receptors, have not been fully revealed. In this study, previously reported receptor genes Sf-FGFR Sf-SR-C were...

Schistosomiasis is a chronic parasitic disease that continues to be pressing public health problem in many developing countries. The primary pathological damage from the granuloma and fibrosis caused by egg aggregation, early treatment can effectively prevent occurrence of liver fibrosis. Therefore, it very important identify biomarkers used for diagnosis Schistosoma japonicum infection. In this study, label-free proteomics method was performed observe alteration proteins before infection, 1...

In this study, an orthogonal array design with 16 factors at two levels (216) was performed to develop artificial diet rearing the adults of seven spotted ladybird beetle Coccinella septempunctata. The parameters weight gain and survival rate adults, preoviposition period, fecundity hatching diet-fed were monitored. included: pork liver, infant formula, sucrose, olive oil, yolk, corn yeast powder, cholesterol, casein, casein hydrolysate, vitamin 65% juvenile hormone III, protein E, honey...

Abstract In order to improve the rearing of Coccinella septempunctata L. (Coleoptera: Coccinellidae), nutrients such as shrimp, pollen, honey, and lard were added basic artificial diet, effects diet on biological parameters digestive enzymes evaluated. The results show that beetles feeding supplemented exhibited pupation, emergence, fecundity, hatching rates 102.69%, 125.02%, 162.33%, 119.90% those supplied with respectively. addition shrimp pollen basal improved protease, trypsin,...

An artificial diet was developed for continuous maintenance of ladybird beetle (Coccinella septempunctata) adults. This is an important biocontrol agent greenhouse crops. Several biological parameters were measured and compared between colonies fed the over eight successive generations, aphids. Weight, survival rates from 1st to 4th instar, pupation rate, emergence rate similar both colonies, but sex ratio (♀:♂) diet-fed colony higher than that aphid-fed colony. These results suggest this...

Evolution of resistance to Cry proteins in multiple pest insects has been threatening the sustainable use Bacillus thuringiensis (Bt)-transgenic crops. Better understanding about mechanism is needed. Our preliminary study reported that transcription HaABCC3 was significantly decreased a near-isogenic line (LFC2) Cry1Ac-resistant strain (LF60) global Helicoverpa armigera. However, causality between downregulation and Cry1Ac remains be verified, regulatory underlying still unclear. In this...

The ladybird beetle, Coccinella septempunctata, is an important predator of soft-bodied insects. To further optimise artificial diet for rearing the we investigated effects juvenile hormone (JH), honey, shrimp, rape pollen, lard, and other factors on biological characteristics C. septempunctata. Survival pupation rates larvae were decreased when JH lard added to diet; however, addition increased egg hatching 73.70%, which was significantly higher than control (50.56%). Adding honeybee pupae...

As a newer cis-nitromethylene neonicotinoid pesticide at present, cycloxaprid has good industrialization prospects, including the management of imidacloprid-resistant populations, because this chemical have an excellent efficiency against rice planthoppers. Sogatella furcifera (Horváth) is most economically important pest worldwide and developed resistance to many insecticides. This study focused on expression change these genes, induced by cycloxaprid, involved in metabolic detoxification...

The insect predator Coccinella septempunctata can effectively control many types of pests, such as aphids, whiteflies, and small lepidopteran larvae. We previously found that C. fed an artificial diet showed diminished biological properties(e.g. fecundity, egg hatching rate, survival etc.) compared with those natural prey (Aphis craccivora), likely due to different nutritional characteristics the diet. In this study, we used transcriptome sequencing analysis identify nutrition-...

The white-backed planthopper, Sogatella furcifera (Horváth), causes substantial damage to crops by direct feeding or virus transmission, especially southern rice black-streaked dwarf virus, which poses a serious threat production. Cycloxaprid, novel cis-nitromethylene neonicotinoid insecticide, has high efficacy against planthoppers, including imidacloprid-resistant populations. However, information about the influence of cycloxaprid on S. (Hemiptera: Delphacidae) at molecular level is...

A new method to generate radar air intelligent information by using data mining techniques based on historical is proposed. This has two stages: One “filtering separation - piecewise fitting feature clustering". In this stage, the divided into actual true track and noise. Through computing second-order discrete curvature, decomposed several segments, such as straight line arc, which are fitted with multinomial subsequently. On basis, after analyzing characteristic vector of data, clustering...