A5100698359- interferon and immune responses
- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- NF-κB Signaling Pathways
- Circular RNAs in diseases
- RNA modifications and cancer
- RNA Interference and Gene Delivery
- Cancer Cells and Metastasis
- Cell death mechanisms and regulation
- Cell Image Analysis Techniques
- Epigenetics and DNA Methylation
- Head and Neck Cancer Studies
- Ubiquitin and proteasome pathways
- Cancer Research and Treatments
- Insect Resistance and Genetics
- Extracellular vesicles in disease
- Cancer-related gene regulation
- Advanced biosensing and bioanalysis techniques
- Nanoparticle-Based Drug Delivery
- Gut microbiota and health
- Peptidase Inhibition and Analysis
- Oral and Maxillofacial Pathology
- Bone Metabolism and Diseases
- Dendrimers and Hyperbranched Polymers
- Nanoplatforms for cancer theranostics
Shanghai Ninth People's Hospital
2013-2025
Shanghai Jiao Tong University
2013-2025
Wuhan Polytechnic University
2023-2024
First Affiliated Hospital of Anhui Medical University
2024
Anhui Medical University
2024
China Energy Engineering Corporation (China)
2023
National Clinical Research Center for Digestive Diseases
2022
Chengdu Research Base of Giant Panda Breeding
2020-2021
Shanghai Sixth People's Hospital
2021
National Clinical Research
2021
Cisplatin resistance is a major challenge for advanced head and neck cancer (HNC). Understanding the underlying mechanisms developing effective strategies against cisplatin are highly desired in clinic. However, how tumor stroma modulates HNC growth chemoresistance unclear. We show that cancer-associated fibroblasts (CAFs) intrinsically resistant to have an active role regulating cell survival proliferation by delivering functional miR-196a from CAFs cells via exosomes. Exosomal then binds...
Osteopontin (OPN), a chemokine-like protein, plays crucial role in the proliferation and metastasis of various cancers.However, how tumor stroma modulates expression neoplastic OPN multifaceted roles head neck cancer (HNC) are unclear.In this study, we tried to investigate bridging between cells.Methods: Immunohistochemical staining quantitative real-time PCR were used detect HNC tissues, correlations clinicopathologic features then analyzed.We co-culture assay study modulatory IL-6 on...
The lncRNA LINC00460 plays crucial roles in several epithelial cancers, although its mechanisms of action differ greatly different cellular contexts. In this study, we aimed to determine the potential clinical applications and elucidate by which affects development progression head neck squamous cell carcinoma (HNSCC).The biological functions were assessed cancer lines. subcellular localization was evaluated nuclear/cytoplasmic fractionation fluorescence situ hybridization. RNA pull-down...
Side population (SP) cells and their relationship to stem cell-like properties have been insufficiently studied in colorectal cancer (CRC). MicroRNAs (miRNAs) attracted much attention but roles the maintenance of SP phenotype remain unclear. The SPs from CRC cell lines primary cultures were analysed for properties. MiRNA microarray analysis identified miR-328 as a potential stemness miRNA phenotype. level expression clinical samples its correlation with fraction determined. Gain-of-function...
Head and neck cancer (HNC) is one of the most common deadly diseases worldwide. An increasing number studies have recently focused on malignant functions cancer-associated fibroblasts (CAFs) in numerous cancers. However, underlying mechanisms by which CAF-derived exosomes promote tumor progression need to be further elucidated. This study aims determine whether loss specific miRNAs may involved transformation HNC.MiRNA array real-time PCR assays were used analyze differential expression from...
In our previous studies, enhancer of zeste homolog 2 (EZH2) has been proven to be a key oncogenic driver in oral squamous cell carcinoma (OSCC). However, the regulatory mechanisms on EZH2 remain poorly understood OSCC. Here, through multi-transcriptomics, bioinformatics analysis, and quantitative reverse transcriptase polymerase chain reaction (qRT-PCR), co-expression network long noncoding RNA RC3H2 (RC3H2), microRNA-101-3p (miR-101-3p), were screened validated as competing endogenous...
Abstract Background Nuclear factor-kappa B (NF-κB) signaling constitutes a key event in the multistep process of carcinogenesis, progression and treatment many cancer types. However, significance NF-κB pathway for complex tissue-specific aspects head neck progression, such as invasion metastasis, is less understood. Methods The expression p65 squamous cell carcinoma (SCCHN) clinical specimens by immunohistochemistry. role activity was determined western blot, reporter assay EMSA analysis...
The epithelial-mesenchymal transition (EMT) is a crucial step in epithelial cancer invasion and metastasis. aims of this study were to investigate validate unidentified micro RNAs (miRNAs) that regulate EMT reveal their clinical relevance patients. By applying miRNA array screening natural phenotype cell line pair transforming growth factor β-induced model, we found miR-153 was markedly downregulated the cells underwent an EMT. A close association confirmed between inhibition phenotype, as...
Epithelial-to-mesenchymal transition (EMT) is a key step of the progression tumor cell metastasis. Recent work has demonstrated some miRNAs play critical roles in EMT. In this study, we focused on miR-300 regulating The expression levels were examined epithelial carcinoma cells that underwent an EMT using quantitative reverse transcription-PCR. role was investigated by transfection mimic or inhibitor natural epithelial-mesenchymal phenotype line pairs and transforming growth factor (TGF)...
Abstract Enhancer of zeste homolog 2 ( EZH 2) has been characterized as a critical oncogene and promising drug target in human malignant tumors. The current inhibitors strongly suppress the enhanced enzymatic function mutant some lymphomas. However, recent identification PRC 2‐ methyltransferase‐independent role indicates that complete suppression all oncogenic functions is needed. Here, we report unique 2‐targeting strategy by identifying gambogenic acid GNA ) derivative novel agent...
Oral squamous cell carcinoma (OSCC) is affected by the interaction between oral pathogen and holobionts, or combination of host its microbial communities. Studies have indicated structure feature microbiome in OSCC tissue saliva, relationships microbiota sites, stages remain unclear. In present study, (T), saliva (S) mouthwash (W) samples were collected from same subjects carried out study 16S sequencing. The results showed T group was significantly different S W groups with character lower...
Gambogic acid (GA) is a major active ingredient of gamboge, widely used traditional Chinese medicine that has been reported to be potent cytotoxic agent against some malignant tumors. Many studies have shown the NF-kappa B signaling pathway plays an important role in anti-apoptosis and drug resistance tumor cells during chemotherapy. In this study, effects mechanisms GA inhibitor celastrol on oral cancer were investigated.Three human squamous cell carcinoma lines, Tca8113, TSCC NT, treated...
Background Cyclin D1 (CCND1) has been associated with chemotherapy resistance and poor prognosis. In this study, we tested the hypothesis that CCND1 expression determines response clinical outcomes in locally advanced head neck squamous cell carcinoma (HNSCC) patients treated neoadjuvant followed by surgery radiotherapy. Methodology Findings 224 HNSCC were either cisplatin-based radiotherapy (neoadjuvant group, n = 100) or (non-neoadjuvant 124). was assessed immunohistochemistry. levels...
Abstract Histone lysine demethylase 6a (Kdm6a) mediates the removal of repressive trimethylation from histone H3 27 (H3K27me3) to activate target gene expression. Obesity is associated with metabolic inflammation, and adipose tissue macrophages (ATMs) are key players orchestrating inflammation. However, it still unclear whether Kdm6a pathway in ATMs regulates energy homeostasis. Here, we identified as a critical epigenetic switch that modulates macrophage polarisation further disrupts...
Aim: To design and fabricate a multifunctional drug-delivery nanoplatform for oral cancer therapy. Materials & methods: Polyethylene glycol-stabilized, PDPN antibody (PDPN Ab)- doxorubicin (DOX)-conjugated gold nanoparticles (AuNPs) were prepared evaluated their cytotoxicity antitumor efficacy in both chemotherapy photothermal Results: The obtained Ab)-AuNP-DOX system presents low toxicity, high drug loading capacity cellular uptake efficiency. Both vitro vivo experiments demonstrate that...
Abstract Gambogic acid (GA) is known for its anti‐cancer activity in a phase II clinical trial. However, the detailed molecular mechanisms of anti‐multidrug resistance remain unclear. The present study was designed to relationship between GA and multidrug‐resistant protein ATP‐binding cassette transporter B1 (ABCB1). dose dependently inhibited ABCB1 levels vitro Pgp‐Glo assay system increased cellular accumulation substrate adriamycin. Although had no significant influence on mRNA real‐time...
Abstract Background To identify new and useful candidate biomarkers in head neck squamous cell carcinoma (HNSCC), we performed a genome-wide survey found that Myelin lymphocyte-associated protein (MAL) was gene markedly down-regulated HNSCC. Hence, investigated the mechanism of MAL silencing effects on proliferation, invasion, apoptotic potential Results significantly 91.7% HNSCC specimens at mRNA level as compared with adjacent normal tissues ( P = 0.0004). Moreover, relative transcript...
Aims To investigate the expression of EMP1 in oral squamous cell carcinoma (OSCC) tissues and its correlation with available clinical parameters patients OSCC. Methods The mRNA levels were measured 18 paired OSCC corresponding adjacent normal using RT-PCR. Another 45 pairs samples selected to detect level quantitative RT-PCR (qRT-PCR). protein also evaluated 60 cases immunohistochemical staining. between was analysed non-parametric analysis. Results results qRT-PCR showed that, compared...
To elucidate the regulatory effect of circular RNA HIPK3 (circHIPK3) on progression gastric cancer (GC) by regulating Wnt/β-catenin pathway. We aim to reveal whether abnormal expression circHIPK3 could predict poor prognosis GC.CircHIPK3 level in GC tissues and paracancerous was determined quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). Its cell lines (BGC, MGC, SGC MKN) mucosal line (GES) examined as well. The correlation between overall survival analyzed Kaplan-Meier method....
Background Circulating tumor cells (CTCs) can survive in the circulation and return to primary tumors through a self-seeding process. However, mechanisms underlying CTCs escape from natural killer (NK) cell-mediated immune surveillance remain unclear. Method Self-seeded were isolated characterized using modified contralateral seeding model. A comparison of transcriptional profiles was performed between parental self-seeded cells. The molecular mechanism escaping NK cell demonstrated vitro...