A5011627793- Melanoma and MAPK Pathways
- CRISPR and Genetic Engineering
- RNA Interference and Gene Delivery
- Protein Kinase Regulation and GTPase Signaling
- Advanced biosensing and bioanalysis techniques
- PI3K/AKT/mTOR signaling in cancer
Mutant-specific inhibitors of KRASG12C, such as AMG510 (sotorasib) and MRTX849 (adagrasib), offer the unprecedented opportunity to inhibit KRAS, most frequently mutated heretofore undruggable oncoprotein. While clinical data are still limited, on-target mutations in KRASG12C at position 12 other sites emerging major drivers relapse. We identified additional that impact inhibitor sensitivity through a saturation mutagenesis screen NCI-H358 non–small-cell lung cancer (NSCLC) cell line. also...
Anti-sense oligonucleotides (ASOs) are modified synthetic single-stranded molecules with enhanced stability, activity, and bioavailability. They associate RNA through sequence complementarity can reduce or alter mRNA expression upon binding of splice site positions. To target in the nucleus cytoplasm, ASOs must cross membranes, a poorly understood process. We have performed an unbiased CRISPR/Cas9 knockout screen genetic reporter to identify genes that increase decrease resulting most...