Polycystic ovary syndrome (PCOS) is the most frequent endocrinopathy in women of reproductive age. It difficult to treat PCOS because its complex etiology and pathogenesis. Here, we characterized roles gut microbiota on pathogenesis treatments letrozole (a nonsteroidal aromatase inhibitor) induced rat model. Changes estrous cycles, hormonal levels, ovarian morphology by PCR-DGGE real-time PCR were determined. The results showed that rats displayed abnormal cycles with increasing androgen...

Fecal microbiota transplantation (FMT) is a promising therapy, despite some reports of adverse side effects. Bacterial consortia (BCT) for targeted restoration the intestinal ecosystem considered relatively safe and simple procedure. However, no systematic research has assessed effects FMT BCT on immune responses mucosal barrier in patients. We conducted complementary studies animal models BCT, provide recommendations improving clinical outcomes these treatments. To establish dysbiosis...

Accumulating evidence suggests that diet could shape the host gut microbiome composition.

Type 2 diabetes is a leading cause of morbidity and common risk several disorders. Identifying the microbial ecology changes essential for disease prediction, therapy, prevention. Thus, our study aimed at investigating intestinal microbiota among healthy type individuals exploring effect antidiabetic agents on gut bacterial flora. 24 (metformin, glimepiride, nontherapeutic subgroups; <inline-formula> <math xmlns="http://www.w3.org/1998/Math/MathML" id="M1"> <mi>N</mi> <mo>=</mo> <mn>8</mn>...

Background: The phylogenetic resolution within the Gloydius halys-intermedius Complex remains debatable due to following reasons: loci selection in previous studies varied between authors; limited dataset (1−5 mitochondrial or nuclear gene fragments); lack of sampling density; and nodal supports at specific nodes remain weak, specifically cognatus, G. halys, stejnegeri. Objectives: To revise taxonomic relationships Complex, we reconstructed molecular phylogeny performed species delimitation...

Abstract Antibiotic administration, while facilitating clearance of targeted infections, also perturbs commensal microbial communities. Previous studies have all focused on the effects short term use antibiotics. Here, we focus long antibiotic gut microbiota and immunity. BALB/c mice received saline or different doses ceftriaxone sodium (100, 200 400 mg/mL) via daily gavage for 150 days. Alterations fecal microbiota, small intestine histopathology, body weight, spleen index, serum IgG, mucus...

Objectives/Hypothesis Eosinophilic and noneosinophilic chronic rhinosinusitis with nasal polyps (ECRSwNP NECRSwNP) show distinguished clinical pathology, but their underlying mechanism remains unclear. We aimed to investigate the clinical, hematological, histopathological changes in (CRSwNP) endotypes its association microbiota. Study Design A comparative cross‐sectional study. Methods study of 46 patients CRSwNP (34.69 ± 16.39 years old) who underwent endoscopic sinus surgery were recruited...

Abstract Background Globally, urogenital and intestinal parasitosis remain significant health challenges. They are associated with rising morbidity, death, many harmful outcomes. A little is known concerning type 2 diabetes mellitus. Our study planned to investigate the parasitic infections among patients compare non-diabetic (Control) individuals examine intensity of helminthiasis in both groups. Methods At Kosti Teaching Hospital (Sudan), 300 Urine stool samples have collected from 150...

Several studies have confirmed that the pathophysiological progression of Alzheimer's disease (AD) is closely related to changes in intestinal microbiota; thus, modifying microbiota has emerged as a new way treat AD. Effective interventions for gut include application probiotics and other measures such fecal transplantation (FMT). However, ignores intestine complete microecosystem with competition among microorganisms. FMT also issues when applied patient treatment. In previous study, we...

Currently, several studies propose that the dominant intestinal bacteria are core flora. Besides keeping homeostasis of environment, microflora also plays a role in body metabolism, production some vitamins, and control barrier function. The study aimed to investigate jejunum microbiota diabetic rats as well it's relationship with Ceftriaxone sodium-mediated gut dysbiosis, parameters, permeability. Thirty-two Wistar (Male) were enrolled divided into four groups (A, B, C, D; N = 8)....

Duck-origin goose parvovirus (D-GPV) is the causative agent of beak atrophy and dwarfism syndrome (BADS), characterized by growth retardation, skeletal dysplasia persistent diarrhea. However, pathogenic mechanism D-GPV remains undefined. Here, we first reported gut microbiome diversity infected Cherry Valley ducks. In investigation for influence infection on microbiota through a period infection, found that caused dysbiosis reducing prevalence dominant genera decreasing microbial diversity....

Duck-origin parvovirus disease is an epidemic mainly caused by duck-origin goose (D-GPV), which characterized beak atrophy and dwarfism syndrome. Its main symptoms are persistent diarrhea, skeletal dysplasia, growth retardation. However, the pathogenesis of Cherry Valley ducks infected D-GPV has not been studied thoroughly. To perceive distribution in intestinal tract, morphological development, permeability, inflammatory cytokines ducks, expression tight junction protein, infection was...

Staphylococcus aureus (S. aureus), an important opportunistic pathogen in human and animal, causes a series of diseases the impairing immunity host even then death. Alpha-hemolysin (Hla), primary virulence factor, plays major role pathogenic progress S. aureus, especially pneumonia. Prim-O-glucosylcimifugin (POG), nature chromone compound, is active ingredient many Chinese Medicines. In this study, POG investigated inhibitory effect secretion Hla strain USA300 at subinhibitory...

Aim: To investigate the effects of a defined bacterial consortium on trinitrobenzenesulfonic acid (TNBS)-induced colitis and intestinal dysbiosis in rats. Materials & methods: Rats with TNBS-induced were treated ceftriaxone and/or mixture ten strains isolated from mouse feces for continuous 24 days. Macroscopic histopathological parameters colonic tissue compared, as myeloperoxidase enzyme activity cytokine levels. Patterns microbiota assessed by PCR-denaturing gradient gel electrophoresis,...

Summary A fragment of mitochondrial DNA (mtDNA) control region (∼700 bp) was sequenced in 104 individuals from 20 breeds (three Chinese domestic breeds, five recently derived and 12 introduced breeds) rabbits, Oryctolagus cuniculus . Nineteen sites were polymorphic, with 18 transitions one insertion/deletion, eight haplotypes (A1, A2, A3, A4, A5, A6, A7 A8) identified. Haplotype A1 the most common occurred 89 individuals. In 25 only haplotype observed, while four A5 A6) found 26 A2 shared by...

Abstract The large numbers of human intestinal microorganisms have a highly co-evolved relationship with the immune system. Dysbacteriosis microbiota induces alterations responses, and is closely related to disease development. Peyer’s patches are sensors in intestine which exert essential functions during development inflammatory disease. However, interactions between commensal bacteria PPs been poorly characterized. In this study, changes lymphocyte subpopulations production cytokines mice...

A water‑soluble polysaccharide fraction (PPSB) was fractionated from Physalis alkekengi var. francheti and purified using DEAE‑52 Cellulose Sephadex G‑200 chromatography. The physicochemical properties of PPSB its molecular activities involved in the improvement intestinal microflora balance were evaluated. (12.5‑25.0 mg/ml) shown to promote growth Lactobacillus delbrueckii inhibit Escherichia coli. Denaturing gradient gel electrophoresis analysis identified Bacteroides, Lactobacillus,...

ABSTRACT Lung cancer is the leading cause of death. Better understanding factors and pathways involved in lung needed to improve diagnose treatment strategies. Recent studies have provided insights into possible correlation between intestinal dysbiosis development. Although immunological relationship gut had been suggested by many researches, however, date, no study investigated characterization microbiome naïve patients, whether it distinct from that health individuals contribute onset...

Streptococcus mutans (S. mutans) is the primary etiological agent of human dental caries. A gene (SMU_1525) encoding MurA was identified in S. UA159 (ATCC700610). The deduced amino-acid sequence has homology (51% identity) with E. coli protein (AAC76221). In this study, we cloned murA gene, expressed soluble recombinant BL21(DE3). purified by affinity chromatography. molecular weight about 45.6 kD. activity high-pressure liquid chromatography (HPLC) and shown to have UDP-N-acetylglucosamine...

산화스트레스 유도성 SWPA2 프로모터 조절하에 항산화 효소 SOD와 APX 유전자를 동시에 엽록체에 발현시킨 형질전환 감자 (품종 수미)를 대상으로 methyl viologen (MV) 처리에 의해 유도되는 내성을 잎절편체, 소식물체 및 식물체 수준에서 조사하였다. 잎 절편에 <TEX>$3{\mu}M$</TEX> MV를 처리하였을 때 SSA 식물체의 잎절편체는 비형질전환 (NT) 식물체에 비해 40% 정도 상해를 적게 받았다. MV에 의한 조사하기 위하여 SSA감자 shoot을 <TEX>$0.3{\mu}M$</TEX> MV 첨가 배지에 배양하였을 뿌리의 생장에서 내성이 나타났다. 또한 온실에서 4주 생장한 <TEX>$350{\mu}M$</TEX> 경우에도 식물체는 NT 약 75% 손상을 입은 것으로 나타내었다. 추후 식물체를 이용하여 건조, 고온 등의 복합재해에 분석이 진행되어야 할 것이며 그 결과 복합스트레스 내성 품종 (수미)을 개발할 수 있을 기대한다....