A5079479145- Advanced Fluorescence Microscopy Techniques
- Receptor Mechanisms and Signaling
- Photoreceptor and optogenetics research
- bioluminescence and chemiluminescence research
- Gene Regulatory Network Analysis
- Protein Kinase Regulation and GTPase Signaling
- Pancreatic function and diabetes
- Cell Image Analysis Techniques
- Signaling Pathways in Disease
- Advanced biosensing and bioanalysis techniques
- CRISPR and Genetic Engineering
- Advanced Biosensing Techniques and Applications
- Protein Degradation and Inhibitors
- RNA Research and Splicing
- Phosphodiesterase function and regulation
- Ubiquitin and proteasome pathways
- Metabolism, Diabetes, and Cancer
- Retinal Development and Disorders
- Neuroscience and Neuropharmacology Research
- Photosynthetic Processes and Mechanisms
- Viral Infectious Diseases and Gene Expression in Insects
- RNA modifications and cancer
- Click Chemistry and Applications
- Monoclonal and Polyclonal Antibodies Research
- Photochromic and Fluorescence Chemistry
University of California, San Diego
2015-2024
Kindred Hospital Rancho
2023
Johns Hopkins Medicine
2011-2015
Johns Hopkins University
2009-2015
George Washington University
2004
AMP-activated protein kinase (AMPK) is a master regulator of cellular energetics which coordinates metabolism by phosphorylating plethora substrates throughout the cell. But how AMPK activity regulated at different subcellular locations for precise spatiotemporal control over unclear. Here we present sensitive, single-fluorophore reporter (ExRai AMPKAR), reveals distinct kinetic profiles mitochondria, lysosome, and cytoplasm. Genetic deletion canonical upstream liver B1 (LKB1) results in...
Signaling networks are spatiotemporally organized to sense diverse inputs, process information, and carry out specific cellular tasks. In β cells, Ca 2+ , cyclic adenosine monophosphate (cAMP), Protein Kinase A (PKA) exist in an oscillatory circuit characterized by a high degree of feedback. Here, we describe mode regulation within this involving spatial dependence the relative phase between cAMP, PKA, . We show that mouse MIN6 nanodomain clustering -sensitive adenylyl cyclases (ACs) drives...
The involvement of the putative glutamate receptor 1.1 (AtGLR1.1) gene in regulation abscisic acid (ABA) biosynthesis and signaling was investigated Arabidopsis. Seeds from AtGLR1.1-deficient (antiAtGLR1.1) lines had increased sensitivity to exogenous ABA with regard effect hormone on inhibition seed germination root growth. Seed germination, which inhibited by an animal ionotropic antagonist, 6,7-dinitroquinoxaline-2,3-[1H,4H]-dione, restored co-incubation inhibitor biosynthesis, fluridone....
Regulators of calcineurin (RCANs) in fungi and mammals have been shown to stimulate inhibit signaling vivo through direct interactions with the catalytic subunit phosphatase. The dual effects RCANs on were examined by performing structure-function analyses yeast Rcn1 human RCAN1 (a.k.a. DSCR1, MCIP1, calcipressin 1) proteins expressed at a variety different levels yeast. At high expression, inhibitory required degenerate PxIxIT-like motif novel LxxP motif, which may be related...
Calcineurin is responsible for mediating a wide variety of cellular processes in response to dynamic calcium (Ca2+) signals, yet the precise mechanisms involved spatiotemporal control calcineurin signaling are poorly understood. Here, we use genetically encoded fluorescent biosensors directly probe role cytosolic Ca2+ oscillations modulating activity dynamics insulin-secreting MIN6 β-cells. We show that induce distinct temporal patterns cytosol and plasma membrane vs at ER mitochondria these...
Ubiquitination is essential for protein degradation and signaling pivotal to many physiological processes. of a subset G-protein-coupled receptors (GPCRs) by the E3 ligase NEDD4-2 required p38 activation, but how GPCRs activate promote ubiquitin-mediated not known. Here, we report that GPCR protease-activated receptor-1 (PAR1) stimulates c-Src-mediated tyrosine phosphorylation activation endothelial barrier disruption. Using mass spectrometry, identified unique phosphorylated (Y)-485 within...