A5100419740- Neuroscience and Neuropharmacology Research
- Advanced Fluorescence Microscopy Techniques
- Cell Image Analysis Techniques
- Neuroinflammation and Neurodegeneration Mechanisms
- Spectroscopy Techniques in Biomedical and Chemical Research
- Spinal Cord Injury Research
- PI3K/AKT/mTOR signaling in cancer
- Neurological Disease Mechanisms and Treatments
- Pain Mechanisms and Treatments
- Pharmacological Receptor Mechanisms and Effects
- Intermetallics and Advanced Alloy Properties
- Plant-based Medicinal Research
- Photoacoustic and Ultrasonic Imaging
- Genetics and Neurodevelopmental Disorders
- Animal Genetics and Reproduction
- Medical Image Segmentation Techniques
- Tuberous Sclerosis Complex Research
- Transcranial Magnetic Stimulation Studies
- Image Processing Techniques and Applications
- Traumatic Brain Injury and Neurovascular Disturbances
- Hearing, Cochlea, Tinnitus, Genetics
- Anesthesia and Neurotoxicity Research
- Mathematical Dynamics and Fractals
- Receptor Mechanisms and Signaling
- Optical Imaging and Spectroscopy Techniques
Chinese Academy of Medical Sciences & Peking Union Medical College
2024
Hebei University of Technology
2024
Air Force Medical University
2022-2024
Beijing Technology and Business University
2024
Shenzhen University
2023-2024
National Health and Family Planning Commission
2019-2023
Peking University
2003-2023
Chinese Institute for Brain Research
2018-2023
Johns Hopkins Medicine
2005-2023
Johns Hopkins University
2005-2023
Significance Basal lateral amygdala and ventral CA1 are critical sites during chronic stress-induced depression. Here we demonstrated that AMPARs mediated weakening of pBLA-vCA1 innervation in mice subjected to CUMS. Stimulation via chemogenetics or administration cannabidiol (CBD) could reverse synaptosomal AMPAR decrease alleviate CUMS-induced depressive-like behaviors. These findings highlighted the importance an animal model depression revealed potential for CBD major treatment.
The histone methyltransferase enhancer of zeste 2 polycomb repressive complex subunit (EZH2)-mediated trimethylation H3 lysine 27 (H3K27me3) regulates neural stem cell proliferation and fate specificity through silencing different gene sets in the central nervous system. Here, we explored function EZH2 early post-mitotic neurons by generating a neuron-specific Ezh2 conditional knockout mouse line. results showed that lack neuronal led to delayed migration, more dendritic arborization,...
Abstract Target of rapamycin (TOR) is a conserved regulator cell growth and metabolism that integrates energy, factor, nutrient signals. The 280-kDa TOR protein functions as the catalytic component two large multiprotein complexes consists an N-terminal HEAT-repeat domain C-terminal Ser/Thr kinase domain. Here we describe allelic series mutations in Drosophila Tor gene show combinations HEAT domains display rare genetic phenomenon intragenic complementation, which or more defective proteins...
Genetic screens in invertebrates have discovered many synaptogenic genes and pathways. However, similar genetic studies not been possible mammals. We optimized an automated high-throughput platform that employs liquid handling imaging of primary mammalian neurons. Using this platform, we screened 3,200 shRNAs targeting 800 proteins. One the hits identified was LRP6, a coreceptor for canonical Wnt ligands. LRP6 regulates excitatory synaptogenesis is selectively localized to synapses. In vivo...
Abstract Background Blood–spinal cord barrier (BSCB) disruption is a key event after spinal injury (SCI), which permits unfavorable blood-derived substances to enter the neural tissue and exacerbates secondary injury. However, limited mechanical impact usually followed by large-scale BSCB in SCI. How propagated along acute period of SCI remains unclear. Thus, strategies for appropriate clinical treatment are lacking. Methods A contusion mouse model was established wild-type LysM-YFP...
Second-harmonic generation (SHG) is a noninvasive imaging technique that enables the exploration of physiological structures without use an exogenous label. However, traditional SHG limited by optical diffraction, which restricts spatial resolution. To break this limitation, we developed novel approach called multifocal structured illumination microscopy-SHG (MSIM-SHG). By combination with MSIM, SHG-based super-resolution material molecules can be achieved, and has wide range applications...
Glucagon-like peptide-1 receptor (GLP-1R) holds pivotal importance as a therapeutic target for type 2 diabetes (T2D) and obesity. Several oral small-molecule agonists targeting GLP-1R have been developed to date. Nevertheless, these suffer from several limitations, including low potency, poor pharmacokinetics, unfavorable safety profiles. Here, we report the discovery of compound 29 (DA-302168S), which exhibits higher potency both in vitro/in vivo while mitigating risk drug-drug interaction...
The degree to which intrathecally administered morphine can alleviate hypersensitivity in animals after peripheral nerve injury is controversial, and the mechanisms by works these circumstances are uncertain. In normal animals, induces adenosine release, vitro data suggest that this link disrupted injury. Therefore, using a controlled, blinded study design, authors tested intrathecal efficacy rats with role of spinal A1 receptors action morphine.Male underwent catheter implantation lumbar...
Introduction: Maternal immune activation (MIA) is closely related to the onset of autism-like behaviors in offspring, but mechanism remains unclear. can influence offspring’s development and behaviors, as indicated both human animal studies. We hypothesized that abnormal maternal MIA dams might be other factors leading delayed offspring. Methods: To verify our hypothesis, we analyzed poly(I:C)-induced dam’s postpartum behavior serum levels several hormones behavior. Pup’s developmental...
Abstract Traditionally, spatially-resolved photoluminescence (PL) has been performed using a point-by-point scan mode with both excitation and detection occurring at the same spatial location. But availability of high quality detector arrays like CCDs, an imaging become popular for performing PL. By illuminating entire area interest collecting data simultaneously from all locations, measurement efficiency can be greatly improved. However, this new approach proceeded under implicit assumption...
Dexmedetomidine (Dex), a highly selective α2-adrenergic receptor agonist, has been shown to attenuate systemic inflammatory response induced by lipopolysaccharide (LPS). The protective effects of Dex may reportedly be due the activation α7 nicotinic acetylcholine (α7nAChR)-dependent cholinergic anti-inflammatory pathway. Spleen play pivotal role in neural However, little is known about specific function spleen against sepsis. To investigate treatment sepsis, we studied preemptive...