A5100444443- Spectroscopy Techniques in Biomedical and Chemical Research
- Advanced Fluorescence Microscopy Techniques
- Advanced Fiber Laser Technologies
- Polyoxometalates: Synthesis and Applications
- Spectroscopy and Chemometric Analyses
- Photoacoustic and Ultrasonic Imaging
- Metal-Organic Frameworks: Synthesis and Applications
- Optical Coherence Tomography Applications
- Optical Imaging and Spectroscopy Techniques
- Advanced Fiber Optic Sensors
- Radio Frequency Integrated Circuit Design
- Photonic Crystal and Fiber Optics
- Advanced Electron Microscopy Techniques and Applications
- Advanced Photonic Communication Systems
- Optical Network Technologies
- Microwave Engineering and Waveguides
- melanin and skin pigmentation
- Force Microscopy Techniques and Applications
- Optical Polarization and Ellipsometry
- Lipid Membrane Structure and Behavior
- Atomic and Subatomic Physics Research
- Cold Atom Physics and Bose-Einstein Condensates
- Photonic and Optical Devices
- Advanced Nanomaterials in Catalysis
- Zebrafish Biomedical Research Applications
National Formosa University
2025
Chongqing University of Technology
2024
China Academy of Engineering Physics
2021-2024
Shanghai East Hospital
2024
Ministry of Agriculture and Rural Affairs
2024
China Agricultural University
2024
University of Electronic Science and Technology of China
2010-2024
Henan Tianguan Group (China)
2024
Huzhou University
2023-2024
Ministry of Transport
2024
Inorganic nanoparticles are ideal precursors for engineering barcodes rapidly detecting diseases. Despite advances in the chemical design of these barcodes, they have not advanced to clinical use because lack sensitivity and cost-effective due requirement a large read-out system. Here we combined recent quantum dot barcode technology with smartphones isothermal amplification engineer simple low-cost chip-based wireless multiplex diagnostic device. We characterized analytical performance this...
Edge computing provides off-load and application services close to end-users, greatly reducing cloud pressure communication overhead. However, wireless edge networks still face the risk of network attacks. To ensure security networks, we present Federated Learning-based Attention Gated Recurrent Unit (FedAGRU), an intrusion detection algorithm for networks. FedAGRU differs from current centralized learning methods by updating universal models rather than directly sharing raw data among...
Using single-molecule displacement/diffusivity mapping (SM
In this study, the phenomenon of stability-activity trade-off, which is increasingly recognized in enzyme engineering, was explored. Typically, enhanced stability enzymes correlates with diminished activity. Utilizing Rosa roxburghii copper–zinc superoxide dismutase (RrCuZnSOD) as a model, single-site mutations were introduced based on semirational design derived from consensus sequences. The initial set mutants selected activity, followed by combinatorial mutation. This approach yielded two...
ABSTRACT We are developing optical methods based on near infra‐red Raman spectroscopy and fluorescence photon migration for diagnosis localization of breast cancer. demonstrate the ability to classify accurately normal, benign malignant tissues, an important step in spectroscopic needle probes as a tool improving accuracy biopsy. also show that imaging can be used localize small fluorescent objects imbedded thick turbid medium with realistic properties, thus demonstrating potential this...
The processing of tyrosinase, which catalyzes the limiting reaction in melanin synthesis, was investigated melan-p1 melanocytes, are null at p locus. Endoglycosidase H digestion showed that a significant fraction tyrosinase retained endoplasmic reticulum. This retention could be rescued either by transfection cells with an epitope-tagged wild-typep transcript or treatment bafilomycin A1 ammonium chloride. We found reticulum contains amount protein, thus supporting role for within this...
Abstract We experimentally demonstrated the use of random plasmonic nano-islands for optical trapping and assembling particles live cells into highly organized pattern with low power density. The observed effect is attributed to net contribution due near-field force long-range thermophoretic force, which overcomes axial convective drag while lateral convection pushes target objects zone. Our work provides a simple platform on-chip manipulation nano- micro-sized may find applications in...
Residues of the psychoactive drug diazepam (DZP) may pose potential risks to fish in aquatic environments, especially by disrupting their behavioral traits. In this study, female and male zebrafish were subjected chronic exposure (21 days) sublethal doses (120 12 µg/L) DZP, aimed compare characteristics responses DZP exposure, investigate possible links between those variations brain γ-aminobutyric acid (GABA) acetylcholinesterase (AChE) levels. Chronic significantly decreased swimming...
Abstract The multiplexing capability of fluorescence microscopy is severely limited by the broad spectral width. Spectral imaging offers potential solutions, yet typical approaches to disperse local emission spectra notably impede attainable throughput. Here we show that using a single, fixed detection band, through frame-synchronized fast scanning excitation wavelength from white lamp via an acousto-optic tunable filter, up six subcellular targets, labeled common fluorophores substantial...
Abstract High efficacy and low toxicity are critical for cancer treatment. Polyoxometalates (POMs) have been reported as potential candidates therapy. On accounts of the slow clearance POMs, leading to long-term toxicity, clinical application POMs in treatment is restricted. To address this problem, a degradable organoimido derivative hexamolybdate developed by modifying it with cleavable organic group, its degradation. Of note, exhibits favourable pharmacodynamics towards human malignant...
Diffusion properties notably determine the behavior of biomembranes. Here we report concurrent nanoscale fine-mapping membrane topography, diffusivity, and packing order in live mammalian cells through a synergy single-molecule super-resolution methods. By identifying bright, lipophilic fluorescence turn-on probe that enables sustained imaging cellular membranes under stroboscopic excitation, accumulate positions transient displacements >106 molecules to achieve topography diffusivity...
Recent studies have sparked debate over whether catalytic reactions enhance the diffusion coefficients
Spatiotemporal heterogeneities in the polarity of intracellular LDs play essential roles cellular function but remain largely unexplored. Here, we introduce excitation spectral phasor microscopy (ExSPM), which combines high-throughput imaging with analysis to detect subtle shifts fluorophores response local environments exceptional sensitivity and spatiotemporal resolution. Using lipophilic dye Nile Red, reveal substantial dynamic changes LD within single mammalian cells. We further...
Cellular membranes orchestrate critical processes such as molecular transport and signal transduction, both regulated by the lateral mobility of lipids proteins. However, resolving nanoscale diffusional heterogeneities elucidating their underlying mechanisms remains a formidable challenge due to membrane's intricate architecture compositional diversity. Here, we present point-cloud single-molecule diffusivity mapping (pc-SMdM), cutting-edge super-resolution technique that offers data format...
A single-cell diagnostic technique for epithelial cancers is developed by utilizing laser trapping and Raman spectroscopy to differentiate cancerous normal cells. Single-cell suspensions were prepared from surgically removed human colorectal tissues following standard primary culture protocols examined in a near-infrared laser-trapping system, where living cells investigated one one. model was built on the spectral data obtained 8 patients validated 2 new patients. Our has potential...